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A1IGV8

- AAGAR_THASX

UniProt

A1IGV8 - AAGAR_THASX

Protein

Alpha-agarase

Gene

agaA33

Organism
Thalassotalea agarivorans (Thalassomonas agarivorans)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Alpha-agarase. Hydrolyzes agarose, agarohexaose, neoagarohexaose and porphyran. Hydrolysis of porphyran by this enzyme improves its antioxidant activity. Does not hydrolyze kappa-carrageenan, iota-carrageenen or lambda-carrageenan.2 Publications

    Catalytic activityi

    Endohydrolysis of 1,3-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product.2 Publications

    Cofactori

    Calcium.1 Publication

    pH dependencei

    Optimum pH is 8.5. Active between pH 4.5 and 9.5, stable between pH 6.5 and 10.5.2 Publications

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius. Stable up to 40 degrees Celsius.2 Publications

    GO - Molecular functioni

    1. alpha-agarase activity Source: UniProtKB-EC
    2. calcium ion binding Source: InterPro
    3. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. cell adhesion Source: InterPro
    2. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16654.
    BRENDAi3.2.1.158. 8322.

    Protein family/group databases

    CAZyiCBM6. Carbohydrate-Binding Module Family 6.
    GH96. Glycoside Hydrolase Family 96.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-agarase1 Publication (EC:3.2.1.158)
    Alternative name(s):
    AgaraseA331 Publication
    Gene namesi
    Name:agaA33Imported
    OrganismiThalassotalea agarivorans (Thalassomonas agarivorans)
    Taxonomic identifieri349064 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesColwelliaceaeThalassotalea

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Propeptidei28 – 6846571 PublicationSequence AnalysisPRO_0000397928Add
    BLAST
    Chaini685 – 1463779Alpha-agarase1 PublicationPRO_0000397929Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliA1IGV8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini701 – 832132CBM6PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 96 family.By similarity
    Contains 1 CBM6 (carbohydrate binding type-6) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.260. 3 hits.
    3.90.182.10. 1 hit.
    4.10.1080.10. 2 hits.
    InterProiIPR005084. CMB_fam6.
    IPR008979. Galactose-bd-like.
    IPR011658. PA14.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR028974. TSP_type-3_rpt.
    [Graphical view]
    PfamiPF03422. CBM_6. 1 hit.
    PF02412. TSP_3. 6 hits.
    [Graphical view]
    SUPFAMiSSF103647. SSF103647. 2 hits.
    SSF49785. SSF49785. 3 hits.
    PROSITEiPS51175. CBM6. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1IGV8-1 [UniParc]FASTAAdd to Basket

