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Protein

Aspergillopepsin-1

Gene

pepA

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions, but also accepts a lysine residue in the P1 position, leading to the activation of trypsinogen and chymotrypsinogen A.By similarity

Catalytic activityi

Hydrolysis of proteins with broad specificity. Generally favors hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101PROSITE-ProRule annotation1
Active sitei283PROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.026.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspergillopepsin-1Curated (EC:3.4.23.18By similarity)
Alternative name(s):
Aspartic protease pepA
Aspergillopepsin I
Aspergillopeptidase A
Gene namesi
Name:pepA
ORF Names:ACLA_016280
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006701 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ACLA_016280.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000040703921 – 69Activation peptideBy similarityAdd BLAST49
ChainiPRO_000040704070 – 394Aspergillopepsin-1Add BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi319 ↔ 354PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA1CBR4.
SMRiA1CBR4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 391Peptidase A1PROSITE-ProRule annotationAdd BLAST307

Sequence similaritiesi

Belongs to the peptidase A1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000167484.
KOiK06004.
OMAiINYGPVT.
OrthoDBiEOG092C49YE.

Family and domain databases

CDDicd06097. Aspergillopepsin_like. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiView protein in InterPro
IPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR034163. Aspergillopepsin-like_cat_dom.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiView protein in Pfam
PF00026. Asp. 1 hit.
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiView protein in PROSITE
PS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1CBR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVFSKVTAA VFGLATIASA APAPPTRKGF TVQQQARPAQ KKQVNLPAMY
60 70 80 90 100
AHALTKFGGS VPESVKVAAS KGSAVTTPEA GDVEYLTPVN VGGTVMNLDF
110 120 130 140 150
DTGSADLWVF SGELPASETS GHSVYKPGRT ASKLPGGSWQ ISYGDGSSAS
160 170 180 190 200
GDVYKDTVVV GGVTAHGQAV EAAAQISSQF LQDKNNDGLL GLAFSSLNTV
210 220 230 240 250
QPQPQTTFFD TVKSSLDRPL FAVTLKHNAP GSFDFGYIDH SKYTGEIAYT
260 270 280 290 300
DVDNSQGFWS FTADGYSIGG GQSSGSSISG IADTGTTLLL LDDNVVSDFY
310 320 330 340 350
QHVEGAQNSD EYGGYVFPCS AKVPSFTTII GGYKAVTPGK LINYGPVTDG
360 370 380 390
SSTCYGGIQS SGGVGQNIFG DIFLKSQFVV FDSEGPRLGF AAQA
Length:394
Mass (Da):40,934
Last modified:January 23, 2007 - v1
Checksum:iC5E9F9851AAEFBA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027049 Genomic DNA. Translation: EAW13182.1.
RefSeqiXP_001274608.1. XM_001274607.1.

Genome annotation databases

EnsemblFungiiCADACLAT00001470; CADACLAP00001451; CADACLAG00001470.
GeneIDi4706595.
KEGGiact:ACLA_016280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027049 Genomic DNA. Translation: EAW13182.1.
RefSeqiXP_001274608.1. XM_001274607.1.

3D structure databases

ProteinModelPortaliA1CBR4.
SMRiA1CBR4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA01.026.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADACLAT00001470; CADACLAP00001451; CADACLAG00001470.
GeneIDi4706595.
KEGGiact:ACLA_016280.

Organism-specific databases

EuPathDBiFungiDB:ACLA_016280.

Phylogenomic databases

HOGENOMiHOG000167484.
KOiK06004.
OMAiINYGPVT.
OrthoDBiEOG092C49YE.

Family and domain databases

CDDicd06097. Aspergillopepsin_like. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiView protein in InterPro
IPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR034163. Aspergillopepsin-like_cat_dom.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiView protein in Pfam
PF00026. Asp. 1 hit.
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiView protein in PROSITE
PS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPEPA_ASPCL
AccessioniPrimary (citable) accession number: A1CBR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.