A1CLD2 (BUD32_ASPCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase bud32
EC=2.7.11.1

Gene names

Name:	bud32
ORF Names:	ACLA_041780

Organism

Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]

Taxonomic identifier

344612 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation By similarity. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Component of the EKC/KEOPS complex By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Chromosome › telomere By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosome Cytoplasm Nucleus Telomere
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Serine/threonine-protein kinase bud32		PRO_0000278905
Regions
	Domain	20 – 277	258	Protein kinase
	Nucleotide binding	26 – 34	9	ATP By similarity
Sites
	Active site	172	1	Proton acceptor By similarity
	Binding site	49	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1CLD2 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: C334D54533C62B09	FASTA	277	31,061
	10 20 30 40 50 60 MSSAEPYTPP PLPSPFTNTT PPPQLLTQGA EAHLYKTVFL SPSTPAALKV RPSKPYRHPI 70 80 90 100 110 120 LDRRLTRQRI LQEARCLVKL VREGVNVPAV LALDWEGQNT EKGFGGAWLM MEWVEGLVVR 130 140 150 160 170 180 VVLERWERWM KRNQGSSGAE ELKEEENWVR DLLRRIGRAV GALHKAGVIH GDLTTSNLIL 190 200 210 220 230 240 RPPGHVGEQA DTGESSPSME GDVVLIDFGL ASQSLQDEDR AVDLYVLERA FGSTHPRTEP 250 260 270 FFEEVLNGYR ESYKGASSAL KRLEDVRMRG RKRSMIG

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed: 18404212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027056 Genomic DNA. Translation: EAW09956.1.
RefSeq	XP_001271382.1. XM_001271381.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1CLD2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADACLAT00003372; CADACLAP00003292; CADACLAG00003372.
GeneID	4703225.
KEGG	act:ACLA_041780.
Phylogenomic databases
GeneTree	EFGT00050000005785.
OrthoDB	EOG4R7ZKZ.
Family and domain databases
InterPro	IPR022495. Kinase-assoc_endopept-1. IPR011009. Kinase-like_dom. IPR010440. LipoPS_kinase. IPR000719. Prot_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
KO	K08851.
Pfam	PF06293. Kdo. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR03724. Arch_bud32. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUD32_ASPCL

Accession

Primary (citable) accession number: A1CLD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program