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Protein

Methionine aminopeptidase 2-1

Gene

NFIA_109310

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei197 – 1971SubstrateUniRule annotation
Metal bindingi217 – 2171Divalent metal cation 1UniRule annotation
Metal bindingi228 – 2281Divalent metal cation 1UniRule annotation
Metal bindingi228 – 2281Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi297 – 2971Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
Binding sitei305 – 3051SubstrateUniRule annotation
Metal bindingi330 – 3301Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi425 – 4251Divalent metal cation 1UniRule annotation
Metal bindingi425 – 4251Divalent metal cation 2; catalyticUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2-1UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2-1UniRule annotation
Short name:
MetAP 2-1UniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
ORF Names:NFIA_109310
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
ProteomesiUP000006702 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:NFIA_109310.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Methionine aminopeptidase 2-1PRO_0000407631Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi36630.CADNFIAP00009378.

Structurei

3D structure databases

ProteinModelPortaliA1CXT5.
SMRiA1CXT5. Positions 75-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 7112Poly-LysAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0024.
HOGENOMiHOG000226278.
KOiK01265.
OMAiEMEALCI.
OrthoDBiEOG7BGHW3.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1CXT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQVTEKLQ DLHLNGQNGD AKANSTAVGQ TEAGEAEDDS DDEKEDGNAA
60 70 80 90 100
PEAGAGGAAK KKKRKSKKKK KGGAKVQSSP PRVPVSNLFP NNQYPEGEIV
110 120 130 140 150
EYKNENSYRT TNEEKRYLDR MNNDFLQEYR QAAEVHRQVR QYAQRTIKPG
160 170 180 190 200
QTLTEIAEGI EDAVRALTGH QGLEEGDNLK GGMGFPCGLS INHCAAHYTP
210 220 230 240 250
NAGNKMVLQQ GDVMKVDFGA HINGRIVDSA FTMTFDPVYD PLLEAVKDAT
260 270 280 290 300
NTGIREAGID VRMSDIGAAI QEAMESYEVE LNGTMYPVKC IRNLNGHNID
310 320 330 340 350
QHIIHGGKSV PIVKGSDQTK MEEGETFAIE TFGSTGKGYV REDMETSHYA
360 370 380 390 400
LIPDAPSVPL RLSSAKNLLN VINKNFGTLP FCRRYLDRLG QEKYLLGLNN
410 420 430 440
LVSSGIVQDY PPLCDVKGSY TAQFEHTILL RPTVKEVISR GDDY
Length:444
Mass (Da):48,772
Last modified:January 23, 2007 - v1
Checksum:i2130708762ABA841
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027685 Genomic DNA. Translation: EAW25437.1.
RefSeqiXP_001267334.1. XM_001267333.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00009618; CADNFIAP00009378; CADNFIAG00009618.
GeneIDi4593305.
KEGGinfi:NFIA_109310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027685 Genomic DNA. Translation: EAW25437.1.
RefSeqiXP_001267334.1. XM_001267333.1.

3D structure databases

ProteinModelPortaliA1CXT5.
SMRiA1CXT5. Positions 75-444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi36630.CADNFIAP00009378.

Protein family/group databases

MEROPSiM24.002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADNFIAT00009618; CADNFIAP00009378; CADNFIAG00009618.
GeneIDi4593305.
KEGGinfi:NFIA_109310.

Organism-specific databases

EuPathDBiFungiDB:NFIA_109310.

Phylogenomic databases

eggNOGiCOG0024.
HOGENOMiHOG000226278.
KOiK01265.
OMAiEMEALCI.
OrthoDBiEOG7BGHW3.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Entry informationi

Entry nameiMAP21_NEOFI
AccessioniPrimary (citable) accession number: A1CXT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.