A1DDZ3 (ALG3_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
EC=2.4.1.258

Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase

Gene names

Name:	alg3
ORF Names:	NFIA_075290

Organism

Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]

Taxonomic identifier

331117 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Neosartorya

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase		PRO_0000350928
Regions
	Topological domain	1 – 18	18	Lumenal Potential
	Transmembrane	19 – 39	21	Helical; Potential
	Topological domain	40 – 69	30	Cytoplasmic Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Topological domain	91 – 95	5	Lumenal Potential
	Transmembrane	96 – 116	21	Helical; Potential
	Topological domain	117 – 139	23	Cytoplasmic Potential
	Transmembrane	140 – 160	21	Helical; Potential
	Topological domain	161 – 181	21	Lumenal Potential
	Transmembrane	182 – 202	21	Helical; Potential
	Transmembrane	203 – 223	21	Helical; Potential
	Topological domain	224 – 261	38	Cytoplasmic Potential
	Transmembrane	262 – 282	21	Helical; Potential
	Topological domain	283 – 306	24	Lumenal Potential
	Transmembrane	307 – 327	21	Helical; Potential
	Topological domain	328 – 340	13	Cytoplasmic Potential
	Transmembrane	341 – 361	21	Helical; Potential
	Topological domain	362 – 375	14	Lumenal Potential
	Transmembrane	376 – 396	21	Helical; Potential
	Topological domain	397 – 411	15	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	A1DDZ3 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 5EB29961244D8EAF	FASTA	411	46,431
	10 20 30 40 50 60 MDLKHTLRDL CMNPRHTRWV APLLILGDAV LCALIIWKVP YTEIDWTTYM QQISLYISGE 70 80 90 100 110 120 RDYTLIKGST GPLVYPAAHV YIFNILYHLT DEGRDIFLGQ ILFAILYLAT LTVAMTCYRQ 130 140 150 160 170 180 AGAPPYLLVP LVLSKRLHSV FMLRLFNDGF AAYAMWVSIL LFMNKKWTAG AIVWSTGVGI 190 200 210 220 230 240 KMTLLLLAPA IAVVLVLSLS LGPSMQLGFL AVLIQVLFGI PFLQNNPAGY VSRAFELTRQ 250 260 270 280 290 300 FMFKWTVNWR FVGEELFLSR KFSLALLALH ILLLGLFVAT VWLKPSGSDL PSFLQRLIQR 310 320 330 340 350 360 RYRTASLSKS FIMTAMLSSL AIGLLCARSL HYQFFAYLAC ATPFLLWQAG FHPILVYVVW 370 380 390 400 410 VAQEWAWNTY PSTNASSLVV ILSLAAQVFG VLGNSFSRKH LDQSSQKEHL Q

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed: 18404212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027696 Genomic DNA. Translation: EAW17600.1.
RefSeq	XP_001259497.1. XM_001259496.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADNFIAT00007144; CADNFIAP00006977; CADNFIAG00007144.
GeneID	4585789.
KEGG	nfi:NFIA_075290.
Phylogenomic databases
GeneTree	EFGT00050000003598.
OrthoDB	EOG4MPN04.
PhylomeDB	A1DDZ3.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
KO	K03845.
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG3_NEOFI

Accession

Primary (citable) accession number: A1DDZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program