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Protein

Dicer-like protein 2

Gene

dcl2

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons (By similarity).By similarity

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1137Magnesium or manganeseBy similarity1
Sitei1263Important for activityBy similarity1
Metal bindingi1267Magnesium or manganeseBy similarity1
Metal bindingi1270Magnesium or manganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Helicase, Hydrolase

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dicer-like protein 2
Including the following 2 domains:
Endoribonuclease dcl2 (EC:3.1.26.-)
ATP-dependent helicase dcl2 (EC:3.6.4.-)
Gene namesi
Name:dcl2
ORF Names:NFIA_030600
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006702 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:NFIA_030600.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003067951 – 1388Dicer-like protein 2Add BLAST1388

Structurei

3D structure databases

ProteinModelPortaliA1D9Z6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 203Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST181
Domaini371 – 537Helicase C-terminalPROSITE-ProRule annotationAdd BLAST167
Domaini564 – 658Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST95
Domaini919 – 1059RNase III 1PROSITE-ProRule annotationAdd BLAST141
Domaini1098 – 1281RNase III 2PROSITE-ProRule annotationAdd BLAST184

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi144 – 147DEAH box4

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000048683.
OMAiTRERFRC.
OrthoDBiEOG092C02VR.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1D9Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSVTACQG ASPYQPRNYQ LEMLEASMKE NIIVAMDTGS GKTHIAVLRI
60 70 80 90 100
KAELDICPPD KLVWFLAPTV ALCIQQHEVI ASNLPAVRTR TLTGLDKVEL
110 120 130 140 150
WTEQSIWDAV LNGYRVIVST HAVLADALSH GFVKMSRLAL LIFDEAHHCT
160 170 180 190 200
RRHAANKIMR DFYHPTLTKS GPGAVPRIMG LTASPVVRSN HQELLTVESN
210 220 230 240 250
LDAVCRTPRV HRQELVKFTH RPHLQQIWYT PTDPAGFKSA SQTLGALYHA
260 270 280 290 300
WETLDIGDDP YIQRLRKSPL DDTALKKALL TGKTYCREQL RRFVDRSRHI
310 320 330 340 350
FEELGEWAAE YYIYASIKQL GDRVRNSYMS GDWDEAEKAY LVDFLSKIPA
360 370 380 390 400
SEIQLALNDP GSFRISPKFE SLLNFLDSLD EREFSGLIFV KQRATVSAMT
410 420 430 440 450
SLLSVHPCTR ERFRCAAYVG WSNGSASKDI LGDLLNMQLQ RDTLDDFRSG
460 470 480 490 500
RKNLIIATDV LEEGIDISAC SVVVCYDKPP NLKSFVQRRG RARRKQSTFA
510 520 530 540 550
IMFPTDDASA DVSKWQDLEQ AMIEAYQDDE RQLQSVSALE SLDEEVMERL
560 570 580 590 600
TVESTSAVLT ADMAMAHLHH FCAVLPPQPY ADMRPVFSFE TNEDGLLKGT
610 620 630 640 650
VILPSCVHPK VRRTEGRRWW RTERAAMKET AFQAYKALYE FGLVNDHLLP
660 670 680 690 700
LTKRPELKSH DLGAMPSILE TSEQYDPWIE WAYSWSSPDI HQSRIVVRMN
710 720 730 740 750
EGRGDELCMR LMGPTYLPPL SPMTLFWNNS TTFTVTFEAA ERVPLVPLSS
760 770 780 790 800
VEDMRAITAL YLKATNSRVC SSERDFTALF APDLHHTELK GWLNAYEGCD
810 820 830 840 850
PAMEVYSRGH NPLLMGVVRD HSRYGEPFLF RKWLVSDQNP SCSVVELECA
860 870 880 890 900
PFPHRRNLLH RQRLANSQVD VDEETPESAA KNPIVAADAC TIDRLPFTMA
910 920 930 940 950
IFGLFISAIV ERLETELIAT RLRETILRDV GFKSTDHIIT AISTPFAHAL
960 970 980 990 1000
TNYQRYEFLG DSILKFSVSC QLFFQHPNWH EGYLSEGRAM IVQNPRLAKA
1010 1020 1030 1040 1050
ALDTGLDAYI VTKRIASRKW SAPLISEKLE RVPAKRQMST KVLADVVEAL
1060 1070 1080 1090 1100
IGAAYMDGGH ATAQACIRRL LPEINLHAVD TRTATRSVAP ESARHMMNER
1110 1120 1130 1140 1150
LKDHIGYTFE DESLLVEALT HPSCDYDSTT QSYQRLEYLG DAVLDMVIVS
1160 1170 1180 1190 1200
AIFNHPIQRP QGDMTKIKHA VVNANLLAFL CMESATSEEK LDVAQTSKDS
1210 1220 1230 1240 1250
FAVTTSQESV ELWRFMRYRG QNLNAARDAS LARHRALRDE IASSLLHAPH
1260 1270 1280 1290 1300
YPWHALSRLN ADKFFSDIVE SVLGAIFVDS GGDLAPCEVF VERIGLMAYL
1310 1320 1330 1340 1350
RRILDQEIDV RHPRSVAQQL AKTNIQFVLQ RVPNEEGGAS YQCSVRIEQA
1360 1370 1380
ELFVVTGCLT AEEAEVTAAV EAIKFLTRDE GSTPLNTS
Length:1,388
Mass (Da):156,430
Last modified:January 23, 2007 - v1
Checksum:iC30E206A2EB2D3DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027693 Genomic DNA. Translation: EAW20627.1.
RefSeqiXP_001262524.1. XM_001262523.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00002760; CADNFIAP00002683; CADNFIAG00002760.
GeneIDi4589127.
KEGGinfi:NFIA_030600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027693 Genomic DNA. Translation: EAW20627.1.
RefSeqiXP_001262524.1. XM_001262523.1.

3D structure databases

ProteinModelPortaliA1D9Z6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADNFIAT00002760; CADNFIAP00002683; CADNFIAG00002760.
GeneIDi4589127.
KEGGinfi:NFIA_030600.

Organism-specific databases

EuPathDBiFungiDB:NFIA_030600.

Phylogenomic databases

HOGENOMiHOG000048683.
OMAiTRERFRC.
OrthoDBiEOG092C02VR.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCL2_NEOFI
AccessioniPrimary (citable) accession number: A1D9Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.