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A1DFW7 (DOHH_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deoxyhypusine hydroxylase
Short name=DOHH
EC=1.14.99.29
Alternative name(s):
Deoxyhypusine dioxygenase
Deoxyhypusine monooxygenase
Gene names
Name:lia1
ORF Names:NFIA_082210
OrganismNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]
Taxonomic identifier331117 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Neosartorya ›

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor By similarity. HAMAP-Rule MF_03101

Catalytic activity

Protein N(6)-(4-aminobutyl)-L-lysine + AH2 + O2 = protein N(6)-((R)-4-amino-2-hydroxybutyl)-L-lysine + A + H2O. HAMAP-Rule MF_03101

Cofactor

Binds 2 Fe2+ ions per subunit By similarity.

Pathway

Protein modification; eIF5A hypusination. HAMAP-Rule MF_03101

Subcellular location

Cytoplasm. Nucleus By similarity.

Sequence similarities

Belongs to the deoxyhypusine hydroxylase family.

Contains 5 HEAT-like PBS-type repeats.

Ontologies

Keywords
   Biological processHypusine biosynthesis
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
   LigandIron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmicrotubule cytoskeleton organization

Inferred from electronic annotation. Source: EnsemblFungi

peptidyl-lysine modification to hypusine

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondeoxyhypusine monooxygenase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Deoxyhypusine hydroxylase HAMAP-Rule MF_03101
PRO_0000283669

Regions

Repeat71 – 9727HEAT-like PBS-type 1 HAMAP-Rule MF_03101
Repeat104 – 13027HEAT-like PBS-type 2 HAMAP-Rule MF_03101
Repeat200 – 23334HEAT-like PBS-type 3 HAMAP-Rule MF_03101
Repeat238 – 26427HEAT-like PBS-type 4 HAMAP-Rule MF_03101
Repeat271 – 29828HEAT-like PBS-type 5 HAMAP-Rule MF_03101

Sites

Metal binding731Iron 1 By similarity
Metal binding741Iron 1 By similarity
Metal binding1061Iron 1 By similarity
Metal binding1071Iron 1 By similarity
Metal binding2401Iron 2 By similarity
Metal binding2411Iron 2 By similarity
Metal binding2731Iron 2 By similarity
Metal binding2741Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1DFW7 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: C0385EE7460F5FCE

FASTA33536,482
        10         20         30         40         50         60 
MSPSATDTTE GVDPTILTLR KVLTSESEPL ARRFRALFSL KHLACLQPPT EKTLPAIQAI 

        70         80         90        100        110        120 
AAAFTSPSAL LKHELAYCLG QTRNPESVPF LQQVAKDTEQ DTMCRHEAAE ALGALGYEDS 

       130        140        150        160        170        180 
LEILKALRDN QNESDVIRET CDIAVDRILW ENSEARKAEK LKTSDFTSID PAPPLPMTAS 

       190        200        210        220        230        240 
EPSIPEIEKT LLDTSLPLFL RYRAMFALRD LASPPDLPTA TRAVEALAKG LKDPSALFRH 

       250        260        270        280        290        300 
EIAFVFGQLC HPASIPSLTE ALSNQSEAGM VRHEAAEALG SLGDCEGVEE TLRKFLNDPE 

       310        320        330 
QVVRDSVIVA LDMAEYEKNG EVEYALVPDA GVAAA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		DS027696 Genomic DNA. Translation: EAW18274.1.
RefSeqXP_001260171.1. XM_001260170.1.

3D structure databases

ProteinModelPortalA1DFW7.
ModBaseSearch...

Protein-protein interaction databases

STRING36630.CADNFIAP00007001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADNFIAT00007168; CADNFIAP00007001; CADNFIAG00007168.
GeneID4586728.
KEGGnfi:NFIA_082210.

Phylogenomic databases

eggNOGCOG1413.
HOGENOMHOG000248665.
KOK06072.
OrthoDBEOG4MSH78.

Enzyme and pathway databases

UniPathwayUPA00354.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
HAMAPMF_03101. Deoxyhypusine_hydroxylase.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMSSF48371. ARM-type_fold. 1 hit.
PROSITEPS50077. HEAT_REPEAT. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDOHH_NEOFI
AccessionPrimary (citable) accession number: A1DFW7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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