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Protein

Probable alpha-L-arabinofuranosidase B

Gene

abfB

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Alpha-L-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysaccharides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan (By similarity).By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Pathway:iL-arabinan degradation

This protein is involved in the pathway L-arabinan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway L-arabinan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei184 – 1852Cis-peptide bondBy similarity
Binding sitei227 – 2271SubstrateBy similarity
Active sitei229 – 2291NucleophileBy similarity
Binding sitei230 – 2301Substrate; via amide nitrogenBy similarity
Binding sitei304 – 3041Substrate; via amide nitrogenBy similarity
Active sitei305 – 3051Proton donorBy similarity
Binding sitei424 – 4241SubstrateBy similarity
Binding sitei426 – 4261Substrate; via amide nitrogenBy similarity
Binding sitei427 – 4271Substrate; via amide nitrogenBy similarity
Binding sitei443 – 4431SubstrateBy similarity
Binding sitei471 – 4711SubstrateBy similarity
Binding sitei476 – 4761Substrate; via amide nitrogenBy similarity
Binding sitei496 – 4961SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00667.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-L-arabinofuranosidase B (EC:3.2.1.55)
Short name:
ABF B
Short name:
Arabinosidase B
Gene namesi
Name:abfB
ORF Names:ACLA_066470
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006701 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ACLA_066470.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 506480Probable alpha-L-arabinofuranosidase BPRO_0000394603Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 39By similarity
Disulfide bondi89 ↔ 94By similarity
Disulfide bondi184 ↔ 185By similarity
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi409 ↔ 447By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiA1CGD1.

Structurei

3D structure databases

ProteinModelPortaliA1CGD1.
SMRiA1CGD1. Positions 27-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 343317CatalyticBy similarityAdd
BLAST
Regioni344 – 506163ABDBy similarityAdd
BLAST

Domaini

Organized into two domains: an N-terminal catalytic domain and a C-terminal arabinose-binding domain (ABD).By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 54 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG83819.
HOGENOMiHOG000187007.
OMAiNIVAAKY.
OrthoDBiEOG7DFXNQ.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR015289. A-L-arabinofuranosidase_B_cat.
IPR007934. AbfB_ABD.
IPR013320. ConA-like_dom.
[Graphical view]
PfamiPF05270. AbfB. 1 hit.
PF09206. ArabFuran-catal. 1 hit.
[Graphical view]
SUPFAMiSSF110221. SSF110221. 1 hit.
SSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1CGD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSQPSRERA FVLALGLVVS SSLAAAAPCD IYSSGGTPCV AAHSTTRALY
60 70 80 90 100
SAYSGPLYQV KRGSDGATTN IAPRSAGGVA NAAAQDTFCA SMTCLITVIY
110 120 130 140 150
DQSGRGNHLT QAPPGGFKGP EANGYDNLAS AIGAPVTLNG QKAYGVFISP
160 170 180 190 200
GTGYRNNAAS GTAIGDAAEG MYAVLDGTHY NGGCCFDYGN AETSSLDTGN
210 220 230 240 250
GHMEAIYFGT NTVWGSGSGS GPWIMADLEN GLFSGSSPAN NAGDPSVSYR
260 270 280 290 300
FLTAAIKGGP NRWAIRGANA ASGSLATYYS GARPNASGYN PMSKEGAIIL
310 320 330 340 350
GIGGDNSNGA QGTFYEGVMT SGYPSDATEN AVQADIVAAN YAVTSLTSGP
360 370 380 390 400
ALTVGSAISL RATTSCCTTR YLAHTGSTIN TQVVSSASAT TLKQQATWTV
410 420 430 440 450
RTGLANSGCF SFESKDTPGS FIRHSNFALV LNGNDGTKQF KEDATFCPQA
460 470 480 490 500
GLNGQGSSIR SWSFPTRYFR HYSNVLYAAS NGGVHTFDAA GSFNDDVSWV

ISSGFA
Length:506
Mass (Da):51,886
Last modified:January 23, 2007 - v1
Checksum:iCDEB3D1906F35DD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027053 Genomic DNA. Translation: EAW11011.1.
RefSeqiXP_001272437.1. XM_001272436.1.

Genome annotation databases

EnsemblFungiiCADACLAT00006371; CADACLAP00006216; CADACLAG00006371.
GeneIDi4704554.
KEGGiact:ACLA_066470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027053 Genomic DNA. Translation: EAW11011.1.
RefSeqiXP_001272437.1. XM_001272436.1.

3D structure databases

ProteinModelPortaliA1CGD1.
SMRiA1CGD1. Positions 27-506.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA1CGD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADACLAT00006371; CADACLAP00006216; CADACLAG00006371.
GeneIDi4704554.
KEGGiact:ACLA_066470.

Organism-specific databases

EuPathDBiFungiDB:ACLA_066470.

Phylogenomic databases

eggNOGiNOG83819.
HOGENOMiHOG000187007.
OMAiNIVAAKY.
OrthoDBiEOG7DFXNQ.

Enzyme and pathway databases

UniPathwayiUPA00667.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR015289. A-L-arabinofuranosidase_B_cat.
IPR007934. AbfB_ABD.
IPR013320. ConA-like_dom.
[Graphical view]
PfamiPF05270. AbfB. 1 hit.
PF09206. ArabFuran-catal. 1 hit.
[Graphical view]
SUPFAMiSSF110221. SSF110221. 1 hit.
SSF49899. SSF49899. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Entry informationi

Entry nameiABFB_ASPCL
AccessioniPrimary (citable) accession number: A1CGD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.