Reviewed, UniProtKB/Swiss-Prot A1CRK9 (MCR1_ASPCL)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase

Gene names

Name:	mcr1
ORF Names:	ACLA_030130

Organism

Aspergillus clavatus [Complete proteome]

Taxonomic identifier

5057 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	NADH-cytochrome b5 reductase 2		PRO_0000330170
Regions
	Transmembrane	32 – 48	17	Potential
	Domain	72 – 176	105	FAD-binding FR-type
	Nucleotide binding	179 – 214	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1CRK9-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 78259A75EFB29258	FASTA	322	36,040
	10 20 30 40 50 60 MFARQPLRFA QPLKQGFRKY STEAPAKGKS SLAPIYISVG LAGLGVGLYR YSTASAETPV 70 80 90 100 110 120 VDRPKVFTGG EQGWVDLKLS EIENLSHNTK RLRFEFADKE AVSGLQVASA LLTKFKPAEG 130 140 150 160 170 180 KPVIRPYTPV SDEDQPGYLD LVVKVYPNGP MSEHLHSMNV DQRLEFKGPI PKYPWETNKH 190 200 210 220 230 240 KHICLIAGGT GITPMYQLAR QIFKNPEDQT KVTLVFGNVS EEDILLKKEL QELENTHPRR 250 260 270 280 290 300 FKAFYVLDNP PKEWTGGKGY VTKELLKTVL PEPKEEDIKI FVCGPPGMYK AISGPKVSPK 310 320 DQGELTGLLA ELGYNKDQVY KF

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed: 18404212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS027059 Genomic DNA. Translation: EAW08280.1.
RefSeq	XP_001269706.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4701749.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_ASPCL

Accession

Primary (citable) accession number: A1CRK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)