ID   PGLRA_NEOFI             Reviewed;         368 AA.
AC   A1CVV8;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   03-APR-2013, entry version 37.
DE   RecName: Full=Probable endopolygalacturonase A;
DE            EC=3.2.1.15;
DE   AltName: Full=Pectinase A;
DE   AltName: Full=Polygalacturonase A;
DE   Flags: Precursor;
GN   Name=pgaA; Synonyms=pecA; ORFNames=NFIA_102450;
OS   Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL
OS   181) (Aspergillus fischerianus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae; Neosartorya.
OX   NCBI_TaxID=331117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P.,
RA   Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A.,
RA   Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A.,
RA   Galens K., Fraser-Liggett C.M., Haas B.J., Inman J.M., Kent R.,
RA   Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M.,
RA   Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R.,
RA   Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R.,
RA   Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Involved in maceration and soft-rotting of plant tissue.
CC       Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate
CC       in the smooth region of the plant cell wall (By similarity).
CC   -!- CATALYTIC ACTIVITY: Random hydrolysis of (1->4)-alpha-D-
CC       galactosiduronic linkages in pectate and other galacturonans.
CC   -!- SUBCELLULAR LOCATION: Secreted (By similarity).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family.
CC   -!- SIMILARITY: Contains 7 PbH1 repeats.
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DR   EMBL; DS027685; EAW24760.1; -; Genomic_DNA.
DR   RefSeq; XP_001266657.1; XM_001266656.1.
DR   ProteinModelPortal; A1CVV8; -.
DR   SMR; A1CVV8; 33-368.
DR   STRING; 36630.CADNFIAP00009335; -.
DR   EnsemblFungi; CADNFIAT00009575; CADNFIAP00009335; CADNFIAG00009575.
DR   GeneID; 4594124; -.
DR   KEGG; nfi:NFIA_102450; -.
DR   eggNOG; COG5434; -.
DR   HOGENOM; HOG000193107; -.
DR   KO; K01184; -.
DR   OrthoDB; EOG46DQBJ; -.
DR   GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR   GO; GO:0004650; F:polygalacturonase activity; ISS:UniProtKB.
DR   GO; GO:0045490; P:pectin catabolic process; ISS:UniProtKB.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR000743; Glyco_hydro_28.
DR   InterPro; IPR006626; PbH1.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF00295; Glyco_hydro_28; 1.
DR   SMART; SM00710; PbH1; 7.
DR   SUPFAM; SSF51126; Pectin_lyas_like; 1.
DR   PROSITE; PS00502; POLYGALACTURONASE; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Complete proteome; Disulfide bond;
KW   Glycoprotein; Glycosidase; Hydrolase; Repeat; Secreted; Signal;
KW   Zymogen.
FT   SIGNAL        1     18       Potential.
FT   PROPEP       19     31       Potential.
FT                                /FTId=PRO_0000393642.
FT   CHAIN        32    368       Probable endopolygalacturonase A.
FT                                /FTId=PRO_0000393643.
FT   REPEAT      140    162       PbH1 1.
FT   REPEAT      167    192       PbH1 2.
FT   REPEAT      193    214       PbH1 3.
FT   REPEAT      215    235       PbH1 4.
FT   REPEAT      244    265       PbH1 5.
FT   REPEAT      273    295       PbH1 6.
FT   REPEAT      307    352       PbH1 7.
FT   ACT_SITE    207    207       Proton donor (By similarity).
FT   ACT_SITE    229    229       By similarity.
FT   CARBOHYD    246    246       N-linked (GlcNAc...) (Potential).
FT   DISULFID     35     50       By similarity.
FT   DISULFID    209    225       By similarity.
FT   DISULFID    335    340       By similarity.
FT   DISULFID    359    368       By similarity.
SQ   SEQUENCE   368 AA;  38109 MW;  8D42D1E8C43D38BA CRC64;
     MRSVELLSLA ALGSLVAAAP APSRVSDLTK RSSTCTFTAA SQATESASGC SEIVLDNIEV
     PAGETLDLSD VDDGTTIVFE GTTTFGYKEW SGPLIRFGGK DITVKQNSGA VIDGEGSRWW
     DGEGTNGGKT KPKFMYAHSL EDSTITGLSI KNTPVQAISV QATNLYLIDI TIDNSDGDDN
     GGHNTDGFDI SESTGVYIRG ATVKNQDDCI AINSGENIEF SGGTCSGGHG LSIGSVGGRD
     DNTVKNVTIT DSTVTDSANG VRIKTVYDAT GSVSEVTYSN IKLSGITDYG IVIEQDYENG
     SPTGTPTTGV PITDLTIDGV TGTVESDAVE VYILCGDGSC SDWTWEGVDI TGGETSSKCE
     NVPSGASC
//