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Protein

Methionine aminopeptidase 2-2

Gene

ACLA_046220

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei197SubstrateUniRule annotation1
Metal bindingi217Divalent metal cation 1UniRule annotation1
Metal bindingi228Divalent metal cation 1UniRule annotation1
Metal bindingi228Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi297Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei305SubstrateUniRule annotation1
Metal bindingi330Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi425Divalent metal cation 1UniRule annotation1
Metal bindingi425Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2-2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2-2UniRule annotation
Short name:
MetAP 2-2UniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
ORF Names:ACLA_046220
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006701 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ACLA_046220.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004075981 – 435Methionine aminopeptidase 2-2Add BLAST435

Interactioni

Protein-protein interaction databases

STRINGi5057.CADACLAP00004837.

Structurei

3D structure databases

ProteinModelPortaliA1CH02.
SMRiA1CH02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi60 – 71Poly-LysAdd BLAST12

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226278.
KOiK01265.
OMAiTINKHFG.
OrthoDBiEOG092C3NQP.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiView protein in InterPro
IPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF00557. Peptidase_M24. 1 hit.
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiView protein in PROSITE
PS01202. MAP_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A1CH02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQTTEKLQ KLDLNGQSGD AKADAPAAGQ AEAGEAEEDS DDEKDDGNAA
60 70 80 90 100
PEAGAGGAAK KKKRKSKKKK KGGAKVQSSP PRVPVSNLFP NNQYPEGEIV
110 120 130 140 150
EYTNENSYRT TNEEKRYLDR MNNDFLQEYR QAAEVHRQVR QYAQKNIKPG
160 170 180 190 200
QTLTEIAEGI EDAVRALTGH QGLEEGDNLK GGMGFPCGLS INHCAAHYTP
210 220 230 240 250
NAGNKMVLQQ GDVMKVDFGA QINGRIVDSA FTMTFDPVYD PLLEAVKDAT
260 270 280 290 300
NTGIREAGID VRMSDIGAAI QEAMESYEIE LNGTMYPVKC IRNLNGHNID
310 320 330 340 350
QHVIHGGKSV PIVKGGDQTK MEEGETFAIE TFGSTGKGYV REDMETSHYA
360 370 380 390 400
LVPDAPSVPL RLSSAKNLLN VINKNFGTLP FCRRYLDRLG QDKYLLGLNN
410 420 430
LVSSGIVQDY PPLCDIKGSY TAQFEHVQIP SHSHP
Length:435
Mass (Da):47,491
Last modified:January 23, 2007 - v1
Checksum:i2D152BB644700084
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027054 Genomic DNA. Translation: EAW10157.1.
RefSeqiXP_001271583.1. XM_001271582.1.

Genome annotation databases

EnsemblFungiiCADACLAT00004938; CADACLAP00004837; CADACLAG00004938.
GeneIDi4704294.
KEGGiact:ACLA_046220.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMAP22_ASPCL
AccessioniPrimary (citable) accession number: A1CH02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families