A1JNX6 (PROA_YERE8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	YE3201

Organism

Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) [Complete proteome] [HAMAP]

Taxonomic identifier

393305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000050004

Sequences

	Sequence		Length	Mass (Da)
	A1JNX6 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 8EA76788A67ECFE3	FASTA	419	45,176
	10 20 30 40 50 60 MSMLEQMGKA AKQASWQLAM LSTAKKNQAL AVIANLLESE SQAILQANEQ DMAAARDSGM 70 80 90 100 110 120 SEALLDRLLL SPARLAAIAN DVRQVCRLND PVGRVIDGSL LDSGLKLERR RVPLGVIGVI 130 140 150 160 170 180 YEARPNVTID VASLCLKTGN AVILRGGKET HHTNQATVKV IQQALEQCGL PPAAVQAIES 190 200 210 220 230 240 PDRELVTQLL RMDNYIDMLI PRGGAGLHKL CREQSTIPVI TGGIGVCHTF VDESADFEKA 250 260 270 280 290 300 LLVIENAKIQ RPSACNSLET LLVHQEIAAR FLPILSDRMH AFGVTLHASP QAMPFLAEGK 310 320 330 340 350 360 ARVVAVEAAD YDDEWLSLDL NVEIVADINA AISHIREHGT SHSDAILTRS LGNAEHFVRA 370 380 390 400 410 VDSSAVYVNA STRFTDGGQF GLGAEVAVST QKLHARGPMG LDALTTYKWI GYGDDLVRS

References

[1]

"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081." Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. , Quail M.A., Dougan G., Parkhill J., Prentice M.B. PLoS Genet. 2:2039-2051(2006) [PubMed: 17173484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 8081.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286415 Genomic DNA. Translation: CAL13233.1.
RefSeq	YP_001007378.1. NC_008800.1.
3D structure databases
ProteinModelPortal	A1JNX6.
ModBase	Search...
Protein-protein interaction databases
STRING	A1JNX6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4715101.
GenomeReviews	Gene locus YE3201 in contig AM286415_GR.
KEGG	yen:YE3201.
NMPDR	fig|630.2.peg.3130.
PATRIC	18566123. VBIYerEnt11519_3404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	QYPAACN.
PhylomeDB	A1JNX6.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	YENT393305:YE3201-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_YERE8

Accession

Primary (citable) accession number: A1JNX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program