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Protein

Probable pectin lyase A

Gene

pelA

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Pectinolytic enzymes consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal glycosidic bonds of highly methylated pectins (By similarity).By similarity

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei254 – 2541Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectin lyase A (EC:4.2.2.10)
Short name:
PLA
Gene namesi
Name:pelA
ORF Names:NFIA_033080
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006702 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:NFIA_033080.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 378360Probable pectin lyase APRO_0000394344Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi81 ↔ 100By similarity
Disulfide bondi90 ↔ 224By similarity
Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence analysis
Disulfide bondi321 ↔ 329By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide lyase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000175137.
KOiK01732.
OMAiSATCNGR.
OrthoDBiEOG092C2XJ9.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1CYC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYQGLLAIA GCIASASAVS VSGAAEGFAK GVTGGGSATA VYPSTTAELV
60 70 80 90 100
SYLGDSEARV IVLTKTFDFT GTEGTTTATG CAPWGTASAC QLAINQNDWC
110 120 130 140 150
TNYEPDAPSV SVTYDNAGTL GITVKSNKSL LGSGSSGVIK GKGLRIVSGA
160 170 180 190 200
SNIIIQNIAI TDINPKYVWG GDAITINNAD MVWIDHVTTA RIGRQHLVLG
210 220 230 240 250
TSASNRVTIS NNYFNGVSSY SATCDGYHYW GIYLDGSNDL VTMKGNYIYH
260 270 280 290 300
FSGRSPKVQG NTLLHAVNNY WYDSSGHAFE IGSGGYVLAE GNVFQNIDTI
310 320 330 340 350
VESPVDGQLF TSPDSTTNKV CSTYLGHVCQ VNGFGSSGTF SQADTGFLSN
360 370
FAGKNIASAS AYTVVQSSVP SSAGQGKI
Length:378
Mass (Da):39,318
Last modified:May 18, 2010 - v2
Checksum:iAFF89A671924F95C
GO

Sequence cautioni

The sequence EAW23742 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027686 Genomic DNA. Translation: EAW23742.1. Different initiation.
RefSeqiXP_001265639.1. XM_001265638.1.

Genome annotation databases

GeneIDi4592857.
KEGGinfi:NFIA_033080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027686 Genomic DNA. Translation: EAW23742.1. Different initiation.
RefSeqiXP_001265639.1. XM_001265638.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4592857.
KEGGinfi:NFIA_033080.

Organism-specific databases

EuPathDBiFungiDB:NFIA_033080.

Phylogenomic databases

HOGENOMiHOG000175137.
KOiK01732.
OMAiSATCNGR.
OrthoDBiEOG092C2XJ9.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPELA_NEOFI
AccessioniPrimary (citable) accession number: A1CYC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: May 18, 2010
Last modified: September 7, 2016
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.