A1JL26 (NAPA_YERE8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase
EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	YE1156

Organism

Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) [Complete proteome] [HAMAP]

Taxonomic identifier

393305 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia enterocolitica › Yersinia enterocolitica subsp. enterocolitica

Protein attributes

Sequence length	831 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630 Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.
Subunit structure	Interacts with NapB By similarity. HAMAP-Rule MF_01630
Subcellular location	Periplasm By similarity HAMAP-Rule MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily. Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-HAMAP nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-HAMAP electron carrier activity Inferred from electronic annotation. Source: UniProtKB-HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Tat-type signal Potential
	Chain	32 – 831	800	Periplasmic nitrate reductase HAMAP-Rule MF_01630		PRO_5000201017
Regions
	Domain	40 – 96	57	4Fe-4S Mo/W bis-MGD-type
Sites
	Metal binding	47	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	50	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	54	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	82	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1JL26 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C7CFFE972D7248D0	FASTA	831	93,407
	10 20 30 40 50 60 MKLSRRDFMK ANAAVAAAAA AGLTIPTVAK AVVGETTNAI KWDKAPCRFC GTGCGVLVGT 70 80 90 100 110 120 QNGRIVASQG DPDSPVNRGL NCVKGYFLPK IMYGKDRLTQ PLLRMKDGQY DKEGDFTPIS 130 140 150 160 170 180 WEKAFDIMEL KFKNALKEKG PTAVGMFGSG QWTVWEGYAA SKLLKAGFRS NNLDPNARHC 190 200 210 220 230 240 MASSVVGFMR TFGMDEPMGC YDDIEEADAF VLWGSNMAEM HPILWSRMTS RRLTNEHVRI 250 260 270 280 290 300 AVLSTFEHRS FELADNPIVF TPQTDLVIMN YIANYIIQNN AVDQGFLDRH VNFRRGATDI 310 320 330 340 350 360 GYGLRPTHPL EKAAKNPGSD ASEPMSFDDF KAFVAEYTLE KTAKMSGVPE DQLESLAQLY 370 380 390 400 410 420 ADPKVKLVSY WTMGFNQHTR GVWANNMCYN LHLLTGKISK PGSGPFSLTG QPSACGTARE 430 440 450 460 470 480 VGTFSHRLPA DMVVTNEKHR QIAETKWQLP AGTIPEKVGL HAVAQDRALK DGTLNAYWVM 490 500 510 520 530 540 CNNNMQAGPN INEERMPGWR DPRNFIVVSD PYPTISALAA DLILPTAMWV EKEGAYGNAE 550 560 570 580 590 600 RRTQFWRQQV PAPGEAKSDL WQMVEFAKRF KVEEVWPDEL INKKPEYRGK TLYDVLFANN 610 620 630 640 650 660 VVNKYPLSEI PADQLNDEAR DFGFYIQKGL FEEYADFGRG HGHDLAPFDR YHQERGLRWP 670 680 690 700 710 720 VVNGKETLWR YREGFDPFVP KGEDVRFYGK PDGKAVIFAL PYEPAAESPD QEYDLWLSTG 730 740 750 760 770 780 RVLEHWHTGS MTRRVPELHR AFPEAVLFIH PLDAKARGLR RGDKVKVISR RGEVISLVET 790 800 810 820 830 RGRNRPPQGL VYMPFFDAAQ LVNNLTLDAT DPLSKETDFK KCAVKLARVV A

References

[1]

"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081." Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. , Quail M.A., Dougan G., Parkhill J., Prentice M.B. PLoS Genet. 2:2039-2051(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 13174 / 8081.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286415 Genomic DNA. Translation: CAL11250.1.
RefSeq	YP_001005483.1. NC_008800.1.
3D structure databases
ProteinModelPortal	A1JL26.
SMR	A1JL26. Positions 39-828.
ModBase	Search...
Protein-protein interaction databases
STRING	393305.YE1156.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL11250; CAL11250; YE1156.
GeneID	4712672.
KEGG	yen:YE1156.
PATRIC	18561764. VBIYerEnt11519_1262.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031441.
KO	K02567.
OMA	VNNGLAC.
OrthoDB	EOG6CVV7G.
ProtClustDB	PRK13532.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_4Fe-4S_dom. IPR027467. MopterinOxRdtase_cofactor_BS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. SSF50692. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51669. 4FE4S_MOW_BIS_MGD. 1 hit. PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_YERE8

Accession

Primary (citable) accession number: A1JL26

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	November 13, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program