Probable mannan endo-1,4-beta-mannosidase A precursor - Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Probable mannan endo-1,4-beta-mannosidase A
EC=3.2.1.78

Alternative name(s):
Endo-beta-1,4-mannanase A

Gene names

Name:	manA
Synonyms:	man1
ORF Names:	NFIA_113780

Organism

Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]

Taxonomic identifier

331117 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Neosartorya ›

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans By similarity.
Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 373

354

Probable mannan endo-1,4-beta-mannosidase A

PRO_0000393707

Sites

Active site

192

1

Proton donor By similarity

Active site

300

1

Nucleophile By similarity

Amino acid modifications

Glycosylation

249

1

N-linked (GlcNAc...) Potential

Glycosylation

320

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

A1D8Y6 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 752634795B064B08
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FASTA

373

41,498

        10         20         30         40         50         60 
MKFSFLTVAS FLIGQVALAA LSAKKFASAS DLQFTIDGKT GYFAGSNSYW IGFLTNNADV 

        70         80         90        100        110        120 
DLVFDHMKES GLKILRVWGF NDVNTVPGQG TVYYQVHANG KSTINTGADG LQRLDYVVHS 

       130        140        150        160        170        180 
AEKRGIKLII NFVNNWDDYG GMNAYVKAYG ETDHNAFYSN KNIQNAYRRY VKAVVSRYTN 

       190        200        210        220        230        240 
SPAVFAWELA NEPRCKGCDT EVLYEWIKST SEYIKKLDKR HMVCIGDEGF GLDLLSDGSY 

       250        260        270        280        290        300 
PYTYVEGSNF TRNLAIPTID FGTFHLYPDS WGTTHEWGNG WAQSHGAACK AAGKPCLFEE 

       310        320        330        340        350        360 
YGVTSNHCAL ETPWQKTSLN TTGVSGDLYW QYGDTLSTGQ SPNDGNTIYY GTDEFKCIVK 

       370 
DHVAAIKAKQ GWV

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References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus."
Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.

Nierman W.C.
PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027692 Genomic DNA. Translation: EAW20847.1.
RefSeq	XP_001262744.1. XM_001262743.1.
3D structure databases
ProteinModelPortal	A1D8Y6.
SMR	A1D8Y6. Positions 24-366.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADNFIAT00010693; CADNFIAP00010417; CADNFIAG00010693.
GeneID	4589380.
KEGG	nfi:NFIA_113780.
Phylogenomic databases
eggNOG	COG3934.
HOGENOM	HOG000169951.
OrthoDB	EOG44BF9Z.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANA_NEOFI

Accession

Primary (citable) accession number: A1D8Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families