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A1DJ58

- BGALE_NEOFI

UniProt

A1DJ58 - BGALE_NEOFI

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Protein

Probable beta-galactosidase E

Gene

lacE

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921SubstrateBy similarity
Binding sitei136 – 1361SubstrateBy similarity
Binding sitei137 – 1371Substrate; via amide nitrogenBy similarity
Binding sitei138 – 1381SubstrateBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Active sitei196 – 1961Proton donorSequence Analysis
Binding sitei261 – 2611SubstrateBy similarity
Active sitei299 – 2991NucleophileSequence Analysis
Binding sitei365 – 3651SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase E (EC:3.2.1.23)
Alternative name(s):
Lactase E
Gene namesi
Name:lacE
ORF Names:NFIA_000910
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
ProteomesiUP000006702: Unassembled WGS sequence

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 1011992Probable beta-galactosidase EPRO_0000395244Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi267 ↔ 316By similarity
Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi588 – 5881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi704 – 7041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi745 – 7451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi759 – 7591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi772 – 7721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi778 – 7781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi913 – 9131N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi36630.CADNFIAP00000075.

Structurei

3D structure databases

ProteinModelPortaliA1DJ58.
SMRiA1DJ58. Positions 37-1011.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
OMAiGIDAYPM.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1DJ58-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFLLRRFIA LAAASSVVAA PSVSHLSLQD AANRRELLQD LVTWDQHSLF
60 70 80 90 100
VRGERLMIFS GEFHPFRLPV PGLWFDVFQK ITSLGFNAVS FYTDWGLMEG
110 120 130 140 150
NPGHVVTDGI WSLDEFFTAA SEAGIYLIAR PGPYINAETS AGGIPGWVLR
160 170 180 190 200
LKGIIRSNSE DYLRATDTYM ATLGKIIAKA QITNGGPVIL VQPENEYTTW
210 220 230 240 250
PNVSESEFPT TMNKEVMAYA EKQLRDAGVV VPTVVNDNKN LGYFAPGTGL
260 270 280 290 300
GETDLYGIDA YPMRYDCGNP YVWPTYRFPR DWQHTHRNHS PTTPFAIMEF
310 320 330 340 350
QGGSGDGWGG VTEDGCAILV NNEAVRVVYK NNYGFGVGVF NIYMTYGGTN
360 370 380 390 400
WGNLGYHGGY TSYDYGAAIT EDRQIWREKY SEEKLQANFL KVSPAYLTAT
410 420 430 440 450
PGNGVNGSYT GNKDIAVTPL FGNGTTTNFY LVRHADFTST GSVQYQLSVS
460 470 480 490 500
TSVGNVTIPQ LGGSLSLNGR DSKFHVTDYD VGEFNLIYSS AEIFTWAKGD
510 520 530 540 550
NKKRVLVLYG GAGELHEFAL PKHLPRPTVV DGSDVKMAKK GSAWVVQWEV
560 570 580 590 600
TAQRRVLRAG KLEIHLLWRN DAYQHWVLEL PAKQPIANYS SPSKETVLVK
610 620 630 640 650
GGYLLRSACI TNNKLHLTGD VNATTPLEVI SAPKRFDGIV FNGQSLKSTR
660 670 680 690 700
SKIGNLAATV RYQPPAISLP DLKRLDWKYL DSLPEISPDY SDEGWMSLTN
710 720 730 740 750
TYTNNTRKFT GPTCLYADDY GYHGGSLIYR GHFKANGDES WVFLNTSGGV
760 770 780 790 800
GFANSVWLNQ TFLGSWTGSG NNMTYPRNIS LPHELSPGKP YVFTVVIDHM
810 820 830 840 850
GQDEEAPGTD AIKFPRGILD YALSGHEVSD LKWKMTGNLG GEQYQDSTRG
860 870 880 890 900
PLNEGAMYAE RRGYHLPNPP TSSWKSSSPI NDGLTGAGIG FYATSFSLDL
910 920 930 940 950
PEGYDIPLSF LFNNSASDAR SGTSYRCQLF VNGYQFGKYV NDLGPQTNFP
960 970 980 990 1000
VPEGILNYNG VNYVAVSLWA LEPQGALVGG LELVASTPIL SAYRKPVPAP
1010
QPGWKPRRGA Y
Length:1,011
Mass (Da):111,421
Last modified:January 23, 2007 - v1
Checksum:i2E6518FE3F132FAC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS027697 Genomic DNA. Translation: EAW16747.1.
RefSeqiXP_001258644.1. XM_001258643.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00000084; CADNFIAP00000075; CADNFIAG00000084.
GeneIDi4585646.
KEGGinfi:NFIA_000910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS027697 Genomic DNA. Translation: EAW16747.1 .
RefSeqi XP_001258644.1. XM_001258643.1.

3D structure databases

ProteinModelPortali A1DJ58.
SMRi A1DJ58. Positions 37-1011.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 36630.CADNFIAP00000075.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADNFIAT00000084 ; CADNFIAP00000075 ; CADNFIAG00000084 .
GeneIDi 4585646.
KEGGi nfi:NFIA_000910.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000181922.
OMAi GIDAYPM.
OrthoDBi EOG7ZGXBD.

Family and domain databases

Gene3Di 2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SMARTi SM01029. BetaGal_dom2. 1 hit.
[Graphical view ]
SUPFAMi SSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Entry informationi

Entry nameiBGALE_NEOFI
AccessioniPrimary (citable) accession number: A1DJ58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3