A1D932 (CELB_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable endo-beta-1,4-glucanase celB
Short name=Endoglucanase celB
EC=3.2.1.4

Alternative name(s):
Carboxymethylcellulase celB
Cellulase B

Gene names

Name:	celB
ORF Names:	NFIA_114250

Organism

Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]

Taxonomic identifier

331117 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Neosartorya › Neosartorya fischeri group › Neosartorya fischeri

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 7 (cellulase C) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 407	389	Probable endo-beta-1,4-glucanase celB		PRO_0000395160
Sites
	Active site	216	1	Nucleophile By similarity
	Active site	221	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	136	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A1D932 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 022FA57ED78BA1D0	FASTA	407	43,421
	10 20 30 40 50 60 MALTLAATAL VLLPLVTAQQ IGSIAENHPE LTTYRCSSQA GCVAQSTSVV LDINAHWIHQ 70 80 90 100 110 120 NGAQTSCTTS SGLDPSLCPD KVTCSQNCVV EGITDYSSFG VQNSGDAMTL RQYQVQNGQI 130 140 150 160 170 180 KTLRPRVYLL AEDGINYSKL QLLNQEFTFD VDASKLPCGM NGALYLSEMD ASGGRSALNP 190 200 210 220 230 240 AGATYGTGYC DAQCFNPGPW INGEANTAGA GACCQEMDLW EANSRSTIFS PHPCTTAGLY 250 260 270 280 290 300 ACTGAECYSI CDGYGCTYNP YELGAKDYYG YGLTIDTAKP ITVVTQFMTA DNTATGTLAE 310 320 330 340 350 360 IRRLYVQDGK VIGNTAVAMT EAFCSSSRTF EELGGLQRMG EALGRGMVPV FSIWDDPGLW 370 380 390 400 MHWLDSDGAG PCGNTEGDPA FIQANYPNTA VTFSKVRWGD IGSTYSS

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027692 Genomic DNA. Translation: EAW20893.1.
RefSeq	XP_001262790.1. XM_001262789.1.
3D structure databases
ProteinModelPortal	A1D932.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADNFIAT00010428; CADNFIAP00010152; CADNFIAG00010428.
GeneID	4589309.
KEGG	nfi:NFIA_114250.
Phylogenomic databases
eggNOG	NOG138370.
HOGENOM	HOG000182210.
OrthoDB	EOG4XH37H.
Family and domain databases
Gene3D	2.70.100.10. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001722. Glyco_hydro_7. [Graphical view]
Pfam	PF00840. Glyco_hydro_7. 1 hit. [Graphical view]
PRINTS	PR00734. GLHYDRLASE7.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CELB_NEOFI

Accession

Primary (citable) accession number: A1D932

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	January 23, 2007
Last modified:	March 6, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program