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A1IGV8

- AAGAR_THASX

UniProt

A1IGV8 - AAGAR_THASX

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Protein

Alpha-agarase

Gene

agaA33

Organism
Thalassotalea agarivorans (Thalassomonas agarivorans)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Alpha-agarase. Hydrolyzes agarose, agarohexaose, neoagarohexaose and porphyran. Hydrolysis of porphyran by this enzyme improves its antioxidant activity. Does not hydrolyze kappa-carrageenan, iota-carrageenen or lambda-carrageenan.2 Publications

Catalytic activityi

Endohydrolysis of 1,3-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product.2 Publications

Cofactori

Calcium.1 Publication

pH dependencei

Optimum pH is 8.5. Active between pH 4.5 and 9.5, stable between pH 6.5 and 10.5.2 Publications

Temperature dependencei

Optimum temperature is 45 degrees Celsius. Stable up to 40 degrees Celsius.2 Publications

GO - Molecular functioni

  1. alpha-agarase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro
  3. carbohydrate binding Source: InterPro

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16654.
BRENDAi3.2.1.158. 8322.

Protein family/group databases

CAZyiCBM6. Carbohydrate-Binding Module Family 6.
GH96. Glycoside Hydrolase Family 96.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-agarase1 Publication (EC:3.2.1.158)
Alternative name(s):
AgaraseA331 Publication
Gene namesi
Name:agaA33Imported
OrganismiThalassotalea agarivorans (Thalassomonas agarivorans)
Taxonomic identifieri349064 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeThalassotalea

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 6846571 PublicationSequence AnalysisPRO_0000397928Add
BLAST
Chaini685 – 1463779Alpha-agarase1 PublicationPRO_0000397929Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliA1IGV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini701 – 832132CBM6PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 96 family.By similarity
Contains 1 CBM6 (carbohydrate binding type-6) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 3 hits.
3.90.182.10. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR011658. PA14.
IPR003367. Thrombospondin_3-like_rpt.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF03422. CBM_6. 1 hit.
PF02412. TSP_3. 6 hits.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 2 hits.
SSF49785. SSF49785. 3 hits.
PROSITEiPS51175. CBM6. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1IGV8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITSSKKIVS AMLSTSLWIG VASAAYAETT NVEAEGYSTI GGTYQDGNPQ
60 70 80 90 100
PINIYSVNGV QAINFVNRGD FAEYDVSVST AGEYSIEYLI GTSIASGSAV
110 120 130 140 150
EISVLVDGNW QSAGSTNVPL GQWDNFQALA ANNNISLAQG TNRIKITGAG
160 170 180 190 200
THDWQWNLDA FSLTLVTPEN PDNPDNPDNP DDGNTGQPGT PFTIEMEAFD
210 220 230 240 250
ATGSDDPRAQ GMVIGERGYP EDKHTVVDSN QTTDWVDYNI NFPVSGNYRI
260 270 280 290 300
EMLASGQTSH ATAILFVDNV QINEVAVDTG NQAVFLDFEL TDSTYISAGA
310 320 330 340 350
HTIRVQSGSQ INEFSWMWFG DALTFTPLDG GSTDGDADND GVLDSVDTCP
360 370 380 390 400
NTPAGAQVDA NGCEIIVDND TDNDGVDNSI DQCPNTPAGA QVDANGCEIV
410 420 430 440 450
AVVDADNDGV EDSLDMCPNT PAGAPVNGQG CADSQLDADN DGVSDDIDQC
460 470 480 490 500
PSTPAGSVVD GTGCIVVTPP ADSDNDGVVD TLDMCPNTAA GLTVDSQGCA
510 520 530 540 550
LSQLDSDNDG VTDDIDQCAN TPSGETANAT GCSSSQEGGG TDPDTPQPGL
560 570 580 590 600
LYGELAGAMN VSDTNPNWER TTDLLQTEDS VKGNTTEVYT GFIYDADGHI
610 620 630 640 650
SFYEHIDDSV RLYIDGVLVL SNDSWEASSQ TTDLNLTPGT HEIELRIGNA
660 670 680 690 700
DGGSGAVDGI GFGIDVDGGT NFVHPSTLSE SIFTSVGEET GNPDLEQEGD
710 720 730 740 750
IIVELESFVF TSTNGRVGSD SVEGFSPTAT GVNWVTNGDY GDYMVTFEEP
760 770 780 790 800
GTYGAYITIS AANDGSYGAR VDVDGWPVAW GYFGGTGSWD VSSENLLYGG
810 820 830 840 850
TFVVEQAGEK VVRVEAIGGS DWQWSGDRVR FTRLGDVTAI PSPIYNPDDH
860 870 880 890 900
FVAEIQGPQT DVTYLKKPVE IPANKKVLKS DVWYTYPQNR ELEGYDNFGA
910 920 930 940 950
TGAFWGHPPE HDFYDDTVIM DWAVDAVYAF QAEGYEYTAR GEFDWGYGWF
960 970 980 990 1000
TEYTTNPQPH YVRTLDDRNV RMTFMGYLSH DGYNNNWLSN HSPAFVPFMK
1010 1020 1030 1040 1050
SQVDQILKAN PDKLMFDTQT NSTRSTDMRD FGGDFSPYAM ENFRVWLSKK
1060 1070 1080 1090 1100
YSTGELAALG INDINSFDYG DFLRAQGVTH TSWSNAGDTL SGNIPLQEDY
1110 1120 1130 1140 1150
IYFNRDVWNQ KFAEVLDYIR QQQPDIEIGA STHLFESRGY VFNENLTFLS
1160 1170 1180 1190 1200
GELNLGARTT ISELPTNILV HLKGAQAVDK TLVYFPYPWE FDELRLQDAP
1210 1220 1230 1240 1250
RFGRGWVAQA YAYGGLFSIP ANVWVGGEVW TWSPGADNYR DIYLFVRAQA
1260 1270 1280 1290 1300
DLLDDYTSYS KVGLVHAMYS SMKAGFIDGG NQIQSSTKLL TEGNINFDLL
1310 1320 1330 1340 1350
VFGDEGYPVV PRPEDFDKFD HIFFDGDEQY LTAEQQALLD QQGDKVRHIG
1360 1370 1380 1390 1400
QRGTVSGIEI TVSISGTESN ETVSAVSRIH ETDAAAPYVV HLVNRPFAGG
1410 1420 1430 1440 1450
VTPTLNNVEV AIPQSYFPEV VTGATLHLPD GTSTSLTLST NADGDVVLPV
1460
NNLEVWGILE LAH
Length:1,463
Mass (Da):158,250
Last modified:February 6, 2007 - v1
Checksum:iADCB23F68C299BEC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB211981 Genomic DNA. Translation: BAF44076.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB211981 Genomic DNA. Translation: BAF44076.1 .

