A1DH89 (CREA_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable DNA-binding protein creA

Alternative name(s):
Carbon catabolite repressor A

Gene names

Name:	creA
ORF Names:	NFIA_087020

Organism

Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]

Taxonomic identifier

331117 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Neosartorya › Neosartorya fischeri group › Neosartorya fischeri

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transcription regulator component of the regulatory network controlling carbon source utilization through ubiquitination and deubiquitination involving creA, creB, creC, creD and acrB. Represses the transcription of the alcR, alcA and aldA genes by binding to a GC-rich region in their promoter. Plays also a role in response to carbon starvation and the control of extracellular proteases activity By similarity.
Subunit structure	Interacts with creB By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Ubiquitinated. Deubiquitinated by creB, probably to control its activity or amount By similarity.
Sequence similarities	Belongs to the creA/MIG C2H2-type zinc-finger protein family. Contains 2 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Probable DNA-binding protein creA		PRO_0000395725
Regions
	Zinc finger	66 – 88	23	C2H2-type 1
	Zinc finger	94 – 118	25	C2H2-type 2
	Compositional bias	133 – 141	9	Poly-Ala
	Compositional bias	280 – 384	105	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	A1DH89 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 7063E4850EE2288B	FASTA	416	44,999
	10 20 30 40 50 60 MGFSDLLNPQ NPESTPSTPA NKSSAPSTPS TGQSNSNMTS VSLLPPLMKG ARPANEEPRQ 70 80 90 100 110 120 DLPRPYKCPL CDRAFHRLEH QTRHIRTHTG EKPHACQFPG CTKRFSRSDE LTRHSRIHNN 130 140 150 160 170 180 PNSRRNNKAQ HLAAAAAAAA ANQDNALASN AASMMPPPSK PITRSAPVSQ VGSPDISPPH 190 200 210 220 230 240 SFSNYAGHMR SNLGPYARNS DRASSGMDIN LLATAASQVE RDEHYGFHNG PRGHHIFGSR 250 260 270 280 290 300 HHNSNRLPSL SAYAISQNMS RSHSHDEDDM YSHRVKRSRP NSPNSTAPSS PTFSHDSLSP 310 320 330 340 350 360 TPDHTPLATP AHSPRLRPLG TSELQLPSIR HLSLHHTPAL APMEPQPEGP NYYSPTQPHV 370 380 390 400 410 GPSISDIMSK PDGTQRKLPV PQVPKVAVQD LLSPGFTSVS SSASNSVAGG DLADRF

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027696 Genomic DNA. Translation: EAW18746.1.
RefSeq	XP_001260643.1. XM_001260642.1.
3D structure databases
ProteinModelPortal	A1DH89.
ModBase	Search...
Protein-protein interaction databases
STRING	36630.CADNFIAP00007056.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADNFIAT00007223; CADNFIAP00007056; CADNFIAG00007223.
GeneID	4587201.
KEGG	nfi:NFIA_087020.
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000164217.
KO	K09467.
OrthoDB	EOG41G6D6.
Family and domain databases
Gene3D	3.30.160.60. 2 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 2 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 2 hits. PS50157. ZINC_FINGER_C2H2_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CREA_NEOFI

Accession

Primary (citable) accession number: A1DH89

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 13, 2010
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program