Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A1D9Z6 (DCL2_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dicer-like protein 2

Including the following 2 domains:

  1. Endoribonuclease dcl2
    EC=3.1.26.-
  2. ATP-dependent helicase dcl2
    EC=3.6.4.-
Gene names
Name:dcl2
ORF Names:NFIA_030600
OrganismNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]
Taxonomic identifier331117 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya

Protein attributes

Sequence length1388 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.

Cofactor

Magnesium or manganese By similarity.

Sequence similarities

Belongs to the helicase family. Dicer subfamily.

Contains 1 Dicer dsRNA-binding fold domain.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 2 RNase III domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13881388Dicer-like protein 2
PRO_0000306795

Regions

Domain23 – 203181Helicase ATP-binding
Domain371 – 537167Helicase C-terminal
Domain564 – 65895Dicer dsRNA-binding fold
Domain919 – 1059141RNase III 1
Domain1098 – 1281184RNase III 2
Nucleotide binding36 – 438ATP By similarity
Motif144 – 1474DEAH box

Sites

Metal binding11371Magnesium or manganese By similarity
Metal binding12671Magnesium or manganese By similarity
Metal binding12701Magnesium or manganese By similarity
Site12631Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A1D9Z6 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: C30E206A2EB2D3DB

FASTA1,388156,430
        10         20         30         40         50         60 
MASSVTACQG ASPYQPRNYQ LEMLEASMKE NIIVAMDTGS GKTHIAVLRI KAELDICPPD 

        70         80         90        100        110        120 
KLVWFLAPTV ALCIQQHEVI ASNLPAVRTR TLTGLDKVEL WTEQSIWDAV LNGYRVIVST 

       130        140        150        160        170        180 
HAVLADALSH GFVKMSRLAL LIFDEAHHCT RRHAANKIMR DFYHPTLTKS GPGAVPRIMG 

       190        200        210        220        230        240 
LTASPVVRSN HQELLTVESN LDAVCRTPRV HRQELVKFTH RPHLQQIWYT PTDPAGFKSA 

       250        260        270        280        290        300 
SQTLGALYHA WETLDIGDDP YIQRLRKSPL DDTALKKALL TGKTYCREQL RRFVDRSRHI 

       310        320        330        340        350        360 
FEELGEWAAE YYIYASIKQL GDRVRNSYMS GDWDEAEKAY LVDFLSKIPA SEIQLALNDP 

       370        380        390        400        410        420 
GSFRISPKFE SLLNFLDSLD EREFSGLIFV KQRATVSAMT SLLSVHPCTR ERFRCAAYVG 

       430        440        450        460        470        480 
WSNGSASKDI LGDLLNMQLQ RDTLDDFRSG RKNLIIATDV LEEGIDISAC SVVVCYDKPP 

       490        500        510        520        530        540 
NLKSFVQRRG RARRKQSTFA IMFPTDDASA DVSKWQDLEQ AMIEAYQDDE RQLQSVSALE 

       550        560        570        580        590        600 
SLDEEVMERL TVESTSAVLT ADMAMAHLHH FCAVLPPQPY ADMRPVFSFE TNEDGLLKGT 

       610        620        630        640        650        660 
VILPSCVHPK VRRTEGRRWW RTERAAMKET AFQAYKALYE FGLVNDHLLP LTKRPELKSH 

       670        680        690        700        710        720 
DLGAMPSILE TSEQYDPWIE WAYSWSSPDI HQSRIVVRMN EGRGDELCMR LMGPTYLPPL 

       730        740        750        760        770        780 
SPMTLFWNNS TTFTVTFEAA ERVPLVPLSS VEDMRAITAL YLKATNSRVC SSERDFTALF 

       790        800        810        820        830        840 
APDLHHTELK GWLNAYEGCD PAMEVYSRGH NPLLMGVVRD HSRYGEPFLF RKWLVSDQNP 

       850        860        870        880        890        900 
SCSVVELECA PFPHRRNLLH RQRLANSQVD VDEETPESAA KNPIVAADAC TIDRLPFTMA 

       910        920        930        940        950        960 
IFGLFISAIV ERLETELIAT RLRETILRDV GFKSTDHIIT AISTPFAHAL TNYQRYEFLG 

       970        980        990       1000       1010       1020 
DSILKFSVSC QLFFQHPNWH EGYLSEGRAM IVQNPRLAKA ALDTGLDAYI VTKRIASRKW 

      1030       1040       1050       1060       1070       1080 
SAPLISEKLE RVPAKRQMST KVLADVVEAL IGAAYMDGGH ATAQACIRRL LPEINLHAVD 

      1090       1100       1110       1120       1130       1140 
TRTATRSVAP ESARHMMNER LKDHIGYTFE DESLLVEALT HPSCDYDSTT QSYQRLEYLG 

      1150       1160       1170       1180       1190       1200 
DAVLDMVIVS AIFNHPIQRP QGDMTKIKHA VVNANLLAFL CMESATSEEK LDVAQTSKDS 

      1210       1220       1230       1240       1250       1260 
FAVTTSQESV ELWRFMRYRG QNLNAARDAS LARHRALRDE IASSLLHAPH YPWHALSRLN 

      1270       1280       1290       1300       1310       1320 
ADKFFSDIVE SVLGAIFVDS GGDLAPCEVF VERIGLMAYL RRILDQEIDV RHPRSVAQQL 

      1330       1340       1350       1360       1370       1380 
AKTNIQFVLQ RVPNEEGGAS YQCSVRIEQA ELFVVTGCLT AEEAEVTAAV EAIKFLTRDE 


GSTPLNTS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS027693 Genomic DNA. Translation: EAW20627.1.
RefSeqXP_001262524.1. XM_001262523.1.

3D structure databases

ProteinModelPortalA1D9Z6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING36630.CADNFIAP00002683.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADNFIAT00002760; CADNFIAP00002683; CADNFIAG00002760.
GeneID4589127.
KEGGnfi:NFIA_030600.

Phylogenomic databases

eggNOGCOG1111.
HOGENOMHOG000048683.
OMAELECAPF.
OrthoDBEOG76QFRP.

Family and domain databases

Gene3D1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProIPR005034. Dicer_dimerisation_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDCL2_NEOFI
AccessionPrimary (citable) accession number: A1D9Z6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families