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Protein

Sulfate adenylyltransferase subunit 1

Gene

cysN

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May be the GTPase, regulating ATP sulfurylase activity.UniRule annotation

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 2 (cysD), Sulfate adenylyltransferase subunit 1 (cysN)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 44GTPUniRule annotation8
Nucleotide bindingi116 – 120GTPUniRule annotation5
Nucleotide bindingi171 – 174GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase large subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysNUniRule annotation
Ordered Locus Names:YE0763
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000642 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000921651 – 478Sulfate adenylyltransferase subunit 1Add BLAST478

Proteomic databases

PRIDEiA1JJT0.

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Protein-protein interaction databases

STRINGi393305.YE0763.

Structurei

3D structure databases

ProteinModelPortaliA1JJT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 244tr-type GAdd BLAST217

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 44G1By similarity8
Regioni95 – 99G2By similarity5
Regioni116 – 119G3By similarity4
Regioni171 – 174G4By similarity4
Regioni209 – 211G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00956.
OMAiASIDVVW.
OrthoDBiPOG091H06C6.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1JJT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILQNNSIAQ QIANEGGVEA YLHAQQHKTM LRFLTCGSVD DGKSTLIGRL
60 70 80 90 100
LHDTRQIYED QLTTLHTDSK RLGTQGEKLD LALLVDGLQA EREQGITIDV
110 120 130 140 150
AYRYFSTEQR KFIIADTPGH EQYTRNMATG ASTCDLAILL IDARKGVLDQ
160 170 180 190 200
TRRHSFIATL LGIRHLVVAV NKMDLVDYQE SVFEQFKQDY LSFAQQLPTD
210 220 230 240 250
LDIKFVPLSA LDGDNVASPS EKMNWYSGPT LLEVLESVDV VNASRQQPLR
260 270 280 290 300
FPVQYVNRPN LDFRGYAGTL SAGIVRVGQK IKVLPSGVES TVARIVTFDG
310 320 330 340 350
DLTQAGPGEA ITLVLTDEVD ISRGDLLVDA SETLQAAQNA LVDVVWMAEQ
360 370 380 390 400
PLVAGQSYDI KIAGKKTRAR VENIQYQVEI NSLTQRVVES LPLNGIGLVE
410 420 430 440 450
LAFDEPLVLD SYQRNRDTGG MIFIDRLSNV TVGAGLIRET LESVYQENTE
460 470
FSAFELELNA LVRRHFPHWG ARDLLGGK
Length:478
Mass (Da):53,050
Last modified:February 6, 2007 - v1
Checksum:iA43F3FAD9D5CA32F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL10867.1.
RefSeqiWP_011815608.1. NC_008800.1.
YP_001005106.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL10867; CAL10867; YE0763.
GeneIDi4716115.
KEGGiyen:YE0763.
PATRICi18560936. VBIYerEnt11519_0857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL10867.1.
RefSeqiWP_011815608.1. NC_008800.1.
YP_001005106.1. NC_008800.1.

3D structure databases

ProteinModelPortaliA1JJT0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi393305.YE0763.

Proteomic databases

PRIDEiA1JJT0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL10867; CAL10867; YE0763.
GeneIDi4716115.
KEGGiyen:YE0763.
PATRICi18560936. VBIYerEnt11519_0857.

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00956.
OMAiASIDVVW.
OrthoDBiPOG091H06C6.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSN_YERE8
AccessioniPrimary (citable) accession number: A1JJT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.