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Protein

Protostadienol synthase A

Gene

pdsA

Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Protostadienol synthase which cyclizes (3S)-oxidosqualene to (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the biosynthetic precursor of helvolic acid, a secundary metabolite which promotes virulence.By similarity

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3-beta-ol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei240 – 2401Proton acceptorBy similarity
Active sitei463 – 4631Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Protostadienol synthase A (EC:5.4.99.32)
Gene namesi
Name:pdsA
ORF Names:NFIA_101360
OrganismiNeosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus)
Taxonomic identifieri331117 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeNeosartorya
ProteomesiUP000006702 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:NFIA_101360.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 738738Protostadienol synthase APRO_0000415497Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA1CVK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati132 – 17342PFTB 1Add
BLAST
Repeati490 – 53142PFTB 2Add
BLAST
Repeati567 – 60741PFTB 3Add
BLAST
Repeati616 – 66348PFTB 4Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 4 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000234317.
KOiK15811.
OMAiITPLIMQ.
OrthoDBiEOG70610B.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.

Sequencei

Sequence statusi: Complete.

A1CVK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTDSSMPAT VIGKAEFSNT KAASEFGTNL SRWRLNVDNG RHMWEYLESE
60 70 80 90 100
DEARKRPQSI LEKYWLGLPY ELPARPRATR ALEAVENGWE FFKRLQTADG
110 120 130 140 150
HWGCNDDGPL FVTSGMVIAR YIVGIPIDSH MKQEMCRYLL NVVNEDGGWG
160 170 180 190 200
LFIQSPSTVF GTVMNYCMLR ILGLGPEHPA MARARNTLHR LGSASATPTW
210 220 230 240 250
GKFWLCVLGV YEWEGMIPLP PEPLLVPASL PFNPGKWWVH TRNVYISMSY
260 270 280 290 300
LYGHRFSMPP NKLVQALRDE LYDIPYEQIN WPAQRTNVSA ADRLTDPTWI
310 320 330 340 350
QRSFTSALTT YETFKIPFLR RRALNEALFQ IETETRNTHY LCIAPVSFAS
360 370 380 390 400
NMLALYHAHG RDSHWIRGMR DRFIDPMWLC REGLAASGTN GTSLWDTALT
410 420 430 440 450
VQATIDAGLA ARPENQAILR KALEFIDNSQ IREDPLGVHH VYRQPTRGAW
460 470 480 490 500
PFSTRDQSYA VSDTTAEAVK VVVLLQQIEG FPSRISDERL QQAIDLILGM
510 520 530 540 550
ENAGGGFSAY EPVRGPKFLE LLNITELYEN VMTDNLYPEC TSSVIMSLTT
560 570 580 590 600
FAREYPTYRA RDIQACVSRS VDYLLRSQYP NGGWFASWGV CFTYATMFAL
610 620 630 640 650
QGLACMGRNE SNCAACQRAC SFLLQHQNPD GGWGESLDTV RFKQYLPHPD
660 670 680 690 700
GSQVTNTAYA VIGLLAARCG NHEAIRRGVA YLMKEQQDTG EWLPGALEGV
710 720 730
FAPPGGMRYP NYKFHFTLMA LGRYAAVHGD ECLAAQLV
Length:738
Mass (Da):83,217
Last modified:January 23, 2007 - v1
Checksum:iC36579825E6BA4AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027685 Genomic DNA. Translation: EAW24652.1.
RefSeqiXP_001266549.1. XM_001266548.1.

Genome annotation databases

EnsemblFungiiCADNFIAT00009997; CADNFIAP00009757; CADNFIAG00009997.
GeneIDi4594041.
KEGGinfi:NFIA_101360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027685 Genomic DNA. Translation: EAW24652.1.
RefSeqiXP_001266549.1. XM_001266548.1.

3D structure databases

ProteinModelPortaliA1CVK0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADNFIAT00009997; CADNFIAP00009757; CADNFIAG00009997.
GeneIDi4594041.
KEGGinfi:NFIA_101360.

Organism-specific databases

EuPathDBiFungiDB:NFIA_101360.

Phylogenomic databases

HOGENOMiHOG000234317.
KOiK15811.
OMAiITPLIMQ.
OrthoDBiEOG70610B.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Entry informationi

Entry nameiPDSA_NEOFI
AccessioniPrimary (citable) accession number: A1CVK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.