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A1D9Z6

- DCL2_NEOFI

UniProt

A1D9Z6 - DCL2_NEOFI

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Protein

Dicer-like protein 2

Gene

dcl2

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons (By similarity).By similarity

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1137 – 11371Magnesium or manganeseBy similarity
Sitei1263 – 12631Important for activityBy similarity
Metal bindingi1267 – 12671Magnesium or manganeseBy similarity
Metal bindingi1270 – 12701Magnesium or manganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 438ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. helicase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. ribonuclease III activity Source: InterPro
  5. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
  2. regulation of defense response to virus Source: UniProtKB-KW
  3. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Helicase, Hydrolase

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dicer-like protein 2
Including the following 2 domains:
Endoribonuclease dcl2 (EC:3.1.26.-)
ATP-dependent helicase dcl2 (EC:3.6.4.-)
Gene namesi
Name:dcl2
ORF Names:NFIA_030600
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
ProteomesiUP000006702: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13881388Dicer-like protein 2PRO_0000306795Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi36630.CADNFIAP00002683.

Structurei

3D structure databases

ProteinModelPortaliA1D9Z6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 203181Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini371 – 537167Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini564 – 65895Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
BLAST
Domaini919 – 1059141RNase III 1PROSITE-ProRule annotationAdd
BLAST
Domaini1098 – 1281184RNase III 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi144 – 1474DEAH box

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1111.
HOGENOMiHOG000048683.
OMAiELECAPF.
OrthoDBiEOG76QFRP.

Family and domain databases

Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1D9Z6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSVTACQG ASPYQPRNYQ LEMLEASMKE NIIVAMDTGS GKTHIAVLRI
60 70 80 90 100
KAELDICPPD KLVWFLAPTV ALCIQQHEVI ASNLPAVRTR TLTGLDKVEL
110 120 130 140 150
WTEQSIWDAV LNGYRVIVST HAVLADALSH GFVKMSRLAL LIFDEAHHCT
160 170 180 190 200
RRHAANKIMR DFYHPTLTKS GPGAVPRIMG LTASPVVRSN HQELLTVESN
210 220 230 240 250
LDAVCRTPRV HRQELVKFTH RPHLQQIWYT PTDPAGFKSA SQTLGALYHA
260 270 280 290 300
WETLDIGDDP YIQRLRKSPL DDTALKKALL TGKTYCREQL RRFVDRSRHI
310 320 330 340 350
FEELGEWAAE YYIYASIKQL GDRVRNSYMS GDWDEAEKAY LVDFLSKIPA
360 370 380 390 400
SEIQLALNDP GSFRISPKFE SLLNFLDSLD EREFSGLIFV KQRATVSAMT
410 420 430 440 450
SLLSVHPCTR ERFRCAAYVG WSNGSASKDI LGDLLNMQLQ RDTLDDFRSG
460 470 480 490 500
RKNLIIATDV LEEGIDISAC SVVVCYDKPP NLKSFVQRRG RARRKQSTFA
510 520 530 540 550
IMFPTDDASA DVSKWQDLEQ AMIEAYQDDE RQLQSVSALE SLDEEVMERL
560 570 580 590 600
TVESTSAVLT ADMAMAHLHH FCAVLPPQPY ADMRPVFSFE TNEDGLLKGT
610 620 630 640 650
VILPSCVHPK VRRTEGRRWW RTERAAMKET AFQAYKALYE FGLVNDHLLP
660 670 680 690 700
LTKRPELKSH DLGAMPSILE TSEQYDPWIE WAYSWSSPDI HQSRIVVRMN
710 720 730 740 750
EGRGDELCMR LMGPTYLPPL SPMTLFWNNS TTFTVTFEAA ERVPLVPLSS
760 770 780 790 800
VEDMRAITAL YLKATNSRVC SSERDFTALF APDLHHTELK GWLNAYEGCD
810 820 830 840 850
PAMEVYSRGH NPLLMGVVRD HSRYGEPFLF RKWLVSDQNP SCSVVELECA
860 870 880 890 900
PFPHRRNLLH RQRLANSQVD VDEETPESAA KNPIVAADAC TIDRLPFTMA
910 920 930 940 950
IFGLFISAIV ERLETELIAT RLRETILRDV GFKSTDHIIT AISTPFAHAL
960 970 980 990 1000
TNYQRYEFLG DSILKFSVSC QLFFQHPNWH EGYLSEGRAM IVQNPRLAKA
1010 1020 1030 1040 1050
ALDTGLDAYI VTKRIASRKW SAPLISEKLE RVPAKRQMST KVLADVVEAL
1060 1070 1080 1090 1100
IGAAYMDGGH ATAQACIRRL LPEINLHAVD TRTATRSVAP ESARHMMNER
1110 1120 1130 1140 1150
LKDHIGYTFE DESLLVEALT HPSCDYDSTT QSYQRLEYLG DAVLDMVIVS
1160 1170 1180 1190 1200
AIFNHPIQRP QGDMTKIKHA VVNANLLAFL CMESATSEEK LDVAQTSKDS
1210 1220 1230 1240 1250
FAVTTSQESV ELWRFMRYRG QNLNAARDAS LARHRALRDE IASSLLHAPH
1260 1270 1280 1290 1300
YPWHALSRLN ADKFFSDIVE SVLGAIFVDS GGDLAPCEVF VERIGLMAYL
1310 1320 1330 1340 1350
RRILDQEIDV RHPRSVAQQL AKTNIQFVLQ RVPNEEGGAS YQCSVRIEQA
1360 1370 1380
ELFVVTGCLT AEEAEVTAAV EAIKFLTRDE GSTPLNTS
Length:1,388
Mass (Da):156,430
Last modified:January 23, 2007 - v1
Checksum:iC30E206A2EB2D3DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027693 Genomic DNA. Translation: EAW20627.1.
RefSeqiXP_001262524.1. XM_001262523.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00002760; CADNFIAP00002683; CADNFIAG00002760.
GeneIDi4589127.
KEGGinfi:NFIA_030600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027693 Genomic DNA. Translation: EAW20627.1 .
RefSeqi XP_001262524.1. XM_001262523.1.

3D structure databases

ProteinModelPortali A1D9Z6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 36630.CADNFIAP00002683.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADNFIAT00002760 ; CADNFIAP00002683 ; CADNFIAG00002760 .
GeneIDi 4589127.
KEGGi nfi:NFIA_030600.

Phylogenomic databases

eggNOGi COG1111.
HOGENOMi HOG000048683.
OMAi ELECAPF.
OrthoDBi EOG76QFRP.

Family and domain databases

Gene3Di 1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProi IPR011545. DEAD/DEAH_box_helicase_dom.
IPR005034. Dicer_dimerisation_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEi PS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Entry informationi

Entry nameiDCL2_NEOFI
AccessioniPrimary (citable) accession number: A1D9Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3