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A1JL26 (NAPA_YERE8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:YE1156
OrganismYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) [Complete proteome] [HAMAP]
Taxonomic identifier393305 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 831800Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000201017

Regions

Domain40 – 96574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A1JL26 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: C7CFFE972D7248D0

FASTA83193,407
        10         20         30         40         50         60 
MKLSRRDFMK ANAAVAAAAA AGLTIPTVAK AVVGETTNAI KWDKAPCRFC GTGCGVLVGT 

        70         80         90        100        110        120 
QNGRIVASQG DPDSPVNRGL NCVKGYFLPK IMYGKDRLTQ PLLRMKDGQY DKEGDFTPIS 

       130        140        150        160        170        180 
WEKAFDIMEL KFKNALKEKG PTAVGMFGSG QWTVWEGYAA SKLLKAGFRS NNLDPNARHC 

       190        200        210        220        230        240 
MASSVVGFMR TFGMDEPMGC YDDIEEADAF VLWGSNMAEM HPILWSRMTS RRLTNEHVRI 

       250        260        270        280        290        300 
AVLSTFEHRS FELADNPIVF TPQTDLVIMN YIANYIIQNN AVDQGFLDRH VNFRRGATDI 

       310        320        330        340        350        360 
GYGLRPTHPL EKAAKNPGSD ASEPMSFDDF KAFVAEYTLE KTAKMSGVPE DQLESLAQLY 

       370        380        390        400        410        420 
ADPKVKLVSY WTMGFNQHTR GVWANNMCYN LHLLTGKISK PGSGPFSLTG QPSACGTARE 

       430        440        450        460        470        480 
VGTFSHRLPA DMVVTNEKHR QIAETKWQLP AGTIPEKVGL HAVAQDRALK DGTLNAYWVM 

       490        500        510        520        530        540 
CNNNMQAGPN INEERMPGWR DPRNFIVVSD PYPTISALAA DLILPTAMWV EKEGAYGNAE 

       550        560        570        580        590        600 
RRTQFWRQQV PAPGEAKSDL WQMVEFAKRF KVEEVWPDEL INKKPEYRGK TLYDVLFANN 

       610        620        630        640        650        660 
VVNKYPLSEI PADQLNDEAR DFGFYIQKGL FEEYADFGRG HGHDLAPFDR YHQERGLRWP 

       670        680        690        700        710        720 
VVNGKETLWR YREGFDPFVP KGEDVRFYGK PDGKAVIFAL PYEPAAESPD QEYDLWLSTG 

       730        740        750        760        770        780 
RVLEHWHTGS MTRRVPELHR AFPEAVLFIH PLDAKARGLR RGDKVKVISR RGEVISLVET 

       790        800        810        820        830 
RGRNRPPQGL VYMPFFDAAQ LVNNLTLDAT DPLSKETDFK KCAVKLARVV A 

« Hide

References

[1]"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081."
Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. expand/collapse author list , Quail M.A., Dougan G., Parkhill J., Prentice M.B.
PLoS Genet. 2:2039-2051(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 13174 / 8081.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM286415 Genomic DNA. Translation: CAL11250.1.
RefSeqYP_001005483.1. NC_008800.1.

3D structure databases

ProteinModelPortalA1JL26.
SMRA1JL26. Positions 39-828.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING393305.YE1156.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL11250; CAL11250; YE1156.
GeneID4712672.
KEGGyen:YE1156.
PATRIC18561764. VBIYerEnt11519_1262.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAVNNGLAC.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_YERE8
AccessionPrimary (citable) accession number: A1JL26
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families