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A1D9Z6

- DCL2_NEOFI

UniProt

A1D9Z6 - DCL2_NEOFI

Protein

Dicer-like protein 2

Gene

dcl2

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.By similarity

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1137 – 11371Magnesium or manganeseBy similarity
    Sitei1263 – 12631Important for activityBy similarity
    Metal bindingi1267 – 12671Magnesium or manganeseBy similarity
    Metal bindingi1270 – 12701Magnesium or manganeseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi36 – 438ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. ribonuclease III activity Source: InterPro
    5. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. regulation of defense response to virus Source: UniProtKB-KW
    3. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Antiviral protein, Helicase, Hydrolase

    Keywords - Biological processi

    Antiviral defense

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dicer-like protein 2
    Including the following 2 domains:
    Endoribonuclease dcl2 (EC:3.1.26.-)
    ATP-dependent helicase dcl2 (EC:3.6.4.-)
    Gene namesi
    Name:dcl2
    ORF Names:NFIA_030600
    OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
    Taxonomic identifieri331117 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
    ProteomesiUP000006702: Unassembled WGS sequence

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13881388Dicer-like protein 2PRO_0000306795Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi36630.CADNFIAP00002683.

    Structurei

    3D structure databases

    ProteinModelPortaliA1D9Z6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 203181Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini371 – 537167Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini564 – 65895Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
    BLAST
    Domaini919 – 1059141RNase III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1098 – 1281184RNase III 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi144 – 1474DEAH box

    Sequence similaritiesi

    Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
    Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 2 RNase III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1111.
    HOGENOMiHOG000048683.
    OMAiELECAPF.
    OrthoDBiEOG76QFRP.

    Family and domain databases

    Gene3Di1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProiIPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEiPS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1D9Z6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASSVTACQG ASPYQPRNYQ LEMLEASMKE NIIVAMDTGS GKTHIAVLRI     50
    KAELDICPPD KLVWFLAPTV ALCIQQHEVI ASNLPAVRTR TLTGLDKVEL 100
    WTEQSIWDAV LNGYRVIVST HAVLADALSH GFVKMSRLAL LIFDEAHHCT 150
    RRHAANKIMR DFYHPTLTKS GPGAVPRIMG LTASPVVRSN HQELLTVESN 200
    LDAVCRTPRV HRQELVKFTH RPHLQQIWYT PTDPAGFKSA SQTLGALYHA 250
    WETLDIGDDP YIQRLRKSPL DDTALKKALL TGKTYCREQL RRFVDRSRHI 300
    FEELGEWAAE YYIYASIKQL GDRVRNSYMS GDWDEAEKAY LVDFLSKIPA 350
    SEIQLALNDP GSFRISPKFE SLLNFLDSLD EREFSGLIFV KQRATVSAMT 400
    SLLSVHPCTR ERFRCAAYVG WSNGSASKDI LGDLLNMQLQ RDTLDDFRSG 450
    RKNLIIATDV LEEGIDISAC SVVVCYDKPP NLKSFVQRRG RARRKQSTFA 500
    IMFPTDDASA DVSKWQDLEQ AMIEAYQDDE RQLQSVSALE SLDEEVMERL 550
    TVESTSAVLT ADMAMAHLHH FCAVLPPQPY ADMRPVFSFE TNEDGLLKGT 600
    VILPSCVHPK VRRTEGRRWW RTERAAMKET AFQAYKALYE FGLVNDHLLP 650
    LTKRPELKSH DLGAMPSILE TSEQYDPWIE WAYSWSSPDI HQSRIVVRMN 700
    EGRGDELCMR LMGPTYLPPL SPMTLFWNNS TTFTVTFEAA ERVPLVPLSS 750
    VEDMRAITAL YLKATNSRVC SSERDFTALF APDLHHTELK GWLNAYEGCD 800
    PAMEVYSRGH NPLLMGVVRD HSRYGEPFLF RKWLVSDQNP SCSVVELECA 850
    PFPHRRNLLH RQRLANSQVD VDEETPESAA KNPIVAADAC TIDRLPFTMA 900
    IFGLFISAIV ERLETELIAT RLRETILRDV GFKSTDHIIT AISTPFAHAL 950
    TNYQRYEFLG DSILKFSVSC QLFFQHPNWH EGYLSEGRAM IVQNPRLAKA 1000
    ALDTGLDAYI VTKRIASRKW SAPLISEKLE RVPAKRQMST KVLADVVEAL 1050
    IGAAYMDGGH ATAQACIRRL LPEINLHAVD TRTATRSVAP ESARHMMNER 1100
    LKDHIGYTFE DESLLVEALT HPSCDYDSTT QSYQRLEYLG DAVLDMVIVS 1150
    AIFNHPIQRP QGDMTKIKHA VVNANLLAFL CMESATSEEK LDVAQTSKDS 1200
    FAVTTSQESV ELWRFMRYRG QNLNAARDAS LARHRALRDE IASSLLHAPH 1250
    YPWHALSRLN ADKFFSDIVE SVLGAIFVDS GGDLAPCEVF VERIGLMAYL 1300
    RRILDQEIDV RHPRSVAQQL AKTNIQFVLQ RVPNEEGGAS YQCSVRIEQA 1350
    ELFVVTGCLT AEEAEVTAAV EAIKFLTRDE GSTPLNTS 1388
    Length:1,388
    Mass (Da):156,430
    Last modified:January 23, 2007 - v1
    Checksum:iC30E206A2EB2D3DB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027693 Genomic DNA. Translation: EAW20627.1.
    RefSeqiXP_001262524.1. XM_001262523.1.

    Genome annotation databases

    EnsemblFungiiCADNFIAT00002760; CADNFIAP00002683; CADNFIAG00002760.
    GeneIDi4589127.
    KEGGinfi:NFIA_030600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027693 Genomic DNA. Translation: EAW20627.1 .
    RefSeqi XP_001262524.1. XM_001262523.1.

    3D structure databases

    ProteinModelPortali A1D9Z6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 36630.CADNFIAP00002683.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADNFIAT00002760 ; CADNFIAP00002683 ; CADNFIAG00002760 .
    GeneIDi 4589127.
    KEGGi nfi:NFIA_030600.

    Phylogenomic databases

    eggNOGi COG1111.
    HOGENOMi HOG000048683.
    OMAi ELECAPF.
    OrthoDBi EOG76QFRP.

    Family and domain databases

    Gene3Di 1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProi IPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEi PS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

    Entry informationi

    Entry nameiDCL2_NEOFI
    AccessioniPrimary (citable) accession number: A1D9Z6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3