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    MITSSKKIVS AMLSTSLWIG VASAAYAETT NVEAEGYSTI GGTYQDGNPQ     50
    PINIYSVNGV QAINFVNRGD FAEYDVSVST AGEYSIEYLI GTSIASGSAV 100
    EISVLVDGNW QSAGSTNVPL GQWDNFQALA ANNNISLAQG TNRIKITGAG 150
    THDWQWNLDA FSLTLVTPEN PDNPDNPDNP DDGNTGQPGT PFTIEMEAFD 200
    ATGSDDPRAQ GMVIGERGYP EDKHTVVDSN QTTDWVDYNI NFPVSGNYRI 250
    EMLASGQTSH ATAILFVDNV QINEVAVDTG NQAVFLDFEL TDSTYISAGA 300
    HTIRVQSGSQ INEFSWMWFG DALTFTPLDG GSTDGDADND GVLDSVDTCP 350
    NTPAGAQVDA NGCEIIVDND TDNDGVDNSI DQCPNTPAGA QVDANGCEIV 400
    AVVDADNDGV EDSLDMCPNT PAGAPVNGQG CADSQLDADN DGVSDDIDQC 450
    PSTPAGSVVD GTGCIVVTPP ADSDNDGVVD TLDMCPNTAA GLTVDSQGCA 500
    LSQLDSDNDG VTDDIDQCAN TPSGETANAT GCSSSQEGGG TDPDTPQPGL 550
    LYGELAGAMN VSDTNPNWER TTDLLQTEDS VKGNTTEVYT GFIYDADGHI 600
    SFYEHIDDSV RLYIDGVLVL SNDSWEASSQ TTDLNLTPGT HEIELRIGNA 650
    DGGSGAVDGI GFGIDVDGGT NFVHPSTLSE SIFTSVGEET GNPDLEQEGD 700
    IIVELESFVF TSTNGRVGSD SVEGFSPTAT GVNWVTNGDY GDYMVTFEEP 750
    GTYGAYITIS AANDGSYGAR VDVDGWPVAW GYFGGTGSWD VSSENLLYGG 800
    TFVVEQAGEK VVRVEAIGGS DWQWSGDRVR FTRLGDVTAI PSPIYNPDDH 850
    FVAEIQGPQT DVTYLKKPVE IPANKKVLKS DVWYTYPQNR ELEGYDNFGA 900
    TGAFWGHPPE HDFYDDTVIM DWAVDAVYAF QAEGYEYTAR GEFDWGYGWF 950
    TEYTTNPQPH YVRTLDDRNV RMTFMGYLSH DGYNNNWLSN HSPAFVPFMK 1000
    SQVDQILKAN PDKLMFDTQT NSTRSTDMRD FGGDFSPYAM ENFRVWLSKK 1050
    YSTGELAALG INDINSFDYG DFLRAQGVTH TSWSNAGDTL SGNIPLQEDY 1100
    IYFNRDVWNQ KFAEVLDYIR QQQPDIEIGA STHLFESRGY VFNENLTFLS 1150
    GELNLGARTT ISELPTNILV HLKGAQAVDK TLVYFPYPWE FDELRLQDAP 1200
    RFGRGWVAQA YAYGGLFSIP ANVWVGGEVW TWSPGADNYR DIYLFVRAQA 1250
    DLLDDYTSYS KVGLVHAMYS SMKAGFIDGG NQIQSSTKLL TEGNINFDLL 1300
    VFGDEGYPVV PRPEDFDKFD HIFFDGDEQY LTAEQQALLD QQGDKVRHIG 1350
    QRGTVSGIEI TVSISGTESN ETVSAVSRIH ETDAAAPYVV HLVNRPFAGG 1400
    VTPTLNNVEV AIPQSYFPEV VTGATLHLPD GTSTSLTLST NADGDVVLPV 1450
    NNLEVWGILE LAH 1463
    Length:1,463
    Mass (Da):158,250
    Last modified:February 6, 2007 - v1
    Checksum:iADCB23F68C299BEC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB211981 Genomic DNA. Translation: BAF44076.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB211981 Genomic DNA. Translation: BAF44076.1 .

    3D structure databases

    ProteinModelPortali A1IGV8.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM6. Carbohydrate-Binding Module Family 6.
    GH96. Glycoside Hydrolase Family 96.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16654.
    BRENDAi 3.2.1.158. 8322.

    Family and domain databases

    Gene3Di 2.60.120.260. 3 hits.
    3.90.182.10. 1 hit.
    4.10.1080.10. 2 hits.
    InterProi IPR005084. CMB_fam6.
    IPR008979. Galactose-bd-like.
    IPR011658. PA14.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR028974. TSP_type-3_rpt.
    [Graphical view ]
    Pfami PF03422. CBM_6. 1 hit.
    PF02412. TSP_3. 6 hits.
    [Graphical view ]
    SUPFAMi SSF103647. SSF103647. 2 hits.
    SSF49785. SSF49785. 3 hits.
    PROSITEi PS51175. CBM6. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Hyperproduction and application of alpha-agarase to enzymatic enhancement of antioxidant activity of porphyran."
      Hatada Y., Ohta Y., Horikoshi K.
      J. Agric. Food Chem. 54:9895-9900(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 685-696; 1181-1188 AND 1291-1298, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: JAMB-A33Imported.
    2. "Purification and characterization of a novel alpha-agarase from a Thalassomonas sp."
      Ohta Y., Hatada Y., Miyazaki M., Nogi Y., Ito S., Horikoshi K.
      Curr. Microbiol. 50:212-216(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1289-1298, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: JAMB-A331 Publication.

    Entry informationi

    Entry nameiAAGAR_THASX
    AccessioniPrimary (citable) accession number: A1IGV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3