A1DBS6 (EGLD_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable endo-beta-1,4-glucanase D
Short name=Endoglucanase D
EC=3.2.1.4

Alternative name(s):
Carboxymethylcellulase D
Cellulase D

Gene names

Name:	eglD
ORF Names:	NFIA_099510

Organism

Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]

Taxonomic identifier

331117 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Neosartorya › Neosartorya fischeri group › Neosartorya fischeri

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Domain	Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM). The genes for catalytic modules and CBMs seem to have evolved separately and have been linked by gene fusion.
Sequence similarities	Belongs to the glycosyl hydrolase 61 family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 348	329	Probable endo-beta-1,4-glucanase D		PRO_0000394068
Regions
	Domain	310 – 346	37	CBM1
	Region	20 – 234	215	Catalytic
	Region	235 – 311	77	Ser/Thr-rich linker
Sites
	Active site	164	1	Proton donor By similarity
	Active site	210	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Disulfide bond	318 ↔ 335		By similarity
	Disulfide bond	329 ↔ 345		By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1DBS6 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 3126BF3D4DC88161	FASTA	348	35,569
	10 20 30 40 50 60 MKSTFGLLAL AAAAKLVSAH ATVHAVWIND VDQGAGNSAD GYIRTPPNNS PITDVTSADM 70 80 90 100 110 120 TCNVNGKNSV AKTLSVKAGD KVTFEWHHDS RSASDDIIAS SHMGPVMVYM APTEKGTAGN 130 140 150 160 170 180 GWVKIAEEGY SNGKWAVANL IANKGKHSIT VPDVPAGEYL LRPEIIALHE GNRQGGAQFY 190 200 210 220 230 240 MECVQVKVTS AGTKTLPAGV SIPGAYKATD PGVLFDMYNS FTSYPIPGPA VWDGSSSGSS 250 260 270 280 290 300 GSSDSSPATT APEASVTSAP TKQGSADTSA SPTTFVTATK PTTTAAPAAP PASTGSNSGS 310 320 330 340 GSSGSGSASG SVKIYQQCGG KNYSGATSCE AGLTCKEWNP YYHQCLKA

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027694 Genomic DNA. Translation: EAW20316.1.
RefSeq	XP_001262213.1. XM_001262212.1.
3D structure databases
ProteinModelPortal	A1DBS6.
SMR	A1DBS6. Positions 312-346.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADNFIAT00009166; CADNFIAP00008952; CADNFIAG00009166.
GeneID	4588579.
KEGG	nfi:NFIA_099510.
Phylogenomic databases
eggNOG	NOG120437.
HOGENOM	HOG000158937.
OrthoDB	EOG44F9K9.
Family and domain databases
InterPro	IPR000254. Cellulose-bd_dom_fun. IPR005103. Glyco_hydro_61. [Graphical view]
Pfam	PF00734. CBM_1. 1 hit. PF03443. Glyco_hydro_61. 1 hit. [Graphical view]
ProDom	PD001821. CBD_fun. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00236. fCBD. 1 hit. [Graphical view]
SUPFAM	SSF57180. CBD_fun. 1 hit.
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EGLD_NEOFI

Accession

Primary (citable) accession number: A1DBS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	January 23, 2007
Last modified:	May 16, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program