ID   NCB5R_NEOFI             Reviewed;         309 AA.
AC   A1DHW1;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 2.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=NADH-cytochrome b5 reductase 1;
DE            EC=1.6.2.2;
DE   AltName: Full=Microsomal cytochrome b reductase;
GN   Name=cbr1; ORFNames=NFIA_089250;
OS   Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL
OS   181) (Aspergillus fischerianus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae; Neosartorya.
OX   NCBI_TaxID=331117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P.,
RA   Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A.,
RA   Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A.,
RA   Galens K., Fraser-Liggett C.M., Haas B.J., Inman J.M., Kent R.,
RA   Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M.,
RA   Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R.,
RA   Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R.,
RA   Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Electron donor reductase for cytochrome b5. The
CC       cytochrome b5/NADH cytochrome b5 reductase electron transfer
CC       system supports the catalytic activity of several sterol
CC       biosynthetic enzymes (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD(+) + H(+) +
CC       2 ferrocytochrome b5.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein (By similarity). Mitochondrion outer membrane;
CC       Single-pass membrane protein (By similarity).
CC   -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide
CC       cytochrome reductase family.
CC   -!- SIMILARITY: Contains 1 FAD-binding FR-type domain.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAW18968.1; Type=Erroneous gene model prediction;
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DR   EMBL; DS027696; EAW18968.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_001260865.1; -.
DR   GeneID; 4587423; -.
DR   KEGG; nfi:NFIA_089250; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004128; F:cytochrome-b5 reductase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017927; Fd_Rdtase_FAD-bd.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR001834; NADH-Cyt_B5_reductase.
DR   InterPro; IPR008333; OxRdtase_FAD-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD_bd.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00371; FPNCR.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Endoplasmic reticulum; FAD; Flavoprotein; Membrane;
KW   Mitochondrion; Mitochondrion outer membrane; NAD; Oxidoreductase;
KW   Transmembrane.
FT   CHAIN         1    309       NADH-cytochrome b5 reductase 1.
FT                                /FTId=PRO_0000330159.
FT   TRANSMEM     30     50       Potential.
FT   DOMAIN       60    165       FAD-binding FR-type.
FT   NP_BIND     145    160       FAD (By similarity).
FT   NP_BIND     171    208       FAD (By similarity).
SQ   SEQUENCE   309 AA;  33756 MW;  A68FB1319E938C93 CRC64;
     MSALSSENIN GVYIPSALLI FGTFIVKKEF VPYAVALTAI LAGLKLFTGG SKPRKVLNPT
     EFQEFVLKEK TDISHNVCIY RFALPRPADI LGLPIGQHIS LAATIEGQPK EVVRSYTPIS
     SDNEAGYFDL LVKAYPQGNI SKYLTTLKIG DTMKVRGPKG AMVYTPNMCR HIGMIAGGTG
     ITPMLQIIKA VIRNRPRNGG NDTTKLDLIF ANVNPDDILL KEELDKLAAE DPDFNIYYVL
     NNPPQGWTGG VGFVTPEMIK EHLPAPASDV KILLCGPPPM ISAMKKATES LGYTKARPVS
     KLEDQVFCF
//