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A1DE13

- DCL1_NEOFI

UniProt

A1DE13 - DCL1_NEOFI

Protein

Dicer-like protein 1

Gene

dcl1

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.By similarity

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1295 – 12951Magnesium or manganeseBy similarity
    Sitei1388 – 13881Important for activityBy similarity
    Metal bindingi1392 – 13921Magnesium or manganeseBy similarity
    Metal bindingi1395 – 13951Magnesium or manganeseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi147 – 1548ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. ribonuclease III activity Source: InterPro
    5. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. regulation of defense response to virus Source: UniProtKB-KW
    3. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Antiviral protein, Helicase, Hydrolase

    Keywords - Biological processi

    Antiviral defense

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dicer-like protein 1
    Including the following 2 domains:
    Endoribonuclease dcl1 (EC:3.1.26.-)
    ATP-dependent helicase dcl1 (EC:3.6.4.-)
    Gene namesi
    Name:dcl1
    ORF Names:NFIA_075500
    OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
    Taxonomic identifieri331117 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
    ProteomesiUP000006702: Unassembled WGS sequence

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15381538Dicer-like protein 1PRO_0000306782Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi36630.CADNFIAP00007382.

    Structurei

    3D structure databases

    ProteinModelPortaliA1DE13.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini134 – 315182Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini460 – 619160Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini652 – 74291Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
    BLAST
    Domaini1044 – 1203160RNase III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1254 – 1406153RNase III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1440 – 150869DRBMAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi260 – 2634DEAH box

    Sequence similaritiesi

    Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
    Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 2 RNase III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1111.
    HOGENOMiHOG000048448.
    KOiK11592.
    OMAiNERPRIL.
    OrthoDBiEOG76QFRP.

    Family and domain databases

    Gene3Di1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProiIPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEiPS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1DE13-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEQISLVDV SSSVSLKGED SSNVALLRDL FINDVAASDP AESSADVHKD     50
    EHSSDNSDND NEAVPKPNDF SQRRRIQNAQ FEALLSKRTD ADSNEAIDRA 100
    PIALSDDELS IAHLVEKQDL GNGMLDPREY QIELFERAKT QNTIAVLDTG 150
    SGKTLIAVLL LRHTILNELD NRANGKPHRV SFFLVDSVTL AYQQAAVLRN 200
    NIDQNVAHFF GAMGTDLWDK RTWDEHLQRN MVIVCTAEIL NQCLLNSYVK 250
    MDQINLLIFD EAHHAKKDHP YARIIRDSYF KAQPSQRPRV FGMTASPIDT 300
    KGDITEAATR LETLLDSRIA TTSKITLLRE VVSRPIEKVW AYNRLESPFA 350
    TELYKLMDTR YGNIKVLEGV YRFAWHASSE LGKWCSDRAW WHALADDVLP 400
    KLEGNISKLV ESNTLNAEHG AVFKDIIRIR EASETVKNYS FADPELPGEL 450
    SPKVQLLRME LSKHFSDTTG TKCIVFTQKR YTAKILNELF TVLNIPHLRP 500
    GVLIGVRPGD IGGMNVTFRQ QFLALVKFRT GEINCLFATS VAEEGLDIPD 550
    CNLVVRFDLY RTLIQYVQSR GRARHCTSTY AIMVEKDNAE HEGRLKEIRE 600
    AEKIMQRFCE TLPEDRILHG NDHDLDSLLQ EEEGRRTFTV KSTGAKLTYH 650
    SAIAILARYA SSLQYEKETV PQVTYVVGHV SNAYVCEVIL PEKSPIRGLT 700
    GSPAIRKAVA KQSAAFDTCL LLRKHRLLDD YFNSIYHRRL PAMRNAKLAI 750
    TCKRTNEYDM LLKPSIWAKQ RTTPIDKLYG IHISLLPSKP LSRDHRPILL 800
    LTREKLPEFP AFSIYLDEDV ETKVLSYPLK HGLQISVDEL QSLTTFTLRI 850
    FRDIFHKVYE HEVQKMPYWL APAKALDGPG SGTNPRDWTD WDTVSFVHNN 900
    DEIPFTRDLN PDSLVNRFIF DNWDGRFRYF TVAVADTLQP SDPPPPSVPR 950
    RRHMNNIMNY TLSLSKNSRA RFFSGCDWNQ PVLQAELVRL RRNLLDKMTT 1000
    QEKEMQTECF ICAEPLRISA IPTSIVSTCL AFPAIISRLD SYLIALEACD 1050
    ELELVIRPDF ALEAFTKDSD NTEEHRGQQI HFQRGMGKNY ERLEFLGDCF 1100
    LKMATSIALY TQNPDDDEFD YHVNRMCLIC NKNLFNTAIK KQIYRYIRSR 1150
    GFSRHIWYPD GLTLLHGKDH SKKLLSEGKH ALGEKTIADV CEALIGASLL 1200
    SGGPENRFDM ATKAVTALVD SPSHRVSCWK EYITLYTMPK YQTEKPRGSE 1250
    DDLARHVEEE LGYHFTYPRL LASAITHPSL PSTWGYRVPC YQRLEFLGDS 1300
    LLDMVCVEDL FRRFPDRDPQ WLTEHKMAMV SNKFLGALSV KLGFHRRIMA 1350
    FSNPLQAQIT HYVEEIESAQ AESRGAVDYW VVAKDPPKCL PDMVEAYLGA 1400
    IFVDSKFDFQ VIEVFFERQI KPFFEDMSIY DTFANKHPTT FLHNKLTNEY 1450
    GCTNYCLKAG ELPTIDGAPA GVLAAVIVHG NVISEARSSS SRYAKVKASE 1500
    KALAVLDGLL PFEFCQKYHC GCKETQNSSS AVEIGTAI 1538
    Length:1,538
    Mass (Da):174,902
    Last modified:January 23, 2007 - v1
    Checksum:i22BFCACC1932BB46
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027696 Genomic DNA. Translation: EAW17620.1.
    RefSeqiXP_001259517.1. XM_001259516.1.

    Genome annotation databases

    EnsemblFungiiCADNFIAT00007549; CADNFIAP00007382; CADNFIAG00007549.
    GeneIDi4586299.
    KEGGinfi:NFIA_075500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027696 Genomic DNA. Translation: EAW17620.1 .
    RefSeqi XP_001259517.1. XM_001259516.1.

    3D structure databases

    ProteinModelPortali A1DE13.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 36630.CADNFIAP00007382.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADNFIAT00007549 ; CADNFIAP00007382 ; CADNFIAG00007549 .
    GeneIDi 4586299.
    KEGGi nfi:NFIA_075500.

    Phylogenomic databases

    eggNOGi COG1111.
    HOGENOMi HOG000048448.
    KOi K11592.
    OMAi NERPRIL.
    OrthoDBi EOG76QFRP.

    Family and domain databases

    Gene3Di 1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProi IPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEi PS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

    Entry informationi

    Entry nameiDCL1_NEOFI
    AccessioniPrimary (citable) accession number: A1DE13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3