A1CBW8 (AGALA_ASPCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha-galactosidase A
EC=3.2.1.22

Alternative name(s):
Melibiase A

Gene names

Name:	aglA
ORF Names:	ACLA_016820

Organism

Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]

Taxonomic identifier

344612 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus clavatus

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes a variety of simple alpha-D-galactoside as well as more complex molecules such as oligosaccharides and polysaccharides By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Lectin
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 525	508	Probable alpha-galactosidase A		PRO_0000393213
Regions
	Domain	402 – 525	124	Ricin B-type lectin
Sites
	Active site	148	1	Nucleophile By similarity
	Active site	206	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	43	1	N-linked (GlcNAc...) Potential
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	117	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Disulfide bond	40 ↔ 72		By similarity
	Disulfide bond	120 ↔ 150		By similarity
	Disulfide bond	422 ↔ 434		By similarity
	Disulfide bond	459 ↔ 472		By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1CBW8 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: DBDD564471DEF811	FASTA	525	57,347
	10 20 30 40 50 60 MHPSMTLLAI LPPLVRASIG NPHLLPTPPM GFNNWARFMC NLNESLFLDT AAAMLDTGLH 70 80 90 100 110 120 AAGYTRLNLD DCWMASHRAP NGSLPWDPTK FPHSLPWLSA QLRSLGFSLG IYQDAGNVTC 130 140 150 160 170 180 GGYPGSYGFE ELDAHTFAEW GVDYLKLDGC NVSPAGAVSL ADEYRARYAR WHSVLGAMPH 190 200 210 220 230 240 PLVFSESAPA YFVDPQNATA WYGVMDWVPA YGELARHSTD ILVYEGEGSA WQSIMVNYRY 250 260 270 280 290 300 NTLLARYQRP GYFNDPDFLI ADHPGLSLVE KRSHFALWAS FGAPLIISAD VPGLSKEVIA 310 320 330 340 350 360 VLTNADLIRV DQDALGLQAT LASRSEHLDV LTRSLDGGDR LVTILNRGDG GLAVKVPVGW 370 380 390 400 410 420 MGLQRCAYQA KNLWDGEIQE IEEDIEVQLD SHATEVFRVS LPPDCPMVIP TGIVFNTASG 430 440 450 460 470 480 NCLTDGEALA FEPCRGQDLQ VWQVEESGIL RPLSRTSHCL TAIGDNVVVK PCTGRPGQRW 490 500 510 520 TYHITGNLQN QHTGACLTEG TGIDVCGFEL DSQVFGLPSG VDIEA

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027049 Genomic DNA. Translation: EAW13236.1.
RefSeq	XP_001274662.1. XM_001274661.1.
3D structure databases
ProteinModelPortal	A1CBW8.
ModBase	Search...
Protein-protein interaction databases
STRING	5057.CADACLAP00001524.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADACLAT00001543; CADACLAP00001524; CADACLAG00001543.
GeneID	4706882.
KEGG	act:ACLA_016820.
Phylogenomic databases
eggNOG	NOG68897.
HOGENOM	HOG000161224.
KO	K07407.
OrthoDB	EOG48KVKH.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR017853. Glycoside_hydrolase_SF. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF02065. Melibiase. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27.
SMART	SM00458. RICIN. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF50370. RicinB_like. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. False negative. PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGALA_ASPCL

Accession

Primary (citable) accession number: A1CBW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program