Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A1JL26

- NAPA_YERE8

UniProt

A1JL26 - NAPA_YERE8

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:YE1156
    OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
    Taxonomic identifieri393305 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000642: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
    BLAST
    Chaini32 – 831800Periplasmic nitrate reductasePRO_5000201017Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi393305.YE1156.

    Structurei

    3D structure databases

    ProteinModelPortaliA1JL26.
    SMRiA1JL26. Positions 39-828.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1JL26-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLSRRDFMK ANAAVAAAAA AGLTIPTVAK AVVGETTNAI KWDKAPCRFC    50
    GTGCGVLVGT QNGRIVASQG DPDSPVNRGL NCVKGYFLPK IMYGKDRLTQ 100
    PLLRMKDGQY DKEGDFTPIS WEKAFDIMEL KFKNALKEKG PTAVGMFGSG 150
    QWTVWEGYAA SKLLKAGFRS NNLDPNARHC MASSVVGFMR TFGMDEPMGC 200
    YDDIEEADAF VLWGSNMAEM HPILWSRMTS RRLTNEHVRI AVLSTFEHRS 250
    FELADNPIVF TPQTDLVIMN YIANYIIQNN AVDQGFLDRH VNFRRGATDI 300
    GYGLRPTHPL EKAAKNPGSD ASEPMSFDDF KAFVAEYTLE KTAKMSGVPE 350
    DQLESLAQLY ADPKVKLVSY WTMGFNQHTR GVWANNMCYN LHLLTGKISK 400
    PGSGPFSLTG QPSACGTARE VGTFSHRLPA DMVVTNEKHR QIAETKWQLP 450
    AGTIPEKVGL HAVAQDRALK DGTLNAYWVM CNNNMQAGPN INEERMPGWR 500
    DPRNFIVVSD PYPTISALAA DLILPTAMWV EKEGAYGNAE RRTQFWRQQV 550
    PAPGEAKSDL WQMVEFAKRF KVEEVWPDEL INKKPEYRGK TLYDVLFANN 600
    VVNKYPLSEI PADQLNDEAR DFGFYIQKGL FEEYADFGRG HGHDLAPFDR 650
    YHQERGLRWP VVNGKETLWR YREGFDPFVP KGEDVRFYGK PDGKAVIFAL 700
    PYEPAAESPD QEYDLWLSTG RVLEHWHTGS MTRRVPELHR AFPEAVLFIH 750
    PLDAKARGLR RGDKVKVISR RGEVISLVET RGRNRPPQGL VYMPFFDAAQ 800
    LVNNLTLDAT DPLSKETDFK KCAVKLARVV A 831
    Length:831
    Mass (Da):93,407
    Last modified:February 6, 2007 - v1
    Checksum:iC7CFFE972D7248D0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM286415 Genomic DNA. Translation: CAL11250.1.
    RefSeqiWP_011815828.1. NC_008800.1.
    YP_001005483.1. NC_008800.1.

    Genome annotation databases

    EnsemblBacteriaiCAL11250; CAL11250; YE1156.
    GeneIDi4712672.
    KEGGiyen:YE1156.
    PATRICi18561764. VBIYerEnt11519_1262.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM286415 Genomic DNA. Translation: CAL11250.1 .
    RefSeqi WP_011815828.1. NC_008800.1.
    YP_001005483.1. NC_008800.1.

    3D structure databases

    ProteinModelPortali A1JL26.
    SMRi A1JL26. Positions 39-828.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 393305.YE1156.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL11250 ; CAL11250 ; YE1156 .
    GeneIDi 4712672.
    KEGGi yen:YE1156.
    PATRICi 18561764. VBIYerEnt11519_1262.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 13174 / 8081.

    Entry informationi

    Entry nameiNAPA_YERE8
    AccessioniPrimary (citable) accession number: A1JL26
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3