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Protein

Probable mannan endo-1,4-beta-mannosidase A

Gene

manA

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei192 – 1921Proton donorBy similarity
Active sitei300 – 3001NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannan endo-1,4-beta-mannosidase A (EC:3.2.1.78)
Alternative name(s):
Endo-beta-1,4-mannanase A
Gene namesi
Name:manA
Synonyms:man1
ORF Names:NFIA_113780
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
ProteomesiUP000006702 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:NFIA_113780.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 373354Probable mannan endo-1,4-beta-mannosidase APRO_0000393707Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliA1D8Y6.
SMRiA1D8Y6. Positions 24-366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3934.
HOGENOMiHOG000169951.
OMAiQFNIDGK.
OrthoDBiEOG7M3J90.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1D8Y6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSFLTVAS FLIGQVALAA LSAKKFASAS DLQFTIDGKT GYFAGSNSYW
60 70 80 90 100
IGFLTNNADV DLVFDHMKES GLKILRVWGF NDVNTVPGQG TVYYQVHANG
110 120 130 140 150
KSTINTGADG LQRLDYVVHS AEKRGIKLII NFVNNWDDYG GMNAYVKAYG
160 170 180 190 200
ETDHNAFYSN KNIQNAYRRY VKAVVSRYTN SPAVFAWELA NEPRCKGCDT
210 220 230 240 250
EVLYEWIKST SEYIKKLDKR HMVCIGDEGF GLDLLSDGSY PYTYVEGSNF
260 270 280 290 300
TRNLAIPTID FGTFHLYPDS WGTTHEWGNG WAQSHGAACK AAGKPCLFEE
310 320 330 340 350
YGVTSNHCAL ETPWQKTSLN TTGVSGDLYW QYGDTLSTGQ SPNDGNTIYY
360 370
GTDEFKCIVK DHVAAIKAKQ GWV
Length:373
Mass (Da):41,498
Last modified:January 23, 2007 - v1
Checksum:i752634795B064B08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027692 Genomic DNA. Translation: EAW20847.1.
RefSeqiXP_001262744.1. XM_001262743.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00010693; CADNFIAP00010417; CADNFIAG00010693.
GeneIDi4589380.
KEGGinfi:NFIA_113780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027692 Genomic DNA. Translation: EAW20847.1.
RefSeqiXP_001262744.1. XM_001262743.1.

3D structure databases

ProteinModelPortaliA1D8Y6.
SMRiA1D8Y6. Positions 24-366.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADNFIAT00010693; CADNFIAP00010417; CADNFIAG00010693.
GeneIDi4589380.
KEGGinfi:NFIA_113780.

Organism-specific databases

EuPathDBiFungiDB:NFIA_113780.

Phylogenomic databases

eggNOGiCOG3934.
HOGENOMiHOG000169951.
OMAiQFNIDGK.
OrthoDBiEOG7M3J90.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Entry informationi

Entry nameiMANA_NEOFI
AccessioniPrimary (citable) accession number: A1D8Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.