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A1CBC9

- DCL1_ASPCL

UniProt

A1CBC9 - DCL1_ASPCL

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Protein

Dicer-like protein 1

Gene

dcl1

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons (By similarity).By similarity

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1291 – 12911Magnesium or manganeseBy similarity
Sitei1384 – 13841Important for activityBy similarity
Metal bindingi1388 – 13881Magnesium or manganeseBy similarity
Metal bindingi1391 – 13911Magnesium or manganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi143 – 1508ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. helicase activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. ribonuclease III activity Source: InterPro
  6. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
  2. regulation of defense response to virus Source: UniProtKB-KW
  3. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Helicase, Hydrolase

Keywords - Biological processi

Antiviral defense

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dicer-like protein 1
Including the following 2 domains:
Endoribonuclease dcl1 (EC:3.1.26.-)
ATP-dependent helicase dcl1 (EC:3.6.4.-)
Gene namesi
Name:dcl1
ORF Names:ACLA_014840
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006701: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15341534Dicer-like protein 1PRO_0000306773Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5057.CADACLAP00001180.

Structurei

3D structure databases

ProteinModelPortaliA1CBC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini130 – 311182Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini456 – 613158Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini648 – 73891Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
BLAST
Domaini1054 – 1199146RNase III 1PROSITE-ProRule annotationAdd
BLAST
Domaini1250 – 1402153RNase III 2PROSITE-ProRule annotationAdd
BLAST
Domaini1436 – 150469DRBMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi256 – 2594DEAH box

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1111.
HOGENOMiHOG000048448.
KOiK11592.
OrthoDBiEOG76QFRP.

Family and domain databases

Gene3Di1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1CBC9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSDLISQAE SISNIEILRA LFITDTHTTV GDVAVPSAEP GVEHDQISPG
60 70 80 90 100
ESDEEIEENI SDQNNSSSQK RLQNAQFEAL LTRRAEDTSN ENIDRVPLNL
110 120 130 140 150
SDNELSIAHL VAKQDVGGGL LDPREYQIEL FERAKAQNTI AVLDTGSGKT
160 170 180 190 200
LIAVLLLRHV LQNELNDRAN GKPHRVSFFL VDSVTLAYQQ AAVLRNNIDQ
210 220 230 240 250
NVAHFFGAMG TDLWDRQVWE EHLQQNMVIV CTAEILNQCL LNSHVRMNQI
260 270 280 290 300
NLLIFDEAHH TKKDHPYARI IRDSYFKASP PQRPRIFGMT ASPIDTKGDI
310 320 330 340 350
IAAATRLETL LDSRIATTSK ITLLRQVVSR PIEKVWAYDR LESPFKTNLH
360 370 380 390 400
KLMENRFGNV KALEGVFRFA WYASSELGRW CSDRAWLYAL ADDVLPKLEG
410 420 430 440 450
HVNKLAESTA AATERDMAFK EITLIKEASN IVKAHTFNDP EFPGELSPKV
460 470 480 490 500
RLLQTELSKH FSHAPETKCI IFTQKRYTAK TLHELFTILS IPHLRPGVLI
510 520 530 540 550
GVRSGDIGGM NITFRQQFLA LVKFRKGEIN CLFATSVAEE GLDIPDCNLV
560 570 580 590 600
VRFDLYHTLI QYVQSRGRAR HYHSTYASMV EKDNSDHEAR LREVREAEKT
610 620 630 640 650
MQNFCETLPE DRILHGNDHD LDSLLQHEEG RRTFTVKSTG ARLTYHSAIA
660 670 680 690 700
ILARYASSLQ YEKETTPQAT YVVQSVGNTY VCEVILPEKS PIRGLTGSPA
710 720 730 740 750
IRKSIAKQSA AFDTCLLLRR HKLLDDFFKS IYHKRLPAMR NAKLAITSKK
760 770 780 790 800
TNQYDMLLKP SIWRRHQGIL PSKLYGTILS LLPSEPLSRE HQPILVLTRE
810 820 830 840 850
KLPDFPAFPI YLDEDIETKV VPRSLDTGME LSAEELRALT TFTLRIFRDV
860 870 880 890 900
FHKVYEQESE KLPYWLAPAE PLDANGREPG PRGIIDWKTV TFVQENDEIV
910 920 930 940 950
FSRDLAPESL VNRFMFDKWD GRSRFFTIKV MEGLRAADPP PSSVPRRRHM
960 970 980 990 1000
DNIMSYCLSL SKNSRARFLA GCHWDQPVLQ AELVRLRRNL LDKLTTEEKK
1010 1020 1030 1040 1050
IQTECFICAE PLKISAIPPS IASTCLAFPA IITRLDSYLI SIEACDELDL
1060 1070 1080 1090 1100
VIRSDYALEA VTKDSDNTEE HRGQQIHFQR GMGKNYERLE FLGDCFLKMA
1110 1120 1130 1140 1150
TSIALFTQNP DDDEFDYHVN RMCLICNKNL FNTAKKRQIY RYIRSRSFSR
1160 1170 1180 1190 1200
HVWYPDGLTL LQGKDHSKKM LSQAKHALGE KTIADVCEAL IGACLLSGGP
1210 1220 1230 1240 1250
EHRFDMGVKA VSVFVDSPSH AVSRWKEYIG LYKPPNYQVR KAEGAETNLA
1260 1270 1280 1290 1300
LQVEEKLGYH FRYPRLLCSA VTHPSTPSTW YFRVPCYQRL EFLGDSLLDM
1310 1320 1330 1340 1350
VCVEDLFHRF PDRDPQWLTE HKMAMVSNKF LGALAVKLGF HKHIKAFSNP
1360 1370 1380 1390 1400
LQAQITYYVE EIETAEAESE GAVDYWVVAK DPPKCLPDMV EAYLGAIFVD
1410 1420 1430 1440 1450
SDFSFEVIEA FFQAQIKPYF QDMSIYDTFA NKHPTTFLHN RLANEFGCTN
1460 1470 1480 1490 1500
YCLKAGEMPA IDGMPAGVLA AVIVHNSVVS EATASSSRYA KIRASERALV
1510 1520 1530
VLDGLLPYEF RQRYNCNCQV VGNPASAPDI GTAI
Length:1,534
Mass (Da):174,094
Last modified:October 2, 2007 - v2
Checksum:i200457CEAAB4115B
GO

Sequence cautioni

The sequence EAW13047.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027049 Genomic DNA. Translation: EAW13047.1. Sequence problems.
RefSeqiXP_001274473.1. XM_001274472.1.

Genome annotation databases

GeneIDi4706314.
KEGGiact:ACLA_014840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027049 Genomic DNA. Translation: EAW13047.1 . Sequence problems.
RefSeqi XP_001274473.1. XM_001274472.1.

3D structure databases

ProteinModelPortali A1CBC9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5057.CADACLAP00001180.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4706314.
KEGGi act:ACLA_014840.

Phylogenomic databases

eggNOGi COG1111.
HOGENOMi HOG000048448.
KOi K11592.
OrthoDBi EOG76QFRP.

Family and domain databases

Gene3Di 1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProi IPR005034. Dicer_dimerisation_dom.
IPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view ]
Pfami PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEi PS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Entry informationi

Entry nameiDCL1_ASPCL
AccessioniPrimary (citable) accession number: A1CBC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: November 26, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3