A1JMA3 (FTHS_YERE8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	YE1997

Organism

Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) [Complete proteome] [HAMAP]

Taxonomic identifier

393305 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia enterocolitica › Yersinia enterocolitica subsp. enterocolitica

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.
Sequence caution	The sequence CAL12075.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000293073
Regions
	Nucleotide binding	74 – 81	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1JMA3 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 3822C19C920B3175	FASTA	585	61,470
	10 20 30 40 50 60 MTPTLSSSAD VLPSLSNMSS ETNLLPIQQI APQLGLSADD LIPYGHHMAK VDISALRPSG 70 80 90 100 110 120 PQGKLILVSS ITPTPLGEGK TVTTIGLSQG INRLGYRGVA CIRQPSLGPV FGVKGGAAGG 130 140 150 160 170 180 GAAQVLPMEK LNLHLTGDIH AISAAHNLAA AALDARLYHE QRLGAVFSQQ TGMPLLNIDA 190 200 210 220 230 240 QQILWPRVVD HNDRALRHIQ VGVGGGTHGV ERHDHVEITA ASELMAILAL SESLHDMRQR 250 260 270 280 290 300 IGRIILAHST SGQAITADDL GVAGAMTALM KETIHPTLMQ TSEQTPVLIH AGPFANIAHG 310 320 330 340 350 360 NSSVLADRLG LQLADYVVTE AGFGSDMGME KFFNIKYRQS GITPSCVVLV ATLRSLKANS 370 380 390 400 410 420 GVFDIKPGQP LPAEILNTNI PLLSQGCANL KWHINNAKSY GLPVVVAVNC FPDDSPEELA 430 440 450 460 470 480 FLADYALSAG AIACEISEAF AKGGAGTTAL AQRVIDACAH ASPPVLAYPD NASLEQKIEI 490 500 510 520 530 540 LAQRYGAREV TFTPQARQQL DSITAAGFGH LPLCIAKTPL SISADASLKN VPHDFVLPVT 550 560 570 580 ACAVSAGAGF VRIYAGDIMT MPGLGTQPAY YHIDIDDEGC IRGLS

References

[1]

"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081." Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. , Quail M.A., Dougan G., Parkhill J., Prentice M.B. PLoS Genet. 2:2039-2051(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 8081.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286415 Genomic DNA. Translation: CAL12075.1. Different initiation.
RefSeq	YP_001006249.1. NC_008800.1.
3D structure databases
ProteinModelPortal	A1JMA3.
ModBase	Search...
Protein-protein interaction databases
STRING	393305.YE1997.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4715544.
KEGG	yen:YE1997.
PATRIC	18563592. VBIYerEnt11519_2159.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
ProtClustDB	PRK13506.
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. False negative. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_YERE8

Accession

Primary (citable) accession number: A1JMA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program