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A1CIF1

- TRMB_ASPCL

UniProt

A1CIF1 - TRMB_ASPCL

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei94 – 941S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei222 – 2221S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei225 – 2251UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    Transfer RNA methyltransferase 8UniRule annotation
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trm8
    ORF Names:ACLA_051280
    OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
    Taxonomic identifieri344612 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006701: Unassembled WGS sequence

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339tRNA (guanine-N(7)-)-methyltransferasePRO_0000370584Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with trm82.UniRule annotation

    Protein-protein interaction databases

    STRINGi5057.CADACLAP00004661.

    Structurei

    3D structure databases

    ProteinModelPortaliA1CIF1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni117 – 1182S-adenosyl-L-methionine bindingUniRule annotation
    Regioni202 – 2032S-adenosyl-L-methionine bindingUniRule annotation
    Regioni311 – 3133S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000260965.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG708W9T.

    Family and domain databases

    Gene3Di3.40.50.150. 3 hits.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 2 hits.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1CIF1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPPPAKRQK RNEYRKANTA VADDASEIRL PQKKFYRQRA HANPFSDHQL    50
    NYPLSPAHMD WSSHYPAFVD PEPSHTNLAG TRKLLKDVEV VDIGCGFGGL 100
    LIGLAPLLPE SLIVGMEIRV SVLEYVTARI QALRSQQQKL RSSAIPSESS 150
    PAAQQPQQHH QQQLQATETA ADAASPSSPD ATGETLSLVP GNYQNISAIR 200
    SNTMKFFPNF FARHQLSKIF ICFPDPHFKA RKHKARIISE TLNAEYAYAL 250
    RPGGLLYTIT DVEEYHHWVL RHFGEEGADA GQNAGVTELF ERVSDEELEK 300
    DDCVRVMKEA TEEGKKVTRN KGNKYVAVFR RKADPEWPA 339
    Length:339
    Mass (Da):38,057
    Last modified:January 23, 2007 - v1
    Checksum:iA2EE49C243B87F63
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027054 Genomic DNA. Translation: EAW10656.1.
    RefSeqiXP_001272082.1. XM_001272081.1.

    Genome annotation databases

    EnsemblFungiiCADACLAT00004762; CADACLAP00004661; CADACLAG00004762.
    GeneIDi4703719.
    KEGGiact:ACLA_051280.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027054 Genomic DNA. Translation: EAW10656.1 .
    RefSeqi XP_001272082.1. XM_001272081.1.

    3D structure databases

    ProteinModelPortali A1CIF1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5057.CADACLAP00004661.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADACLAT00004762 ; CADACLAP00004661 ; CADACLAG00004762 .
    GeneIDi 4703719.
    KEGGi act:ACLA_051280.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000260965.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG708W9T.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 3 hits.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

    Entry informationi

    Entry nameiTRMB_ASPCL
    AccessioniPrimary (citable) accession number: A1CIF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3