UniProtKB - A1JL26 (NAPA_YERE8)
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Protein
Periplasmic nitrate reductase
Gene
napA
Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Functioni
Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation
Catalytic activityi
2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation
Cofactori
Protein has several cofactor binding sites:- [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
- Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 50 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 54 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 82 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Binding sitei | 84 | Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation | 1 | |
Binding sitei | 151 | Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation | 1 | |
Binding sitei | 176 | Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation | 1 | |
Binding sitei | 180 | Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation | 1 | |
Binding sitei | 373 | Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 377 | Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation | 1 | |
Binding sitei | 483 | Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 532 | Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation | 1 | |
Binding sitei | 559 | Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation | 1 | |
Binding sitei | 795 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 803 | Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation | 1 | |
Binding sitei | 820 | Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- molybdenum ion binding Source: InterPro
- molybdopterin cofactor binding Source: InterPro
- nitrate reductase (cytochrome) activity Source: UniProtKB-EC
- nitrate reductase activity Source: InterPro
GO - Biological processi
- nitrate assimilation Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Nitrate assimilation, Transport |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:napAUniRule annotation Ordered Locus Names:YE1156 |
Organismi | Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) |
Taxonomic identifieri | 393305 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Yersiniaceae › Yersinia › |
Proteomesi |
|
Subcellular locationi
- Periplasm UniRule annotation
GO - Cellular componenti
- periplasmic space Source: UniProtKB-SubCell
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Tat-type signalUniRule annotationAdd BLAST | 31 | |
ChainiPRO_5000201017 | 32 – 831 | Periplasmic nitrate reductaseUniRule annotationAdd BLAST | 800 |
Post-translational modificationi
Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation
Interactioni
Subunit structurei
Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation
Protein-protein interaction databases
STRINGi | 393305.YE1156. |
Structurei
3D structure databases
ProteinModelPortali | A1JL26. |
SMRi | A1JL26. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 40 – 96 | 4Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST | 57 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 213 – 220 | Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation | 8 | |
Regioni | 244 – 248 | Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation | 5 | |
Regioni | 263 – 265 | Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation | 3 | |
Regioni | 509 – 510 | Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation | 2 | |
Regioni | 719 – 728 | Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation | 10 |
Sequence similaritiesi
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG4107QIW. Bacteria. COG0243. LUCA. |
HOGENOMi | HOG000031441. |
KOi | K02567. |
OMAi | ANSMGTR. |
OrthoDBi | POG091H060P. |
Family and domain databases
HAMAPi | MF_01630. Nitrate_reduct_NapA. 1 hit. |
InterProi | View protein in InterPro IPR009010. Asp_de-COase-like_dom_sf. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_4Fe-4S_dom. IPR027467. MopterinOxRdtase_cofactor_BS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. |
PANTHERi | PTHR11615:SF123. PTHR11615:SF123. 1 hit. |
Pfami | View protein in Pfam PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. PF10518. TAT_signal. 1 hit. |
SMARTi | View protein in SMART SM00926. Molybdop_Fe4S4. 1 hit. |
SUPFAMi | SSF50692. SSF50692. 1 hit. |
TIGRFAMsi | TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. |
PROSITEi | View protein in PROSITE PS51669. 4FE4S_MOW_BIS_MGD. 1 hit. PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS51318. TAT. 1 hit. |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A1JL26-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLSRRDFMK ANAAVAAAAA AGLTIPTVAK AVVGETTNAI KWDKAPCRFC
60 70 80 90 100
GTGCGVLVGT QNGRIVASQG DPDSPVNRGL NCVKGYFLPK IMYGKDRLTQ
110 120 130 140 150
PLLRMKDGQY DKEGDFTPIS WEKAFDIMEL KFKNALKEKG PTAVGMFGSG
160 170 180 190 200
QWTVWEGYAA SKLLKAGFRS NNLDPNARHC MASSVVGFMR TFGMDEPMGC
210 220 230 240 250
YDDIEEADAF VLWGSNMAEM HPILWSRMTS RRLTNEHVRI AVLSTFEHRS
260 270 280 290 300
FELADNPIVF TPQTDLVIMN YIANYIIQNN AVDQGFLDRH VNFRRGATDI
310 320 330 340 350
GYGLRPTHPL EKAAKNPGSD ASEPMSFDDF KAFVAEYTLE KTAKMSGVPE
360 370 380 390 400
DQLESLAQLY ADPKVKLVSY WTMGFNQHTR GVWANNMCYN LHLLTGKISK
410 420 430 440 450
PGSGPFSLTG QPSACGTARE VGTFSHRLPA DMVVTNEKHR QIAETKWQLP
460 470 480 490 500
AGTIPEKVGL HAVAQDRALK DGTLNAYWVM CNNNMQAGPN INEERMPGWR
510 520 530 540 550
DPRNFIVVSD PYPTISALAA DLILPTAMWV EKEGAYGNAE RRTQFWRQQV
560 570 580 590 600
PAPGEAKSDL WQMVEFAKRF KVEEVWPDEL INKKPEYRGK TLYDVLFANN
610 620 630 640 650
VVNKYPLSEI PADQLNDEAR DFGFYIQKGL FEEYADFGRG HGHDLAPFDR
660 670 680 690 700
YHQERGLRWP VVNGKETLWR YREGFDPFVP KGEDVRFYGK PDGKAVIFAL
710 720 730 740 750
PYEPAAESPD QEYDLWLSTG RVLEHWHTGS MTRRVPELHR AFPEAVLFIH
760 770 780 790 800
PLDAKARGLR RGDKVKVISR RGEVISLVET RGRNRPPQGL VYMPFFDAAQ
810 820 830
LVNNLTLDAT DPLSKETDFK KCAVKLARVV A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM286415 Genomic DNA. Translation: CAL11250.1. |
RefSeqi | WP_011815828.1. NC_008800.1. YP_001005483.1. NC_008800.1. |
Genome annotation databases
EnsemblBacteriai | CAL11250; CAL11250; YE1156. |
GeneIDi | 4712672. |
KEGGi | yen:YE1156. |
PATRICi | fig|393305.7.peg.1262. |
Similar proteinsi
Entry informationi
Entry namei | NAPA_YERE8 | |
Accessioni | A1JL26Primary (citable) accession number: A1JL26 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | February 6, 2007 | |
Last modified: | March 28, 2018 | |
This is version 82 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |