A1CVK0 (PDSA_NEOFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protostadienol synthase A
EC=5.4.99.32

Gene names

Name:	pdsA
ORF Names:	NFIA_101360

Organism

Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) (Aspergillus fischerianus) [Complete proteome]

Taxonomic identifier

331117 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Neosartorya › Neosartorya fischeri group › Neosartorya fischeri

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protostadienol synthase which cyclizes (3S)-oxidosqualene to (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the biosynthetic precursor of helvolic acid, a secundary metabolite which promotes virulence By similarity.
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3-beta-ol.
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 4 PFTB repeats.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Virulence
Domain	Repeat
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	intramolecular transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	Protostadienol synthase A		PRO_0000415497
Regions
	Repeat	132 – 173	42	PFTB 1
	Repeat	490 – 531	42	PFTB 2
	Repeat	567 – 607	41	PFTB 3
	Repeat	616 – 663	48	PFTB 4
Sites
	Active site	240	1	Proton acceptor By similarity
	Active site	463	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1CVK0 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: C36579825E6BA4AC	FASTA	738	83,217
	10 20 30 40 50 60 MTTDSSMPAT VIGKAEFSNT KAASEFGTNL SRWRLNVDNG RHMWEYLESE DEARKRPQSI 70 80 90 100 110 120 LEKYWLGLPY ELPARPRATR ALEAVENGWE FFKRLQTADG HWGCNDDGPL FVTSGMVIAR 130 140 150 160 170 180 YIVGIPIDSH MKQEMCRYLL NVVNEDGGWG LFIQSPSTVF GTVMNYCMLR ILGLGPEHPA 190 200 210 220 230 240 MARARNTLHR LGSASATPTW GKFWLCVLGV YEWEGMIPLP PEPLLVPASL PFNPGKWWVH 250 260 270 280 290 300 TRNVYISMSY LYGHRFSMPP NKLVQALRDE LYDIPYEQIN WPAQRTNVSA ADRLTDPTWI 310 320 330 340 350 360 QRSFTSALTT YETFKIPFLR RRALNEALFQ IETETRNTHY LCIAPVSFAS NMLALYHAHG 370 380 390 400 410 420 RDSHWIRGMR DRFIDPMWLC REGLAASGTN GTSLWDTALT VQATIDAGLA ARPENQAILR 430 440 450 460 470 480 KALEFIDNSQ IREDPLGVHH VYRQPTRGAW PFSTRDQSYA VSDTTAEAVK VVVLLQQIEG 490 500 510 520 530 540 FPSRISDERL QQAIDLILGM ENAGGGFSAY EPVRGPKFLE LLNITELYEN VMTDNLYPEC 550 560 570 580 590 600 TSSVIMSLTT FAREYPTYRA RDIQACVSRS VDYLLRSQYP NGGWFASWGV CFTYATMFAL 610 620 630 640 650 660 QGLACMGRNE SNCAACQRAC SFLLQHQNPD GGWGESLDTV RFKQYLPHPD GSQVTNTAYA 670 680 690 700 710 720 VIGLLAARCG NHEAIRRGVA YLMKEQQDTG EWLPGALEGV FAPPGGMRYP NYKFHFTLMA 730 LGRYAAVHGD ECLAAQLV

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027685 Genomic DNA. Translation: EAW24652.1.
RefSeq	XP_001266549.1. XM_001266548.1.
3D structure databases
ProteinModelPortal	A1CVK0.
ModBase	Search...
Protein-protein interaction databases
STRING	36630.CADNFIAP00009757.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADNFIAT00009997; CADNFIAP00009757; CADNFIAG00009997.
GeneID	4594041.
KEGG	nfi:NFIA_101360.
Phylogenomic databases
HOGENOM	HOG000234317.
KO	K15811.
OrthoDB	EOG4F7RW1.
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDSA_NEOFI

Accession

Primary (citable) accession number: A1CVK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2012
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program