A1CBC9 (DCL1_ASPCL) Reviewed, UniProtKB/Swiss-Prot
Last modified
March 6, 2013.
Version 44.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Dicer-like protein 1 | ||||
| Gene names |
| ||||
| Organism | Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome] | ||||
| Taxonomic identifier | 344612 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus ›  |
Protein attributes
| Sequence length | 1534 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity. |
| Cofactor | Magnesium or manganese By similarity. |
| Sequence similarities | Belongs to the helicase family. Dicer subfamily. Contains 1 Dicer dsRNA-binding fold domain. Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 2 RNase III domains. |
| Sequence caution | The sequence EAW13047.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Antiviral defense |
| Domain | Repeat |
| Ligand | ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding RNA-binding |
| Molecular function | Antiviral protein Helicase Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological_process | RNA processing Inferred from electronic annotation. Source: InterPro defense response to virusInferred from electronic annotation. Source: UniProtKB-KW regulation of defense response to virusInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activityInferred from electronic annotation. Source: InterPro RNA bindingInferred from electronic annotation. Source: UniProtKB-KW metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1534 | 1534 | Dicer-like protein 1 | PRO_0000306773 | |||||
Regions | |||||||||
| Domain | 130 – 311 | 182 | Helicase ATP-binding | ||||||
| Domain | 456 – 613 | 158 | Helicase C-terminal | ||||||
| Domain | 648 – 738 | 91 | Dicer dsRNA-binding fold | ||||||
| Domain | 1054 – 1199 | 146 | RNase III 1 | ||||||
| Domain | 1250 – 1402 | 153 | RNase III 2 | ||||||
| Domain | 1436 – 1504 | 69 | DRBM | ||||||
| Nucleotide binding | 143 – 150 | 8 | ATP By similarity | ||||||
| Motif | 256 – 259 | 4 | DEAH box | ||||||
Sites | |||||||||
| Metal binding | 1291 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 1388 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 1391 | 1 | Magnesium or manganese By similarity | ||||||
| Site | 1384 | 1 | Important for activity By similarity | ||||||
Sequences
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References
| [1] | "Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.  Nierman W.C.PLoS Genet. 4:E1000046-E1000046(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | DS027049 Genomic DNA. Translation: EAW13047.1. Sequence problems. |
| RefSeq | XP_001274473.1. XM_001274472.1. |
3D structure databases | |
| ProteinModelPortal | A1CBC9. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 4706314. |
| KEGG | act:ACLA_014840. |
Phylogenomic databases | |
| eggNOG | COG1111. |
| HOGENOM | HOG000048448. |
| KO | K11592. |
| OrthoDB | EOG4PRWZS. |
Family and domain databases | |
| Gene3D | 1.10.1520.10. 2 hits. |
| InterPro | IPR005034. Dicer_dsRNA_binding_fold. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000999. RNase_III_dom. [Graphical view] |
| Pfam | PF00270. DEAD. 1 hit. PF03368. dsRNA_bind. 1 hit. PF00271. Helicase_C. 1 hit. PF00636. Ribonuclease_3. 2 hits. [Graphical view] |
| SMART | SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. SM00535. RIBOc. 2 hits. [Graphical view] |
| SUPFAM | SSF69065. RNase_III. 2 hits. |
| PROSITE | PS51327. DICER_DSRBF. 1 hit. PS50137. DS_RBD. False negative. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DCL1_ASPCL | ||||||||
| Accession | Primary (citable) accession number: A1CBC9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |