Reviewed, UniProtKB/Swiss-Prot A1DH10 (ATG15_NEOFI)

Names and origin

Protein names

Recommended name:
    Putative lipase atg15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	atg15
ORF Names:	NFIA_086220

Organism

Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]

Taxonomic identifier

331117 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Neosartorya

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 634	634	Putative lipase atg15		PRO_0000317965
Regions
	Topological domain	1 – 4	4	Cytoplasmic By similarity
	Transmembrane	5 – 25	21	Signal-anchor for type II membrane protein
	Topological domain	26 – 634	609	Lumenal By similarity
Sites
	Active site	305	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	150	1	N-linked (GlcNAc...) Potential
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Glycosylation	265	1	N-linked (GlcNAc...) Potential
	Glycosylation	289	1	N-linked (GlcNAc...) Potential
	Glycosylation	451	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A1DH10-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 349855D3C98461E2	FASTA	634	68,394
	10 20 30 40 50 60 MSISGLLLSV ALLPSVVSAH DHVYFDPPSS GSPFLGPQIP LTGPPALTGT HEFTLRHIYQ 70 80 90 100 110 120 RGTRDQPDLH RRLDIKPHTR LWAVSDDGLE KELVTFDTPL VASSSPLTIQ RLADRRLSVI 130 140 150 160 170 180 EGYLAAARSS GEAVALSPSE WVMDTLAGPN VTDKESVLTF AKMTANDYIE EPGTEDWHYI 190 200 210 220 230 240 HGRFNYSSSF GWQSDGLRGH IYADKTNSTI VISLKGTSPA LFDGAGTTTN DKINDNLFFS 250 260 270 280 290 300 CCCGQGGSYL WRQVCDCQQS AFTANLTCIV EAMNDENRYY RAAIDLYSNV TDMYPDANVW 310 320 330 340 350 360 MTGHSLGGAM SSLLGLTFGL PVVTFEAVPE ALPAARLGLP SPPGHDPRLP QSRQYTGAYH 370 380 390 400 410 420 FGHTADPVYM GTCNGVGSIC TWGGYAMESA CHTGQMCVYD TVEDKGWRVA LSTHRIRAVI 430 440 450 460 470 480 SDVLEVYEDL PPCAPEEECY DCELWKFFKS NGSESTTTST TTTTTAPTTT RTSTCKTPGW 490 500 510 520 530 540 WGCLDESTTT TTITSTTTTT TTSTSTCKTP GWFGCKDPTT TTEATPAPSV TTTIPTPTTY 550 560 570 580 590 600 PTSSTSTCKD PGWFGCRDPS STTASITSSP STTSTCDDPG FFWGCYDEST TATHPITPGP 610 620 630 SAPYSTPSPT NKHTCTSSIF FGLICVGSTG TELR

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed: 18404212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS027696 Genomic DNA. Translation: EAW18667.1.
RefSeq	XP_001260564.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4587122.
KEGG	nfi:NFIA_086220.
Family and domain databases
InterPro	IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_NEOFI

Accession

Primary (citable) accession number: A1DH10

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)