3D structure databases

ProteinModelPortali A1IGV8.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM6. Carbohydrate-Binding Module Family 6.
GH96. Glycoside Hydrolase Family 96.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16654.
BRENDAi 3.2.1.158. 8322.

Family and domain databases

Gene3Di 2.60.120.260. 3 hits.
3.90.182.10. 1 hit.
4.10.1080.10. 2 hits.
InterProi IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR011658. PA14.
IPR003367. Thrombospondin_3-like_rpt.
IPR028974. TSP_type-3_rpt.
[Graphical view ]
Pfami PF03422. CBM_6. 1 hit.
PF02412. TSP_3. 6 hits.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 2 hits.
SSF49785. SSF49785. 3 hits.
PROSITEi PS51175. CBM6. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Hyperproduction and application of alpha-agarase to enzymatic enhancement of antioxidant activity of porphyran."
    Hatada Y., Ohta Y., Horikoshi K.
    J. Agric. Food Chem. 54:9895-9900(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 685-696; 1181-1188 AND 1291-1298, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: JAMB-A33Imported.
  2. "Purification and characterization of a novel alpha-agarase from a Thalassomonas sp."
    Ohta Y., Hatada Y., Miyazaki M., Nogi Y., Ito S., Horikoshi K.
    Curr. Microbiol. 50:212-216(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1289-1298, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    Strain: JAMB-A331 Publication.

Entry informationi

Entry nameiAAGAR_THASX
AccessioniPrimary (citable) accession number: A1IGV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: February 6, 2007
Last modified: October 1, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3