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A1D1W1 (MNS1B_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

EC=3.2.1.113
Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B
Gene names
Name:mns1B
Synonyms:msdS
ORF Names:NFIA_010860
OrganismNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]
Taxonomic identifier331117 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 By similarity.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactor

Ca2+. Can also use Mg2+, but with lower efficiency By similarity.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasmic vesicle lumen By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasmic vesicle
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotein glycosylation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasmic membrane-bounded vesicle lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

mannosyl-oligosaccharide 1,2-alpha-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 493475Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
PRO_0000394824

Sites

Active site3671Proton donor By similarity

Amino acid modifications

Glycosylation871N-linked (GlcNAc...) Potential
Glycosylation1741N-linked (GlcNAc...) Potential
Glycosylation4891N-linked (GlcNAc...) Potential
Disulfide bond324 ↔ 353 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1D1W1 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 8E6C4E050EA8E4CB

FASTA49353,855
        10         20         30         40         50         60 
MHLPSLSVAL ALVSSSLALP QTVLPESDVS SHAAAVKEAF SHAWDGYMKY AFPHDELLPI 

        70         80         90        100        110        120 
SNSYGDSRNG WGASAVDALS TAIVMRNATI VNQILDHIAK IDYSKTSDTV SLFETTIRYL 

       130        140        150        160        170        180 
GGMLSGYDLL KGPAADLVKD STKVDMLLQQ SKNLGDVLKF AFDTPSGVPY NNINITSHGN 

       190        200        210        220        230        240 
DGATTNGLAV TGTLVLEWTR LSDLTGDQEY AKLSQRAESY LLAPQPSSGE PFPGLVGSEI 

       250        260        270        280        290        300 
SIQTGQFTNG FVSWNGGSDS FYEYLMKMYV YDPKRFATYK DRWVAAAESS IDHLASSPAS 

       310        320        330        340        350        360 
RPDLTFLATY NKGSLGLSSQ HLACFDGGSY LLGGTVLDRA DLIDFGLKLV DGCAETYHQT 

       370        380        390        400        410        420 
LTGIGPESFG WDEKSVPADQ KEFYERAGFY VQSGAYILRP EVIESFYYAY RVTGKKQYRD 

       430        440        450        460        470        480 
WVWNAFVNIN KYCRTGSGFA GLTDVNAVNG GNRYDNQESF LFAEVMKYAY LTHAPGMSPI 

       490 
SIADEDKANE SRG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS027688 Genomic DNA. Translation: EAW22404.1.
RefSeqXP_001264301.1. XM_001264300.1.

3D structure databases

ProteinModelPortalA1D1W1.
SMRA1D1W1. Positions 31-474.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING36630.CADNFIAP00001081.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADNFIAT00001120; CADNFIAP00001081; CADNFIAG00001120.
GeneID4591109.
KEGGnfi:NFIA_010860.

Phylogenomic databases

eggNOGNOG300315.
HOGENOMHOG000181987.
KOK01230.
OMAGPESFGW.
OrthoDBEOG7BP8BH.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

Gene3D1.50.10.50. 1 hit.
InterProIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERPTHR11742. PTHR11742. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. SSF48225. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNS1B_NEOFI
AccessionPrimary (citable) accession number: A1D1W1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: January 23, 2007
Last modified: November 13, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries