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A1CIF1

- TRMB_ASPCL

UniProt

A1CIF1 - TRMB_ASPCL

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trm8, ACLA_051280
Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei94 – 941S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei222 – 2221S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei225 – 2251 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    Transfer RNA methyltransferase 8
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trm8
    ORF Names:ACLA_051280
    OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
    Taxonomic identifieri344612 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006701: Unassembled WGS sequence

    Subcellular locationi

    Nucleus By similarity UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000370584Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a complex with trm82 By similarity.UniRule annotation

    Protein-protein interaction databases

    STRINGi5057.CADACLAP00004661.

    Structurei

    3D structure databases

    ProteinModelPortaliA1CIF1.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni117 – 1182S-adenosyl-L-methionine binding By similarity
    Regioni202 – 2032S-adenosyl-L-methionine binding By similarity
    Regioni311 – 3133S-adenosyl-L-methionine binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000260965.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG708W9T.

    Family and domain databases

    Gene3Di3.40.50.150. 3 hits.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 2 hits.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1CIF1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPPPAKRQK RNEYRKANTA VADDASEIRL PQKKFYRQRA HANPFSDHQL    50
    NYPLSPAHMD WSSHYPAFVD PEPSHTNLAG TRKLLKDVEV VDIGCGFGGL 100
    LIGLAPLLPE SLIVGMEIRV SVLEYVTARI QALRSQQQKL RSSAIPSESS 150
    PAAQQPQQHH QQQLQATETA ADAASPSSPD ATGETLSLVP GNYQNISAIR 200
    SNTMKFFPNF FARHQLSKIF ICFPDPHFKA RKHKARIISE TLNAEYAYAL 250
    RPGGLLYTIT DVEEYHHWVL RHFGEEGADA GQNAGVTELF ERVSDEELEK 300
    DDCVRVMKEA TEEGKKVTRN KGNKYVAVFR RKADPEWPA 339
    Length:339
    Mass (Da):38,057
    Last modified:January 23, 2007 - v1
    Checksum:iA2EE49C243B87F63
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027054 Genomic DNA. Translation: EAW10656.1.
    RefSeqiXP_001272082.1. XM_001272081.1.

    Genome annotation databases

    EnsemblFungiiCADACLAT00004762; CADACLAP00004661; CADACLAG00004762.
    GeneIDi4703719.
    KEGGiact:ACLA_051280.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027054 Genomic DNA. Translation: EAW10656.1 .
    RefSeqi XP_001272082.1. XM_001272081.1.

    3D structure databases

    ProteinModelPortali A1CIF1.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 5057.CADACLAP00004661.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADACLAT00004762 ; CADACLAP00004661 ; CADACLAG00004762 .
    GeneIDi 4703719.
    KEGGi act:ACLA_051280.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000260965.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG708W9T.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 3 hits.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

    Entry informationi

    Entry nameiTRMB_ASPCL
    AccessioniPrimary (citable) accession number: A1CIF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: January 23, 2007
    Last modified: June 11, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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