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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901SubstrateBy similarity
Binding sitei135 – 1351SubstrateBy similarity
Binding sitei136 – 1361Substrate; via amide nitrogenBy similarity
Binding sitei137 – 1371SubstrateBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Active sitei196 – 1961Proton donorSequence Analysis
Binding sitei265 – 2651SubstrateBy similarity
Active sitei308 – 3081NucleophileSequence Analysis
Binding sitei373 – 3731SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:NFIA_008690
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
ProteomesiUP000006702 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:NFIA_008690.

Subcellular locationi

  1. Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 1015994Probable beta-galactosidase BPRO_0000395230Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi271 ↔ 324By similarity
Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi554 – 5541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi679 – 6791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi735 – 7351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi775 – 7751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi821 – 8211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi878 – 8781N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi36630.CADNFIAP00001723.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
OMAiNPVLYVD.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1D199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHIYRLLLL LLSNLWFSAA AQNQSETEWP LHDNGLSKVV QWDHYSFQVN
60 70 80 90 100
GQRIFIFSGE FHYWRIPVPE LWRDILEKVK ATGFTAFAFY SSWAYHAPNN
110 120 130 140 150
RTVDFSTGAR DITPIFELAK ELGMYMIVRP GPYVNAEASA GGFPLWLTTG
160 170 180 190 200
EYGSLRNDDP RYTAAWTPYF ANMSQITSKY QVTDGHNTLV YQIENEYGQQ
210 220 230 240 250
WIGDPKDRNP NKTAVAYMEL LEASALENGI TVPLTSNDPN MNSKSWGSDW
260 270 280 290 300
SNAGGNVDVA GLDSYPSCWT CDVSQCTSTN GEYVPYKVID YYDYFQEVQP
310 320 330 340 350
TLPSFMPEFQ GGSYNPWAGP EGGCPQDTGA EFANLFYRWN IGQRVTAMSL
360 370 380 390 400
YMLYGGTNWG AIAAPVTATS YDYSAPISED RSIGAKYSET KLLALFTRTA
410 420 430 440 450
KDLTMTEAIG NGTQYTTNTA VRAFELRNPQ TNAGFYVTFH NDTTVGGNQA
460 470 480 490 500
FKLHVNTSVG ALTVPKNEGV IQLNGHQSKI IVTDFTLGKR TLLYSTAEVL
510 520 530 540 550
TYAVFENRPT LVLWVPTGES GEFAIKGTKS GKVENGDGCS GINFKREKDY
560 570 580 590 600
LVVNFSQAKG LSVLRLDNGV RVVLLDKAAA YRFWAPALTD DPIVQETETV
610 620 630 640 650
LVHGPYLVRS ASVSKSTLAL RGDSVEKTTL EIFAPHSVRK ITWNGKEVKT
660 670 680 690 700
SQTPYGSLKA TLAAPPTIKL PALTSWRSND SLPERLPSYD DSGPAWIEAN
710 720 730 740 750
HMTTSNPSPP ATLPVLYADE YGFHNGVRLW RGYFNGSASG VFLNIQGGSA
760 770 780 790 800
FGWSAWLNGH FLDSHLGTAT TSQANKTLTF SSSILNPTEN VLLIVHDDTG
810 820 830 840 850
HDQTTGALNP RGIIEARLLS NDTSSPAPGF TQWRIAGTAG GESNLDPIRG
860 870 880 890 900
VFNEDGLFAE RMGWHLPGFD DSAWTPENST TSASSALSFT GATVRFFRTV
910 920 930 940 950
VPLDIPAGLD VSISFVLSTP SAAPKGYRAQ LFVNGYQYGR YNPHIGNQVV
960 970 980 990 1000
FPVPPGILDY QGDNTIGLAV WAQTEEGAGI QVDWKVNYVA DSSLSVAGFG
1010
KGLRPGWTEE RLKFA
Length:1,015
Mass (Da):111,471
Last modified:January 23, 2007 - v1
Checksum:i7BDC616E2D3A2DFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027688 Genomic DNA. Translation: EAW22192.1.
RefSeqiXP_001264089.1. XM_001264088.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00001762; CADNFIAP00001723; CADNFIAG00001762.
GeneIDi4591479.
KEGGinfi:NFIA_008690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027688 Genomic DNA. Translation: EAW22192.1.
RefSeqiXP_001264089.1. XM_001264088.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi36630.CADNFIAP00001723.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADNFIAT00001762; CADNFIAP00001723; CADNFIAG00001762.
GeneIDi4591479.
KEGGinfi:NFIA_008690.

Organism-specific databases

EuPathDBiFungiDB:NFIA_008690.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
OMAiNPVLYVD.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Entry informationi

Entry nameiBGALB_NEOFI
AccessioniPrimary (citable) accession number: A1D199
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.