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Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei141Substrate; via amide nitrogenBy similarity1
Binding sitei142SubstrateBy similarity1
Binding sitei199SubstrateBy similarity1
Active sitei200Proton donorSequence analysis1
Binding sitei260SubstrateBy similarity1
Active sitei298NucleophileSequence analysis1
Binding sitei364SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase A (EC:3.2.1.23)
Alternative name(s):
Lactase A
Gene namesi
Name:lacA
ORF Names:NFIA_011250
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006702 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:NFIA_011250.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000039522119 – 1006Probable beta-galactosidase AAdd BLAST988

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi156N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi205 ↔ 206By similarity
Glycosylationi207N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi266 ↔ 315By similarity
Glycosylationi373N-linked (GlcNAc...)Sequence analysis1
Glycosylationi402N-linked (GlcNAc...)Sequence analysis1
Glycosylationi422N-linked (GlcNAc...)Sequence analysis1
Glycosylationi622N-linked (GlcNAc...)Sequence analysis1
Glycosylationi777N-linked (GlcNAc...)Sequence analysis1
Glycosylationi914N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliA1D1Z9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922.
OMAiHNAPGTG.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1D1Z9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLSVCAVA LLAAQAAGAS IKHKLNGFTI MEHSDPAKRE LLQKYVTWDE
60 70 80 90 100
KSLFVNGERI MIFSGEVHPF RLPVPSLWLD VFQKIKALGF NCVSFYVDWA
110 120 130 140 150
LLEGKPGKYR AEGNFALEPF FDAAKQAGIY LLARPGPYIN AEASGGGFPG
160 170 180 190 200
WLQRVNGTLR TSDPAYLKAT DNYIAHVAAT VAKGQITNGG PVILYQPENE
210 220 230 240 250
YSGACCNATF PDGDYMQYVI DQARNAGIVV PLINNDAWTG GHNAPGTGKG
260 270 280 290 300
EVDIYGHDSY PLGFDCGHPS VWPKGNLPTT FRTDHLRESP TTPYSLIEFQ
310 320 330 340 350
AGSFDPWGGP GFAACAALVN HEFERVFYKN DLSFGAAILN LYMTFGGTNW
360 370 380 390 400
GNLGHPGGYT SYDYGSPLTE SRNVTREKYS ELKLIGNFVK ASPSYLLATP
410 420 430 440 450
GNLTTSGYAD TADLTVTPLL GNGTGSYFVV RHTDYTSQAS TPYKLSLPTS
460 470 480 490 500
AGRLTVPQLG GTLTLNGRDS KVHVVDYNVA GTNILYSTAE VFTWKKFGDS
510 520 530 540 550
KVLVLYGGPG EHHELAVSLK SDVQVVEGSN SEFTSKKVED VVVVAWDVSA
560 570 580 590 600
SRRIVQIGDL KIFLLDRNSA YNYWVPQLDK DDSSTGYSSE KTTASSIIVK
610 620 630 640 650
AGYLVRTAYT KGSGLYLTAD FNATTPVEVI GAPSNVRNLY INGEKTQFKT
660 670 680 690 700
DKNGIWSTGV KYSAPKIKLP SMKDLDWKYL DTLPEVQSTY DDSAWPAADL
710 720 730 740 750
DTTPNTLRPL TMPKSLHSSD YGFHTGYLIY RGHFVADGSE TTFDVRTQGG
760 770 780 790 800
SAFGSSVWLN EAFLGSWTGL NANADYNSTY RLPQVEKGKN YVLTVVIDTM
810 820 830 840 850
GLNENWVVGT DEMKNPRGIL SYKLSGRDAS AITWKLTGNL GGEDYQDKIR
860 870 880 890 900
GPLNEGGLYA ERQGFHQPEP PSKKWKSASP LDGLSKPGIG FYTAQFDLDI
910 920 930 940 950
PSGWDVPLYF NFGNSTKSAY RVQLYVNGYQ YGKFVSNIGP QTSFPVPQGI
960 970 980 990 1000
LNYQGTNWVA LTLWALESDG AKLDDFELVN TTPVMTALSK IRPSKQPNYR

QRKGAY
Length:1,006
Mass (Da):110,230
Last modified:January 23, 2007 - v1
Checksum:i0F6D74409FAE7B19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027688 Genomic DNA. Translation: EAW22442.1.
RefSeqiXP_001264339.1. XM_001264338.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00001802; CADNFIAP00001763; CADNFIAG00001802.
GeneIDi4591834.
KEGGinfi:NFIA_011250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027688 Genomic DNA. Translation: EAW22442.1.
RefSeqiXP_001264339.1. XM_001264338.1.

3D structure databases

ProteinModelPortaliA1D1Z9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADNFIAT00001802; CADNFIAP00001763; CADNFIAG00001802.
GeneIDi4591834.
KEGGinfi:NFIA_011250.

Organism-specific databases

EuPathDBiFungiDB:NFIA_011250.

Phylogenomic databases

HOGENOMiHOG000181922.
OMAiHNAPGTG.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGALA_NEOFI
AccessioniPrimary (citable) accession number: A1D1Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.