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A1JL26

- NAPA_YERE8

UniProt

A1JL26 - NAPA_YERE8

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Protein
Periplasmic nitrate reductase
Gene
napA, YE1156
Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S) By similarity
Metal bindingi82 – 821Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:YE1156
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000642: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signal Reviewed prediction
Add
BLAST
Chaini32 – 831800Periplasmic nitrate reductaseUniRule annotation
PRO_5000201017Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi393305.YE1156.

Structurei

3D structure databases

ProteinModelPortaliA1JL26.
SMRiA1JL26. Positions 39-828.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1JL26-1 [UniParc]FASTAAdd to Basket

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MKLSRRDFMK ANAAVAAAAA AGLTIPTVAK AVVGETTNAI KWDKAPCRFC    50
GTGCGVLVGT QNGRIVASQG DPDSPVNRGL NCVKGYFLPK IMYGKDRLTQ 100
PLLRMKDGQY DKEGDFTPIS WEKAFDIMEL KFKNALKEKG PTAVGMFGSG 150
QWTVWEGYAA SKLLKAGFRS NNLDPNARHC MASSVVGFMR TFGMDEPMGC 200
YDDIEEADAF VLWGSNMAEM HPILWSRMTS RRLTNEHVRI AVLSTFEHRS 250
FELADNPIVF TPQTDLVIMN YIANYIIQNN AVDQGFLDRH VNFRRGATDI 300
GYGLRPTHPL EKAAKNPGSD ASEPMSFDDF KAFVAEYTLE KTAKMSGVPE 350
DQLESLAQLY ADPKVKLVSY WTMGFNQHTR GVWANNMCYN LHLLTGKISK 400
PGSGPFSLTG QPSACGTARE VGTFSHRLPA DMVVTNEKHR QIAETKWQLP 450
AGTIPEKVGL HAVAQDRALK DGTLNAYWVM CNNNMQAGPN INEERMPGWR 500
DPRNFIVVSD PYPTISALAA DLILPTAMWV EKEGAYGNAE RRTQFWRQQV 550
PAPGEAKSDL WQMVEFAKRF KVEEVWPDEL INKKPEYRGK TLYDVLFANN 600
VVNKYPLSEI PADQLNDEAR DFGFYIQKGL FEEYADFGRG HGHDLAPFDR 650
YHQERGLRWP VVNGKETLWR YREGFDPFVP KGEDVRFYGK PDGKAVIFAL 700
PYEPAAESPD QEYDLWLSTG RVLEHWHTGS MTRRVPELHR AFPEAVLFIH 750
PLDAKARGLR RGDKVKVISR RGEVISLVET RGRNRPPQGL VYMPFFDAAQ 800
LVNNLTLDAT DPLSKETDFK KCAVKLARVV A 831
Length:831
Mass (Da):93,407
Last modified:February 6, 2007 - v1
Checksum:iC7CFFE972D7248D0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM286415 Genomic DNA. Translation: CAL11250.1.
RefSeqiWP_011815828.1. NC_008800.1.
YP_001005483.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL11250; CAL11250; YE1156.
GeneIDi4712672.
KEGGiyen:YE1156.
PATRICi18561764. VBIYerEnt11519_1262.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM286415 Genomic DNA. Translation: CAL11250.1 .
RefSeqi WP_011815828.1. NC_008800.1.
YP_001005483.1. NC_008800.1.

3D structure databases

ProteinModelPortali A1JL26.
SMRi A1JL26. Positions 39-828.
ModBasei Search...

Protein-protein interaction databases

STRINGi 393305.YE1156.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL11250 ; CAL11250 ; YE1156 .
GeneIDi 4712672.
KEGGi yen:YE1156.
PATRICi 18561764. VBIYerEnt11519_1262.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 13174 / 8081.

Entry informationi

Entry nameiNAPA_YERE8
AccessioniPrimary (citable) accession number: A1JL26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: September 3, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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