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A1DE13 (DCL1_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dicer-like protein 1

Including the following 2 domains:

  1. Endoribonuclease dcl1
    EC=3.1.26.-
  2. ATP-dependent helicase dcl1
    EC=3.6.4.-
Gene names
Name:dcl1
ORF Names:NFIA_075500
OrganismNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]
Taxonomic identifier331117 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya

Protein attributes

Sequence length1538 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.

Cofactor

Magnesium or manganese By similarity.

Sequence similarities

Belongs to the helicase family. Dicer subfamily.

Contains 1 Dicer dsRNA-binding fold domain.

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 2 RNase III domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15381538Dicer-like protein 1
PRO_0000306782

Regions

Domain134 – 315182Helicase ATP-binding
Domain460 – 619160Helicase C-terminal
Domain652 – 74291Dicer dsRNA-binding fold
Domain1044 – 1203160RNase III 1
Domain1254 – 1406153RNase III 2
Domain1440 – 150869DRBM
Nucleotide binding147 – 1548ATP By similarity
Motif260 – 2634DEAH box

Sites

Metal binding12951Magnesium or manganese By similarity
Metal binding13921Magnesium or manganese By similarity
Metal binding13951Magnesium or manganese By similarity
Site13881Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A1DE13 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 22BFCACC1932BB46

FASTA1,538174,902
        10         20         30         40         50         60 
MTEQISLVDV SSSVSLKGED SSNVALLRDL FINDVAASDP AESSADVHKD EHSSDNSDND 

        70         80         90        100        110        120 
NEAVPKPNDF SQRRRIQNAQ FEALLSKRTD ADSNEAIDRA PIALSDDELS IAHLVEKQDL 

       130        140        150        160        170        180 
GNGMLDPREY QIELFERAKT QNTIAVLDTG SGKTLIAVLL LRHTILNELD NRANGKPHRV 

       190        200        210        220        230        240 
SFFLVDSVTL AYQQAAVLRN NIDQNVAHFF GAMGTDLWDK RTWDEHLQRN MVIVCTAEIL 

       250        260        270        280        290        300 
NQCLLNSYVK MDQINLLIFD EAHHAKKDHP YARIIRDSYF KAQPSQRPRV FGMTASPIDT 

       310        320        330        340        350        360 
KGDITEAATR LETLLDSRIA TTSKITLLRE VVSRPIEKVW AYNRLESPFA TELYKLMDTR 

       370        380        390        400        410        420 
YGNIKVLEGV YRFAWHASSE LGKWCSDRAW WHALADDVLP KLEGNISKLV ESNTLNAEHG 

       430        440        450        460        470        480 
AVFKDIIRIR EASETVKNYS FADPELPGEL SPKVQLLRME LSKHFSDTTG TKCIVFTQKR 

       490        500        510        520        530        540 
YTAKILNELF TVLNIPHLRP GVLIGVRPGD IGGMNVTFRQ QFLALVKFRT GEINCLFATS 

       550        560        570        580        590        600 
VAEEGLDIPD CNLVVRFDLY RTLIQYVQSR GRARHCTSTY AIMVEKDNAE HEGRLKEIRE 

       610        620        630        640        650        660 
AEKIMQRFCE TLPEDRILHG NDHDLDSLLQ EEEGRRTFTV KSTGAKLTYH SAIAILARYA 

       670        680        690        700        710        720 
SSLQYEKETV PQVTYVVGHV SNAYVCEVIL PEKSPIRGLT GSPAIRKAVA KQSAAFDTCL 

       730        740        750        760        770        780 
LLRKHRLLDD YFNSIYHRRL PAMRNAKLAI TCKRTNEYDM LLKPSIWAKQ RTTPIDKLYG 

       790        800        810        820        830        840 
IHISLLPSKP LSRDHRPILL LTREKLPEFP AFSIYLDEDV ETKVLSYPLK HGLQISVDEL 

       850        860        870        880        890        900 
QSLTTFTLRI FRDIFHKVYE HEVQKMPYWL APAKALDGPG SGTNPRDWTD WDTVSFVHNN 

       910        920        930        940        950        960 
DEIPFTRDLN PDSLVNRFIF DNWDGRFRYF TVAVADTLQP SDPPPPSVPR RRHMNNIMNY 

       970        980        990       1000       1010       1020 
TLSLSKNSRA RFFSGCDWNQ PVLQAELVRL RRNLLDKMTT QEKEMQTECF ICAEPLRISA 

      1030       1040       1050       1060       1070       1080 
IPTSIVSTCL AFPAIISRLD SYLIALEACD ELELVIRPDF ALEAFTKDSD NTEEHRGQQI 

      1090       1100       1110       1120       1130       1140 
HFQRGMGKNY ERLEFLGDCF LKMATSIALY TQNPDDDEFD YHVNRMCLIC NKNLFNTAIK 

      1150       1160       1170       1180       1190       1200 
KQIYRYIRSR GFSRHIWYPD GLTLLHGKDH SKKLLSEGKH ALGEKTIADV CEALIGASLL 

      1210       1220       1230       1240       1250       1260 
SGGPENRFDM ATKAVTALVD SPSHRVSCWK EYITLYTMPK YQTEKPRGSE DDLARHVEEE 

      1270       1280       1290       1300       1310       1320 
LGYHFTYPRL LASAITHPSL PSTWGYRVPC YQRLEFLGDS LLDMVCVEDL FRRFPDRDPQ 

      1330       1340       1350       1360       1370       1380 
WLTEHKMAMV SNKFLGALSV KLGFHRRIMA FSNPLQAQIT HYVEEIESAQ AESRGAVDYW 

      1390       1400       1410       1420       1430       1440 
VVAKDPPKCL PDMVEAYLGA IFVDSKFDFQ VIEVFFERQI KPFFEDMSIY DTFANKHPTT 

      1450       1460       1470       1480       1490       1500 
FLHNKLTNEY GCTNYCLKAG ELPTIDGAPA GVLAAVIVHG NVISEARSSS SRYAKVKASE 

      1510       1520       1530 
KALAVLDGLL PFEFCQKYHC GCKETQNSSS AVEIGTAI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS027696 Genomic DNA. Translation: EAW17620.1.
RefSeqXP_001259517.1. XM_001259516.1.

3D structure databases

ProteinModelPortalA1DE13.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING36630.CADNFIAP00007382.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADNFIAT00007549; CADNFIAP00007382; CADNFIAG00007549.
GeneID4586299.
KEGGnfi:NFIA_075500.

Phylogenomic databases

eggNOGCOG1111.
HOGENOMHOG000048448.
KOK11592.
OMAGSWCADQ.
OrthoDBEOG76QFRP.

Family and domain databases

Gene3D1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProIPR005034. Dicer_dimerisation_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDCL1_NEOFI
AccessionPrimary (citable) accession number: A1DE13
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families