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Protein

Probable alpha-L-arabinofuranosidase B

Gene

abfB

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Alpha-L-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysaccharides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan (By similarity).By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Pathwayi: L-arabinan degradation

This protein is involved in the pathway L-arabinan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway L-arabinan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei184 – 185Cis-peptide bondBy similarity2
Binding sitei227SubstrateBy similarity1
Active sitei229NucleophileBy similarity1
Binding sitei230Substrate; via amide nitrogenBy similarity1
Binding sitei304Substrate; via amide nitrogenBy similarity1
Active sitei305Proton donorBy similarity1
Binding sitei424SubstrateBy similarity1
Binding sitei426Substrate; via amide nitrogenBy similarity1
Binding sitei427Substrate; via amide nitrogenBy similarity1
Binding sitei443SubstrateBy similarity1
Binding sitei471SubstrateBy similarity1
Binding sitei476Substrate; via amide nitrogenBy similarity1
Binding sitei496SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00667.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-L-arabinofuranosidase B (EC:3.2.1.55)
Short name:
ABF B
Short name:
Arabinosidase B
Gene namesi
Name:abfB
ORF Names:ACLA_066470
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006701 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ACLA_066470.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000039460327 – 506Probable alpha-L-arabinofuranosidase BAdd BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi29 ↔ 39By similarity
Disulfide bondi89 ↔ 94By similarity
Disulfide bondi184 ↔ 185By similarity
Glycosylationi285N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi409 ↔ 447By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiA1CGD1.

Structurei

3D structure databases

ProteinModelPortaliA1CGD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 343CatalyticBy similarityAdd BLAST317
Regioni344 – 506ABDBy similarityAdd BLAST163

Domaini

Organized into two domains: an N-terminal catalytic domain and a C-terminal arabinose-binding domain (ABD).By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 54 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000187007.
OMAiNIVAAKY.
OrthoDBiEOG092C3Z3T.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR015289. A-L-arabinofuranosidase_B_cat.
IPR007934. AbfB_ABD.
IPR013320. ConA-like_dom.
[Graphical view]
PfamiPF05270. AbfB. 1 hit.
PF09206. ArabFuran-catal. 1 hit.
[Graphical view]
SUPFAMiSSF110221. SSF110221. 1 hit.
SSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1CGD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSQPSRERA FVLALGLVVS SSLAAAAPCD IYSSGGTPCV AAHSTTRALY
60 70 80 90 100
SAYSGPLYQV KRGSDGATTN IAPRSAGGVA NAAAQDTFCA SMTCLITVIY
110 120 130 140 150
DQSGRGNHLT QAPPGGFKGP EANGYDNLAS AIGAPVTLNG QKAYGVFISP
160 170 180 190 200
GTGYRNNAAS GTAIGDAAEG MYAVLDGTHY NGGCCFDYGN AETSSLDTGN
210 220 230 240 250
GHMEAIYFGT NTVWGSGSGS GPWIMADLEN GLFSGSSPAN NAGDPSVSYR
260 270 280 290 300
FLTAAIKGGP NRWAIRGANA ASGSLATYYS GARPNASGYN PMSKEGAIIL
310 320 330 340 350
GIGGDNSNGA QGTFYEGVMT SGYPSDATEN AVQADIVAAN YAVTSLTSGP
360 370 380 390 400
ALTVGSAISL RATTSCCTTR YLAHTGSTIN TQVVSSASAT TLKQQATWTV
410 420 430 440 450
RTGLANSGCF SFESKDTPGS FIRHSNFALV LNGNDGTKQF KEDATFCPQA
460 470 480 490 500
GLNGQGSSIR SWSFPTRYFR HYSNVLYAAS NGGVHTFDAA GSFNDDVSWV

ISSGFA
Length:506
Mass (Da):51,886
Last modified:January 23, 2007 - v1
Checksum:iCDEB3D1906F35DD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027053 Genomic DNA. Translation: EAW11011.1.
RefSeqiXP_001272437.1. XM_001272436.1.

Genome annotation databases

EnsemblFungiiCADACLAT00006371; CADACLAP00006216; CADACLAG00006371.
GeneIDi4704554.
KEGGiact:ACLA_066470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027053 Genomic DNA. Translation: EAW11011.1.
RefSeqiXP_001272437.1. XM_001272436.1.

3D structure databases

ProteinModelPortaliA1CGD1.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA1CGD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADACLAT00006371; CADACLAP00006216; CADACLAG00006371.
GeneIDi4704554.
KEGGiact:ACLA_066470.

Organism-specific databases

EuPathDBiFungiDB:ACLA_066470.

Phylogenomic databases

HOGENOMiHOG000187007.
OMAiNIVAAKY.
OrthoDBiEOG092C3Z3T.

Enzyme and pathway databases

UniPathwayiUPA00667.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR015289. A-L-arabinofuranosidase_B_cat.
IPR007934. AbfB_ABD.
IPR013320. ConA-like_dom.
[Graphical view]
PfamiPF05270. AbfB. 1 hit.
PF09206. ArabFuran-catal. 1 hit.
[Graphical view]
SUPFAMiSSF110221. SSF110221. 1 hit.
SSF49899. SSF49899. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiABFB_ASPCL
AccessioniPrimary (citable) accession number: A1CGD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.