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Protein

Probable beta-galactosidase E

Gene

lacE

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei136SubstrateBy similarity1
Binding sitei137Substrate; via amide nitrogenBy similarity1
Binding sitei138SubstrateBy similarity1
Binding sitei195SubstrateBy similarity1
Active sitei196Proton donorSequence analysis1
Binding sitei261SubstrateBy similarity1
Active sitei299NucleophileSequence analysis1
Binding sitei365SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase E (EC:3.2.1.23)
Alternative name(s):
Lactase E
Gene namesi
Name:lacE
ORF Names:NFIA_000910
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006702 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:NFIA_000910.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000039524420 – 1011Probable beta-galactosidase EAdd BLAST992

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi202N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi267 ↔ 316By similarity
Glycosylationi406N-linked (GlcNAc...)Sequence analysis1
Glycosylationi423N-linked (GlcNAc...)Sequence analysis1
Glycosylationi455N-linked (GlcNAc...)Sequence analysis1
Glycosylationi588N-linked (GlcNAc...)Sequence analysis1
Glycosylationi622N-linked (GlcNAc...)Sequence analysis1
Glycosylationi704N-linked (GlcNAc...)Sequence analysis1
Glycosylationi745N-linked (GlcNAc...)Sequence analysis1
Glycosylationi759N-linked (GlcNAc...)Sequence analysis1
Glycosylationi772N-linked (GlcNAc...)Sequence analysis1
Glycosylationi778N-linked (GlcNAc...)Sequence analysis1
Glycosylationi913N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliA1DJ58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922.
OMAiGIDAYPM.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1DJ58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLLRRFIA LAAASSVVAA PSVSHLSLQD AANRRELLQD LVTWDQHSLF
60 70 80 90 100
VRGERLMIFS GEFHPFRLPV PGLWFDVFQK ITSLGFNAVS FYTDWGLMEG
110 120 130 140 150
NPGHVVTDGI WSLDEFFTAA SEAGIYLIAR PGPYINAETS AGGIPGWVLR
160 170 180 190 200
LKGIIRSNSE DYLRATDTYM ATLGKIIAKA QITNGGPVIL VQPENEYTTW
210 220 230 240 250
PNVSESEFPT TMNKEVMAYA EKQLRDAGVV VPTVVNDNKN LGYFAPGTGL
260 270 280 290 300
GETDLYGIDA YPMRYDCGNP YVWPTYRFPR DWQHTHRNHS PTTPFAIMEF
310 320 330 340 350
QGGSGDGWGG VTEDGCAILV NNEAVRVVYK NNYGFGVGVF NIYMTYGGTN
360 370 380 390 400
WGNLGYHGGY TSYDYGAAIT EDRQIWREKY SEEKLQANFL KVSPAYLTAT
410 420 430 440 450
PGNGVNGSYT GNKDIAVTPL FGNGTTTNFY LVRHADFTST GSVQYQLSVS
460 470 480 490 500
TSVGNVTIPQ LGGSLSLNGR DSKFHVTDYD VGEFNLIYSS AEIFTWAKGD
510 520 530 540 550
NKKRVLVLYG GAGELHEFAL PKHLPRPTVV DGSDVKMAKK GSAWVVQWEV
560 570 580 590 600
TAQRRVLRAG KLEIHLLWRN DAYQHWVLEL PAKQPIANYS SPSKETVLVK
610 620 630 640 650
GGYLLRSACI TNNKLHLTGD VNATTPLEVI SAPKRFDGIV FNGQSLKSTR
660 670 680 690 700
SKIGNLAATV RYQPPAISLP DLKRLDWKYL DSLPEISPDY SDEGWMSLTN
710 720 730 740 750
TYTNNTRKFT GPTCLYADDY GYHGGSLIYR GHFKANGDES WVFLNTSGGV
760 770 780 790 800
GFANSVWLNQ TFLGSWTGSG NNMTYPRNIS LPHELSPGKP YVFTVVIDHM
810 820 830 840 850
GQDEEAPGTD AIKFPRGILD YALSGHEVSD LKWKMTGNLG GEQYQDSTRG
860 870 880 890 900
PLNEGAMYAE RRGYHLPNPP TSSWKSSSPI NDGLTGAGIG FYATSFSLDL
910 920 930 940 950
PEGYDIPLSF LFNNSASDAR SGTSYRCQLF VNGYQFGKYV NDLGPQTNFP
960 970 980 990 1000
VPEGILNYNG VNYVAVSLWA LEPQGALVGG LELVASTPIL SAYRKPVPAP
1010
QPGWKPRRGA Y
Length:1,011
Mass (Da):111,421
Last modified:January 23, 2007 - v1
Checksum:i2E6518FE3F132FAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027697 Genomic DNA. Translation: EAW16747.1.
RefSeqiXP_001258644.1. XM_001258643.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00000084; CADNFIAP00000075; CADNFIAG00000084.
GeneIDi4585646.
KEGGinfi:NFIA_000910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027697 Genomic DNA. Translation: EAW16747.1.
RefSeqiXP_001258644.1. XM_001258643.1.

3D structure databases

ProteinModelPortaliA1DJ58.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADNFIAT00000084; CADNFIAP00000075; CADNFIAG00000084.
GeneIDi4585646.
KEGGinfi:NFIA_000910.

Organism-specific databases

EuPathDBiFungiDB:NFIA_000910.

Phylogenomic databases

HOGENOMiHOG000181922.
OMAiGIDAYPM.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGALE_NEOFI
AccessioniPrimary (citable) accession number: A1DJ58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.