A1CBE6 (ALG3_ASPCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
EC=2.4.1.258

Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase

Gene names

Name:	alg3
ORF Names:	ACLA_015020

Organism

Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]

Taxonomic identifier

344612 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase		PRO_0000350920
Regions
	Topological domain	1 – 5	5	Lumenal Potential
	Transmembrane	6 – 26	21	Helical; Potential
	Topological domain	27 – 80	54	Cytoplasmic Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Topological domain	102 – 131	30	Lumenal Potential
	Transmembrane	132 – 152	21	Helical; Potential
	Topological domain	153 – 157	5	Cytoplasmic Potential
	Transmembrane	158 – 178	21	Helical; Potential
	Topological domain	179 – 201	23	Lumenal Potential
	Transmembrane	202 – 222	21	Helical; Potential
	Topological domain	223 – 238	16	Cytoplasmic Potential
	Transmembrane	239 – 259	21	Helical; Potential
	Topological domain	260 – 264	5	Lumenal Potential
	Transmembrane	265 – 285	21	Helical; Potential
	Topological domain	286 – 323	38	Cytoplasmic Potential
	Transmembrane	324 – 344	21	Helical; Potential
	Topological domain	345 – 371	27	Lumenal Potential
	Transmembrane	372 – 389	18	Helical; Potential
	Topological domain	390 – 395	6	Cytoplasmic Potential
	Transmembrane	396 – 416	21	Helical; Potential
	Topological domain	417 – 436	20	Lumenal Potential
	Transmembrane	437 – 457	21	Helical; Potential
	Topological domain	458 – 472	15	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	A1CBE6 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: F45080B02C777AAA	FASTA	472	53,247
	10 20 30 40 50 60 MELNDFCIAT IITALIILCE TLSLLLHPGL ELSRKRTNTI GSLGLKPHAW KPALWDLNTI 70 80 90 100 110 120 DNMELKHLLG DLCMNPRHTR WIAPLLILGD AVLCALIIWR VPYTEIDWTT YMQQVSLYLS 130 140 150 160 170 180 GERDYTLIKG STGPLVYPAA HVYIYSILYY LTDEGRDILL AQILFAIPYL ATLAVVMSCY 190 200 210 220 230 240 RQAGAPPFLL LPLVLSKRLH SIFVLRLFND GFAALAMWIA IFLFQKKKWT AGVVVWSTGV 250 260 270 280 290 300 AIKMTLLLLA PAIAVVLVLS LSLQPSIQLG ALAVLIQILL GIPFLSHNTA GYIARSFELT 310 320 330 340 350 360 RQFMFKWTVN WRFVGEELFL SRKFSLALLA LHIALLGLFA TRVWLKPSGS KLPSFVQQLL 370 380 390 400 410 420 QRRYRTVSLS RTFIMRTMLS SLAIGLLCAR SLHYQFFAYL AWATPFLLWQ TGFHPIVVYA 430 440 450 460 470 VCMAQEWAWN IYPSTNGSSL VVILSLAVQV IGILWNANGR QIPENSPKEH VQ

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed: 18404212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027049 Genomic DNA. Translation: EAW13064.1.
RefSeq	XP_001274490.1. XM_001274489.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1CBE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADACLAT00000965; CADACLAP00000946; CADACLAG00000965.
GeneID	4706320.
KEGG	act:ACLA_015020.
Phylogenomic databases
GeneTree	EFGT00050000003598.
OrthoDB	EOG4MPN04.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
KO	K03845.
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG3_ASPCL

Accession

Primary (citable) accession number: A1CBE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program