Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A1CP08 (MNS1B_ASPCL) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

EC=3.2.1.113
Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B
Gene names
Name:mns1B
Synonyms:msdS
ORF Names:ACLA_020870
OrganismAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]
Taxonomic identifier344612 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 By similarity.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactor

Ca2+. Can also use Mg2+, but with lower efficiency By similarity.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasmic vesicle lumen By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasmic vesicle
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotein glycosylation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasmic membrane-bounded vesicle lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

mannosyl-oligosaccharide 1,2-alpha-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 511493Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
PRO_0000394816

Sites

Active site3701Proton donor By similarity

Amino acid modifications

Glycosylation901N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential
Disulfide bond327 ↔ 356 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1CP08 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: F13A052EC4FDD714

FASTA51156,325
        10         20         30         40         50         60 
MHLPSLSLAW ALAGSSLALP QAEQPVLQDD RASSRAAAVK EAFSHAWDGY KKYAFPHDEL 

        70         80         90        100        110        120 
HPISNGYGNS RNGWGASAVD ALSTAIVMRN ATIVHEILDH IATIDYSKTS DTVSLFETTI 

       130        140        150        160        170        180 
RYLGGMLSGY DLLKGPAADL VKDTSKVDIL LKQSKNLGDI LKFAFDTPSG VPYNNINITS 

       190        200        210        220        230        240 
HGHDGAKTNG LAVTGTLVLE WTRLSDLTGD PEYAQLSQKA ESYLLHPQPS SAEPFPGLIG 

       250        260        270        280        290        300 
SNIDIKTGQF TDGHVSWNGG DDSYYEYLIK MYVYDPTRFA LYRDRWVAAA ESSIEHLASH 

       310        320        330        340        350        360 
PASRPDLTFL ATYDGGRYGL SSQHLACFDG GNFLLGGTVL DRRDLIDFGL ALVDACHETY 

       370        380        390        400        410        420 
SQTLTGIGPE SFGWDANSVP ADQKELYERA GFYVQNGAYI LRPEVIESFY YAWRVTGRSE 

       430        440        450        460        470        480 
YRDWVWTAFV AINKTCRTGS GYAGLTNVNA ENGGGRYDNQ ESFLFAEVLK YAYLTHSEEL 

       490        500        510 
MAAEDAWQVQ RGDGNQFVFN TEAHPIRVHH S 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS027059 Genomic DNA. Translation: EAW07379.1.
RefSeqXP_001268805.1. XM_001268804.1.

3D structure databases

ProteinModelPortalA1CP08.
SMRA1CP08. Positions 34-508.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5057.CADACLAP00002234.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADACLAT00002281; CADACLAP00002234; CADACLAG00002281.
GeneID4701674.
KEGGact:ACLA_020870.

Phylogenomic databases

eggNOGNOG300315.
HOGENOMHOG000181987.
KOK01230.
OMAGPESFGW.
OrthoDBEOG7BP8BH.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

Gene3D1.50.10.50. 1 hit.
InterProIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERPTHR11742. PTHR11742. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. SSF48225. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNS1B_ASPCL
AccessionPrimary (citable) accession number: A1CP08
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: January 23, 2007
Last modified: November 13, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries