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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi50Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi54Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei84Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei151Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei176Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei180Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei373Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei377Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei483Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei532Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei559Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei795Substrate; via amide nitrogenUniRule annotation1
Binding sitei803Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei820Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:YE1156
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000642 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalUniRule annotationAdd BLAST31
ChainiPRO_500020101732 – 831Periplasmic nitrate reductaseUniRule annotationAdd BLAST800

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi393305.YE1156.

Structurei

3D structure databases

ProteinModelPortaliA1JL26.
SMRiA1JL26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 964Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 220Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni244 – 248Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni263 – 265Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni509 – 510Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni719 – 728Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000031441.
KOiK02567.
OMAiANSMGTR.
OrthoDBiPOG091H060P.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct_NapA. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom_sf.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
PANTHERiPTHR11615:SF123. PTHR11615:SF123. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1JL26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSRRDFMK ANAAVAAAAA AGLTIPTVAK AVVGETTNAI KWDKAPCRFC
60 70 80 90 100
GTGCGVLVGT QNGRIVASQG DPDSPVNRGL NCVKGYFLPK IMYGKDRLTQ
110 120 130 140 150
PLLRMKDGQY DKEGDFTPIS WEKAFDIMEL KFKNALKEKG PTAVGMFGSG
160 170 180 190 200
QWTVWEGYAA SKLLKAGFRS NNLDPNARHC MASSVVGFMR TFGMDEPMGC
210 220 230 240 250
YDDIEEADAF VLWGSNMAEM HPILWSRMTS RRLTNEHVRI AVLSTFEHRS
260 270 280 290 300
FELADNPIVF TPQTDLVIMN YIANYIIQNN AVDQGFLDRH VNFRRGATDI
310 320 330 340 350
GYGLRPTHPL EKAAKNPGSD ASEPMSFDDF KAFVAEYTLE KTAKMSGVPE
360 370 380 390 400
DQLESLAQLY ADPKVKLVSY WTMGFNQHTR GVWANNMCYN LHLLTGKISK
410 420 430 440 450
PGSGPFSLTG QPSACGTARE VGTFSHRLPA DMVVTNEKHR QIAETKWQLP
460 470 480 490 500
AGTIPEKVGL HAVAQDRALK DGTLNAYWVM CNNNMQAGPN INEERMPGWR
510 520 530 540 550
DPRNFIVVSD PYPTISALAA DLILPTAMWV EKEGAYGNAE RRTQFWRQQV
560 570 580 590 600
PAPGEAKSDL WQMVEFAKRF KVEEVWPDEL INKKPEYRGK TLYDVLFANN
610 620 630 640 650
VVNKYPLSEI PADQLNDEAR DFGFYIQKGL FEEYADFGRG HGHDLAPFDR
660 670 680 690 700
YHQERGLRWP VVNGKETLWR YREGFDPFVP KGEDVRFYGK PDGKAVIFAL
710 720 730 740 750
PYEPAAESPD QEYDLWLSTG RVLEHWHTGS MTRRVPELHR AFPEAVLFIH
760 770 780 790 800
PLDAKARGLR RGDKVKVISR RGEVISLVET RGRNRPPQGL VYMPFFDAAQ
810 820 830
LVNNLTLDAT DPLSKETDFK KCAVKLARVV A
Length:831
Mass (Da):93,407
Last modified:February 6, 2007 - v1
Checksum:iC7CFFE972D7248D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL11250.1.
RefSeqiWP_011815828.1. NC_008800.1.
YP_001005483.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL11250; CAL11250; YE1156.
GeneIDi4712672.
KEGGiyen:YE1156.
PATRICifig|393305.7.peg.1262.

Similar proteinsi

Entry informationi

Entry nameiNAPA_YERE8
AccessioniPrimary (citable) accession number: A1JL26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: March 28, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome