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A1JK30

- FADJ_YERE8

UniProt

A1JK30 - FADJ_YERE8

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei137 – 1371Important for catalytic activityUniRule annotation
    Sitei159 – 1591Important for catalytic activityUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
    3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
    Gene namesi
    Name:fadJUniRule annotation
    Synonyms:faoA
    Ordered Locus Names:YE1277
    OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
    Taxonomic identifieri393305 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000642: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 745745Fatty acid oxidation complex subunit alphaPRO_0000323532Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

    Protein-protein interaction databases

    STRINGi393305.YE1277.

    Structurei

    3D structure databases

    ProteinModelPortaliA1JK30.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni47 – 209163Enoyl-CoA hydrataseUniRule annotationAdd
    BLAST
    Regioni325 – 7454213-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261346.
    KOiK01782.
    OrthoDBiEOG6M9F0M.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPiMF_01617. FadJ.
    InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02440. FadJ. 1 hit.
    PROSITEiPS00067. 3HCDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1JK30-1 [UniParc]FASTAAdd to Basket

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    MSQENTLNSG DETILAEAVA PAHAVFTLSV RPDNIGIITV DVVGDKVNTL    50
    KAKFAEQIAE ILQQAQALSQ LQGLVIISGK PDSFIAGADI TMIAACHTAQ 100
    DARILAQKGQ SILAQIAAFP VPVVAAIHGA CLGGGLELAL ACHSRICSQD 150
    DKTVLGLPEV QLGLLPGSGG TQRLPRLVGV SKALDMILTG RQVRARQALK 200
    MGLVDDVVPQ DILLDVAIQR AKAGWLDKPA LPWQERLLSG PLGKALLFNI 250
    VRKKTQAKTK GHYPAAERII DVVRKGLDHG GPAGYEAEAK AFGELAMTPE 300
    SAALRSLFFA TTSLKKESGG KAQPRAIHRV GVLGGGLMGG GIANVTATRA 350
    GLPVRIKDIN PTGINQALKY TWDTLGKRVR SKRMRPAERQ RQMMLISGST 400
    DYCGFGNVDI VVEAVFEDLS LKQQMVADIE HFAAPHTIFA SNTSSLPISD 450
    IAAQAQRPEQ VIGLHYFSPV DKMPLVEVIP HAKTSEETIA TTVALARKQG 500
    KTAIVVADRA GFYVNRILAP YINEAARCLL DGEPIASVDK ALVDFGFPVG 550
    PITLLDEVGI DVGTKIIPIL VEKLGARFAA PPSFDVILKD GRKGRKNGRG 600
    FYLYPAKSSG FKWKRSPVKQ VDTSVYTLLG VTPKAHLESA VIAQRCTMMM 650
    LNEAARCLDE SIIRNPRDGD IGAVFGIGFP PFLGGPFRYM DSLGADKVVK 700
    TLNLLAQQYG ERFEPCSLLV TMAGQQKRFY PPENSLDEAA ITAHN 745
    Length:745
    Mass (Da):79,924
    Last modified:March 18, 2008 - v2
    Checksum:iB31E7B68E3F51798
    GO

    Sequence cautioni

    The sequence CAL11369.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM286415 Genomic DNA. Translation: CAL11369.1. Different initiation.
    RefSeqiYP_001005598.1. NC_008800.1.

    Genome annotation databases

    EnsemblBacteriaiCAL11369; CAL11369; YE1277.
    GeneIDi4715636.
    KEGGiyen:YE1277.
    PATRICi18562028. VBIYerEnt11519_1387.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM286415 Genomic DNA. Translation: CAL11369.1 . Different initiation.
    RefSeqi YP_001005598.1. NC_008800.1.

    3D structure databases

    ProteinModelPortali A1JK30.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 393305.YE1277.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL11369 ; CAL11369 ; YE1277 .
    GeneIDi 4715636.
    KEGGi yen:YE1277.
    PATRICi 18562028. VBIYerEnt11519_1387.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261346.
    KOi K01782.
    OrthoDBi EOG6M9F0M.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPi MF_01617. FadJ.
    InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
    IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02440. FadJ. 1 hit.
    PROSITEi PS00067. 3HCDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 13174 / 8081.

    Entry informationi

    Entry nameiFADJ_YERE8
    AccessioniPrimary (citable) accession number: A1JK30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 59 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3