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A1DJ58

- BGALE_NEOFI

UniProt

A1DJ58 - BGALE_NEOFI

Protein

Probable beta-galactosidase E

Gene

lacE

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921SubstrateBy similarity
    Binding sitei136 – 1361SubstrateBy similarity
    Binding sitei137 – 1371Substrate; via amide nitrogenBy similarity
    Binding sitei138 – 1381SubstrateBy similarity
    Binding sitei195 – 1951SubstrateBy similarity
    Active sitei196 – 1961Proton donorSequence Analysis
    Binding sitei261 – 2611SubstrateBy similarity
    Active sitei299 – 2991NucleophileSequence Analysis
    Binding sitei365 – 3651SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable beta-galactosidase E (EC:3.2.1.23)
    Alternative name(s):
    Lactase E
    Gene namesi
    Name:lacE
    ORF Names:NFIA_000910
    OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
    Taxonomic identifieri331117 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
    ProteomesiUP000006702: Unassembled WGS sequence

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 1011992Probable beta-galactosidase EPRO_0000395244Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi267 ↔ 316By similarity
    Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi588 – 5881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi704 – 7041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi745 – 7451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi759 – 7591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi772 – 7721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi778 – 7781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi913 – 9131N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi36630.CADNFIAP00000075.

    Structurei

    3D structure databases

    ProteinModelPortaliA1DJ58.
    SMRiA1DJ58. Positions 37-1011.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000181922.
    OMAiGIDAYPM.
    OrthoDBiEOG7ZGXBD.

    Family and domain databases

    Gene3Di2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SMARTiSM01029. BetaGal_dom2. 1 hit.
    [Graphical view]
    SUPFAMiSSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1DJ58-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFLLRRFIA LAAASSVVAA PSVSHLSLQD AANRRELLQD LVTWDQHSLF     50
    VRGERLMIFS GEFHPFRLPV PGLWFDVFQK ITSLGFNAVS FYTDWGLMEG 100
    NPGHVVTDGI WSLDEFFTAA SEAGIYLIAR PGPYINAETS AGGIPGWVLR 150
    LKGIIRSNSE DYLRATDTYM ATLGKIIAKA QITNGGPVIL VQPENEYTTW 200
    PNVSESEFPT TMNKEVMAYA EKQLRDAGVV VPTVVNDNKN LGYFAPGTGL 250
    GETDLYGIDA YPMRYDCGNP YVWPTYRFPR DWQHTHRNHS PTTPFAIMEF 300
    QGGSGDGWGG VTEDGCAILV NNEAVRVVYK NNYGFGVGVF NIYMTYGGTN 350
    WGNLGYHGGY TSYDYGAAIT EDRQIWREKY SEEKLQANFL KVSPAYLTAT 400
    PGNGVNGSYT GNKDIAVTPL FGNGTTTNFY LVRHADFTST GSVQYQLSVS 450
    TSVGNVTIPQ LGGSLSLNGR DSKFHVTDYD VGEFNLIYSS AEIFTWAKGD 500
    NKKRVLVLYG GAGELHEFAL PKHLPRPTVV DGSDVKMAKK GSAWVVQWEV 550
    TAQRRVLRAG KLEIHLLWRN DAYQHWVLEL PAKQPIANYS SPSKETVLVK 600
    GGYLLRSACI TNNKLHLTGD VNATTPLEVI SAPKRFDGIV FNGQSLKSTR 650
    SKIGNLAATV RYQPPAISLP DLKRLDWKYL DSLPEISPDY SDEGWMSLTN 700
    TYTNNTRKFT GPTCLYADDY GYHGGSLIYR GHFKANGDES WVFLNTSGGV 750
    GFANSVWLNQ TFLGSWTGSG NNMTYPRNIS LPHELSPGKP YVFTVVIDHM 800
    GQDEEAPGTD AIKFPRGILD YALSGHEVSD LKWKMTGNLG GEQYQDSTRG 850
    PLNEGAMYAE RRGYHLPNPP TSSWKSSSPI NDGLTGAGIG FYATSFSLDL 900
    PEGYDIPLSF LFNNSASDAR SGTSYRCQLF VNGYQFGKYV NDLGPQTNFP 950
    VPEGILNYNG VNYVAVSLWA LEPQGALVGG LELVASTPIL SAYRKPVPAP 1000
    QPGWKPRRGA Y 1011
    Length:1,011
    Mass (Da):111,421
    Last modified:January 23, 2007 - v1
    Checksum:i2E6518FE3F132FAC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027697 Genomic DNA. Translation: EAW16747.1.
    RefSeqiXP_001258644.1. XM_001258643.1.

    Genome annotation databases

    EnsemblFungiiCADNFIAT00000084; CADNFIAP00000075; CADNFIAG00000084.
    GeneIDi4585646.
    KEGGinfi:NFIA_000910.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027697 Genomic DNA. Translation: EAW16747.1 .
    RefSeqi XP_001258644.1. XM_001258643.1.

    3D structure databases

    ProteinModelPortali A1DJ58.
    SMRi A1DJ58. Positions 37-1011.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 36630.CADNFIAP00000075.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADNFIAT00000084 ; CADNFIAP00000075 ; CADNFIAG00000084 .
    GeneIDi 4585646.
    KEGGi nfi:NFIA_000910.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000181922.
    OMAi GIDAYPM.
    OrthoDBi EOG7ZGXBD.

    Family and domain databases

    Gene3Di 2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SMARTi SM01029. BetaGal_dom2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

    Entry informationi

    Entry nameiBGALE_NEOFI
    AccessioniPrimary (citable) accession number: A1DJ58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3