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A1CWA9

- TRMB_NEOFI

UniProt

A1CWA9 - TRMB_NEOFI

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei229 – 2291S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei232 – 2321UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:NFIA_103990
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
ProteomesiUP000006702: Unassembled WGS sequence

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360tRNA (guanine-N(7)-)-methyltransferasePRO_0000370599Add
BLAST

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Protein-protein interaction databases

STRINGi36630.CADNFIAP00009899.

Structurei

3D structure databases

ProteinModelPortaliA1CWA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 1232S-adenosyl-L-methionine bindingUniRule annotation
Regioni209 – 2102S-adenosyl-L-methionine bindingUniRule annotation
Regioni332 – 3343S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
KOiK03439.
OMAiRAHSNPI.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 2 hits.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1CWA9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTPPPPKRQK RDEYRKATAE AASQPGPSDV AEIKLPKKKY YRQRAHANPF
60 70 80 90 100
SDHHLNYPLS PAHMDWSSHY PAFVDPDPSH TNLAGARKLL KDVEVVDIGC
110 120 130 140 150
GFGGLLIGLA PLLPESLIVG MEIRVSVLEY VTTRIQALRA QQLKLRAATA
160 170 180 190 200
AATAASETPS QQQAQIDGKQ ANANAAADAA SPVLSTDTEH TPTTLVPGSY
210 220 230 240 250
ENISAIRSNT MKFFPNFFAR HQLSKIFICF PDPHFKARKH KARIISETLN
260 270 280 290 300
AEYAYALRPG GLLYTITDVE EYHHWILRHF GVESGAEEES EEKSTSANAN
310 320 330 340 350
ANANAGVREL FERVSEEELE KDECVRVMKE ATEEGKKVAR NKGNKYVAVF
360
RRKTDPEWPA
Length:360
Mass (Da):40,001
Last modified:January 23, 2007 - v1
Checksum:i36CA4ABBD9EEDB41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027685 Genomic DNA. Translation: EAW24911.1.
RefSeqiXP_001266808.1. XM_001266807.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00010139; CADNFIAP00009899; CADNFIAG00010139.
GeneIDi4593789.
KEGGinfi:NFIA_103990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027685 Genomic DNA. Translation: EAW24911.1 .
RefSeqi XP_001266808.1. XM_001266807.1.

3D structure databases

ProteinModelPortali A1CWA9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 36630.CADNFIAP00009899.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADNFIAT00010139 ; CADNFIAP00009899 ; CADNFIAG00010139 .
GeneIDi 4593789.
KEGGi nfi:NFIA_103990.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000260965.
KOi K03439.
OMAi RAHSNPI.
OrthoDBi EOG708W9T.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 3 hits.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 2 hits.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Entry informationi

Entry nameiTRMB_NEOFI
AccessioniPrimary (citable) accession number: A1CWA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3