A1CIW4 (A1CIW4_ASPCL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Alpha amylase, putative EMBL EAW10819.1

Gene names

ORF Names:

ACLA_052920 EMBL EAW10819.1

Organism

Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]

Taxonomic identifier

344612 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus clavatus

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Calcium PIRSR PIRSR001024-3 Metal-binding PIRSR PIRSR001024-3
PTM	Disulfide bond PIRSR PIRSR001024-4
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha-amylase activity Inferred from electronic annotation. Source: InterPro calcium ion binding Inferred from electronic annotation. Source: InterPro starch binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	225	1	Nucleophile By similarity PIRSR PIRSR001024-1
	Active site	249	1	Proton donor By similarity PIRSR PIRSR001024-1
	Metal binding	140	1	Calcium 1 By similarity PIRSR PIRSR001024-3
	Metal binding	181	1	Calcium 1; via carbonyl oxygen By similarity PIRSR PIRSR001024-3
	Metal binding	194	1	Calcium 1 By similarity PIRSR PIRSR001024-3
	Metal binding	225	1	Calcium 2 By similarity PIRSR PIRSR001024-3
	Metal binding	229	1	Calcium 1; via carbonyl oxygen By similarity PIRSR PIRSR001024-3
	Metal binding	249	1	Calcium 2 By similarity PIRSR PIRSR001024-3
	Site	316	1	Transition state stabilizer By similarity PIRSR PIRSR001024-2
Amino acid modifications
	Disulfide bond	49 ↔ 57		By similarity PIRSR PIRSR001024-4
	Disulfide bond	169 ↔ 183		By similarity PIRSR PIRSR001024-4
	Disulfide bond	259 ↔ 302		By similarity PIRSR PIRSR001024-4
	Disulfide bond	459 ↔ 494		By similarity PIRSR PIRSR001024-4

Sequences

	Sequence		Length	Mass (Da)
	A1CIW4 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: D26D7D66BA69675F	FASTA	636	68,945
	10 20 30 40 50 60 MKLLALTTAF ALLGKGVFGL TPAEWRGQSI YFLITDRFAR TDGSTTAPCD LSQRAYCGGS 70 80 90 100 110 120 WQGIIKQLDY IQGMGFTAIW ITPITEQIPQ DTAEGSAFHG YWQKDIYNVN SHFGTADDIR 130 140 150 160 170 180 ALSKALHDRG MYLMIDVVAN HMGYNGPGAS TDFSTFTPFN SASYFHSYCP INNYNDQSQV 190 200 210 220 230 240 ENCWLGDNTV ALADLYTQHS DVRNIWYSWI KEIVGNYSAD GLRIDTVKHV EKDFWTGYTQ 250 260 270 280 290 300 AAGVYTVGEV LDGDPAYTCP YQGYVDGVLN YPIYYPLLRA FESSSGSMGD LYNMINSVAS 310 320 330 340 350 360 DCKDPTVLGS FIENHDNPRF ASYTKDMSQA KAVISYVILS DGIPIIYSGQ EQHYSGGNDP 370 380 390 400 410 420 YNREAIWLSG YSTTSELYKF IATTNKIRQL AISKDSSYLT SRNNPFYTDS NTIAMRKGSG 430 440 450 460 470 480 GSQVITVLSN SGSNGGSYTL NLGNSGYSSG ANLVEVYTCS SVTVGSDGKI PVPMASGLPR 490 500 510 520 530 540 VLVPASWMSG SGLCGSSSTT TLVTATTTPT GSSSSTTLAT AVTTPTGSCK TATTVPVVLE 550 560 570 580 590 600 ESVRTSYGEN IFISGSIPQL GSWNPDKAVA LSSSQYTSSN PLWAVTLDLP VGTSFEYKFL 610 620 630 KKEQNGGVAW ENDPNRSYTV PEACAGTSQK VDSSWR

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027054 Genomic DNA. Translation: EAW10819.1.
RefSeq	XP_001272245.1. XM_001272244.1.
3D structure databases
ProteinModelPortal	A1CIW4.
SMR	A1CIW4. Positions 21-494.
ModBase	Search...
Protein-protein interaction databases
STRING	5057.CADACLAP00005009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADACLAT00005110; CADACLAP00005009; CADACLAG00005110.
GeneID	4704010.
KEGG	act:ACLA_052920.
Phylogenomic databases
HOGENOM	HOG000165530.
KO	K01176.
OrthoDB	EOG4SFDF6.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR013777. A-amylase_fun. IPR015340. A_amylase_DUF1966_C. IPR013784. Carb-bd-like_fold. IPR002044. CBM_fam20. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF00686. CBM_20. 1 hit. PF09260. DUF1966. 1 hit. [Graphical view]
PIRSF	PIRSF001024. Alph-amyl_fung. 1 hit.
SMART	SM00642. Aamy. 1 hit. SM01065. CBM_2. 1 hit. [Graphical view]
SUPFAM	SSF49452. CBD_4. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS51166. CBM20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1CIW4_ASPCL

Accession

Primary (citable) accession number: A1CIW4

Entry history

Integrated into UniProtKB/TrEMBL:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)