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A1DBS6 (EGLD_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable endo-beta-1,4-glucanase D

Short name=Endoglucanase D
EC=3.2.1.4
Alternative name(s):
Carboxymethylcellulase D
Cellulase D
Gene names
Name:eglD
ORF Names:NFIA_099510
OrganismNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]
Taxonomic identifier331117 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates By similarity.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted By similarity.

Domain

Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM). The genes for catalytic modules and CBMs seem to have evolved separately and have been linked by gene fusion.

Sequence similarities

Belongs to the glycosyl hydrolase 61 family.

Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

cellulose binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 348329Probable endo-beta-1,4-glucanase D
PRO_0000394068

Regions

Domain310 – 34637CBM1
Region20 – 234215Catalytic
Region235 – 31177Ser/Thr-rich linker

Sites

Active site1641Proton donor By similarity
Active site2101Nucleophile By similarity

Amino acid modifications

Glycosylation3221N-linked (GlcNAc...) Potential
Disulfide bond318 ↔ 335 By similarity
Disulfide bond329 ↔ 345 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1DBS6 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 3126BF3D4DC88161

FASTA34835,569
        10         20         30         40         50         60 
MKSTFGLLAL AAAAKLVSAH ATVHAVWIND VDQGAGNSAD GYIRTPPNNS PITDVTSADM 

        70         80         90        100        110        120 
TCNVNGKNSV AKTLSVKAGD KVTFEWHHDS RSASDDIIAS SHMGPVMVYM APTEKGTAGN 

       130        140        150        160        170        180 
GWVKIAEEGY SNGKWAVANL IANKGKHSIT VPDVPAGEYL LRPEIIALHE GNRQGGAQFY 

       190        200        210        220        230        240 
MECVQVKVTS AGTKTLPAGV SIPGAYKATD PGVLFDMYNS FTSYPIPGPA VWDGSSSGSS 

       250        260        270        280        290        300 
GSSDSSPATT APEASVTSAP TKQGSADTSA SPTTFVTATK PTTTAAPAAP PASTGSNSGS 

       310        320        330        340 
GSSGSGSASG SVKIYQQCGG KNYSGATSCE AGLTCKEWNP YYHQCLKA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS027694 Genomic DNA. Translation: EAW20316.1.
RefSeqXP_001262213.1. XM_001262212.1.

3D structure databases

ProteinModelPortalA1DBS6.
SMRA1DBS6. Positions 312-346.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADNFIAT00009166; CADNFIAP00008952; CADNFIAG00009166.
GeneID4588579.
KEGGnfi:NFIA_099510.

Phylogenomic databases

eggNOGNOG120437.
HOGENOMHOG000158937.
OMAGYIDSPP.
OrthoDBEOG7KM64H.

Family and domain databases

InterProIPR000254. Cellulose-bd_dom_fun.
IPR005103. Glyco_hydro_61.
[Graphical view]
PfamPF00734. CBM_1. 1 hit.
PF03443. Glyco_hydro_61. 1 hit.
[Graphical view]
ProDomPD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMSSF57180. SSF57180. 1 hit.
PROSITEPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEGLD_NEOFI
AccessionPrimary (citable) accession number: A1DBS6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: January 23, 2007
Last modified: November 13, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries