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A1CWA9 (TRMB_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trm8
ORF Names:NFIA_103990
OrganismNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]
Taxonomic identifier331117 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with trm82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370599

Regions

Region122 – 1232S-adenosyl-L-methionine binding By similarity
Region209 – 2102S-adenosyl-L-methionine binding By similarity
Region332 – 3343S-adenosyl-L-methionine binding By similarity

Sites

Active site2321 By similarity
Binding site991S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site2291S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A1CWA9 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 36CA4ABBD9EEDB41

FASTA36040,001
        10         20         30         40         50         60 
MTPPPPKRQK RDEYRKATAE AASQPGPSDV AEIKLPKKKY YRQRAHANPF SDHHLNYPLS 

        70         80         90        100        110        120 
PAHMDWSSHY PAFVDPDPSH TNLAGARKLL KDVEVVDIGC GFGGLLIGLA PLLPESLIVG 

       130        140        150        160        170        180 
MEIRVSVLEY VTTRIQALRA QQLKLRAATA AATAASETPS QQQAQIDGKQ ANANAAADAA 

       190        200        210        220        230        240 
SPVLSTDTEH TPTTLVPGSY ENISAIRSNT MKFFPNFFAR HQLSKIFICF PDPHFKARKH 

       250        260        270        280        290        300 
KARIISETLN AEYAYALRPG GLLYTITDVE EYHHWILRHF GVESGAEEES EEKSTSANAN 

       310        320        330        340        350        360 
ANANAGVREL FERVSEEELE KDECVRVMKE ATEEGKKVAR NKGNKYVAVF RRKTDPEWPA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS027685 Genomic DNA. Translation: EAW24911.1.
RefSeqXP_001266808.1. XM_001266807.1.

3D structure databases

ProteinModelPortalA1CWA9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING36630.CADNFIAP00009899.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADNFIAT00010139; CADNFIAP00009899; CADNFIAG00010139.
GeneID4593789.
KEGGnfi:NFIA_103990.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000260965.
KOK03439.
OMARAHSNPI.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 3 hits.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 2 hits.
[Graphical view]
SUPFAMSSF53335. SSF53335. 2 hits.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_NEOFI
AccessionPrimary (citable) accession number: A1CWA9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways