ID   FTHS_YERE8              Reviewed;         585 AA.
AC   A1JMA3;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   24-JAN-2024, entry version 83.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=YE1997;
OS   Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 /
OS   8081).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=393305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 13174 / 8081;
RX   PubMed=17173484; DOI=10.1371/journal.pgen.0020206;
RA   Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L.,
RA   Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T.,
RA   Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S.,
RA   Sanders M., Whitehead S., Quail M.A., Dougan G., Parkhill J.,
RA   Prentice M.B.;
RT   "The complete genome sequence and comparative genome analysis of the high
RT   pathogenicity Yersinia enterocolitica strain 8081.";
RL   PLoS Genet. 2:2039-2051(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAL12075.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AM286415; CAL12075.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_042661416.1; NC_008800.1.
DR   RefSeq; YP_001006249.1; NC_008800.1.
DR   AlphaFoldDB; A1JMA3; -.
DR   SMR; A1JMA3; -.
DR   KEGG; yen:YE1997; -.
DR   PATRIC; fig|393305.7.peg.2159; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   OrthoDB; 9761733at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000642; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..585
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000293073"
FT   BINDING         74..81
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   585 AA;  61470 MW;  3822C19C920B3175 CRC64;
     MTPTLSSSAD VLPSLSNMSS ETNLLPIQQI APQLGLSADD LIPYGHHMAK VDISALRPSG
     PQGKLILVSS ITPTPLGEGK TVTTIGLSQG INRLGYRGVA CIRQPSLGPV FGVKGGAAGG
     GAAQVLPMEK LNLHLTGDIH AISAAHNLAA AALDARLYHE QRLGAVFSQQ TGMPLLNIDA
     QQILWPRVVD HNDRALRHIQ VGVGGGTHGV ERHDHVEITA ASELMAILAL SESLHDMRQR
     IGRIILAHST SGQAITADDL GVAGAMTALM KETIHPTLMQ TSEQTPVLIH AGPFANIAHG
     NSSVLADRLG LQLADYVVTE AGFGSDMGME KFFNIKYRQS GITPSCVVLV ATLRSLKANS
     GVFDIKPGQP LPAEILNTNI PLLSQGCANL KWHINNAKSY GLPVVVAVNC FPDDSPEELA
     FLADYALSAG AIACEISEAF AKGGAGTTAL AQRVIDACAH ASPPVLAYPD NASLEQKIEI
     LAQRYGAREV TFTPQARQQL DSITAAGFGH LPLCIAKTPL SISADASLKN VPHDFVLPVT
     ACAVSAGAGF VRIYAGDIMT MPGLGTQPAY YHIDIDDEGC IRGLS
//