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A1CWA9

- TRMB_NEOFI

UniProt

A1CWA9 - TRMB_NEOFI

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei99 – 991S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei229 – 2291S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei232 – 2321UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    Transfer RNA methyltransferase 8UniRule annotation
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trm8
    ORF Names:NFIA_103990
    OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
    Taxonomic identifieri331117 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
    ProteomesiUP000006702: Unassembled WGS sequence

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360tRNA (guanine-N(7)-)-methyltransferasePRO_0000370599Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with trm82.UniRule annotation

    Protein-protein interaction databases

    STRINGi36630.CADNFIAP00009899.

    Structurei

    3D structure databases

    ProteinModelPortaliA1CWA9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni122 – 1232S-adenosyl-L-methionine bindingUniRule annotation
    Regioni209 – 2102S-adenosyl-L-methionine bindingUniRule annotation
    Regioni332 – 3343S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000260965.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG708W9T.

    Family and domain databases

    Gene3Di3.40.50.150. 3 hits.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 2 hits.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1CWA9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPPPPKRQK RDEYRKATAE AASQPGPSDV AEIKLPKKKY YRQRAHANPF    50
    SDHHLNYPLS PAHMDWSSHY PAFVDPDPSH TNLAGARKLL KDVEVVDIGC 100
    GFGGLLIGLA PLLPESLIVG MEIRVSVLEY VTTRIQALRA QQLKLRAATA 150
    AATAASETPS QQQAQIDGKQ ANANAAADAA SPVLSTDTEH TPTTLVPGSY 200
    ENISAIRSNT MKFFPNFFAR HQLSKIFICF PDPHFKARKH KARIISETLN 250
    AEYAYALRPG GLLYTITDVE EYHHWILRHF GVESGAEEES EEKSTSANAN 300
    ANANAGVREL FERVSEEELE KDECVRVMKE ATEEGKKVAR NKGNKYVAVF 350
    RRKTDPEWPA 360
    Length:360
    Mass (Da):40,001
    Last modified:January 23, 2007 - v1
    Checksum:i36CA4ABBD9EEDB41
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027685 Genomic DNA. Translation: EAW24911.1.
    RefSeqiXP_001266808.1. XM_001266807.1.

    Genome annotation databases

    EnsemblFungiiCADNFIAT00010139; CADNFIAP00009899; CADNFIAG00010139.
    GeneIDi4593789.
    KEGGinfi:NFIA_103990.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027685 Genomic DNA. Translation: EAW24911.1 .
    RefSeqi XP_001266808.1. XM_001266807.1.

    3D structure databases

    ProteinModelPortali A1CWA9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 36630.CADNFIAP00009899.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADNFIAT00010139 ; CADNFIAP00009899 ; CADNFIAG00010139 .
    GeneIDi 4593789.
    KEGGi nfi:NFIA_103990.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000260965.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG708W9T.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 3 hits.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

    Entry informationi

    Entry nameiTRMB_NEOFI
    AccessioniPrimary (citable) accession number: A1CWA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3