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Protein

Sulfate adenylyltransferase subunit 1

Gene

cysN

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May be the GTPase, regulating ATP sulfurylase activity.UniRule annotation

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 2 (cysD), Sulfate adenylyltransferase subunit 1 (cysN)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 448GTPUniRule annotation
Nucleotide bindingi116 – 1205GTPUniRule annotation
Nucleotide bindingi171 – 1744GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase large subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysNUniRule annotation
Ordered Locus Names:YE0763
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000000642 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Sulfate adenylyltransferase subunit 1PRO_1000092165Add
BLAST

Proteomic databases

PRIDEiA1JJT0.

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Protein-protein interaction databases

STRINGi393305.YE0763.

Structurei

3D structure databases

ProteinModelPortaliA1JJT0.
SMRiA1JJT0. Positions 23-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 244217tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 448G1By similarity
Regioni95 – 995G2By similarity
Regioni116 – 1194G3By similarity
Regioni171 – 1744G4By similarity
Regioni209 – 2113G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00956.
OMAiIQDVYKF.
OrthoDBiEOG6GR38Q.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1JJT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILQNNSIAQ QIANEGGVEA YLHAQQHKTM LRFLTCGSVD DGKSTLIGRL
60 70 80 90 100
LHDTRQIYED QLTTLHTDSK RLGTQGEKLD LALLVDGLQA EREQGITIDV
110 120 130 140 150
AYRYFSTEQR KFIIADTPGH EQYTRNMATG ASTCDLAILL IDARKGVLDQ
160 170 180 190 200
TRRHSFIATL LGIRHLVVAV NKMDLVDYQE SVFEQFKQDY LSFAQQLPTD
210 220 230 240 250
LDIKFVPLSA LDGDNVASPS EKMNWYSGPT LLEVLESVDV VNASRQQPLR
260 270 280 290 300
FPVQYVNRPN LDFRGYAGTL SAGIVRVGQK IKVLPSGVES TVARIVTFDG
310 320 330 340 350
DLTQAGPGEA ITLVLTDEVD ISRGDLLVDA SETLQAAQNA LVDVVWMAEQ
360 370 380 390 400
PLVAGQSYDI KIAGKKTRAR VENIQYQVEI NSLTQRVVES LPLNGIGLVE
410 420 430 440 450
LAFDEPLVLD SYQRNRDTGG MIFIDRLSNV TVGAGLIRET LESVYQENTE
460 470
FSAFELELNA LVRRHFPHWG ARDLLGGK
Length:478
Mass (Da):53,050
Last modified:February 6, 2007 - v1
Checksum:iA43F3FAD9D5CA32F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL10867.1.
RefSeqiWP_011815608.1. NC_008800.1.
YP_001005106.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL10867; CAL10867; YE0763.
GeneIDi4716115.
KEGGiyen:YE0763.
PATRICi18560936. VBIYerEnt11519_0857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL10867.1.
RefSeqiWP_011815608.1. NC_008800.1.
YP_001005106.1. NC_008800.1.

3D structure databases

ProteinModelPortaliA1JJT0.
SMRiA1JJT0. Positions 23-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi393305.YE0763.

Proteomic databases

PRIDEiA1JJT0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL10867; CAL10867; YE0763.
GeneIDi4716115.
KEGGiyen:YE0763.
PATRICi18560936. VBIYerEnt11519_0857.

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00956.
OMAiIQDVYKF.
OrthoDBiEOG6GR38Q.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 13174 / 8081.

Entry informationi

Entry nameiCYSN_YERE8
AccessioniPrimary (citable) accession number: A1JJT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 6, 2007
Last modified: November 11, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.