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A1CBC9 (DCL1_ASPCL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dicer-like protein 1

Including the following 2 domains:

  1. Endoribonuclease dcl1
    EC=3.1.26.-
  2. ATP-dependent helicase dcl1
    EC=3.6.4.-
Gene names
Name:dcl1
ORF Names:ACLA_014840
OrganismAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]
Taxonomic identifier344612 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length1534 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.

Cofactor

Magnesium or manganese By similarity.

Sequence similarities

Belongs to the helicase family. Dicer subfamily.

Contains 1 Dicer dsRNA-binding fold domain.

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 2 RNase III domains.

Sequence caution

The sequence EAW13047.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15341534Dicer-like protein 1
PRO_0000306773

Regions

Domain130 – 311182Helicase ATP-binding
Domain456 – 613158Helicase C-terminal
Domain648 – 73891Dicer dsRNA-binding fold
Domain1054 – 1199146RNase III 1
Domain1250 – 1402153RNase III 2
Domain1436 – 150469DRBM
Nucleotide binding143 – 1508ATP By similarity
Motif256 – 2594DEAH box

Sites

Metal binding12911Magnesium or manganese By similarity
Metal binding13881Magnesium or manganese By similarity
Metal binding13911Magnesium or manganese By similarity
Site13841Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A1CBC9 [UniParc].

Last modified October 2, 2007. Version 2.
Checksum: 200457CEAAB4115B

FASTA1,534174,094
        10         20         30         40         50         60 
MSSDLISQAE SISNIEILRA LFITDTHTTV GDVAVPSAEP GVEHDQISPG ESDEEIEENI 

        70         80         90        100        110        120 
SDQNNSSSQK RLQNAQFEAL LTRRAEDTSN ENIDRVPLNL SDNELSIAHL VAKQDVGGGL 

       130        140        150        160        170        180 
LDPREYQIEL FERAKAQNTI AVLDTGSGKT LIAVLLLRHV LQNELNDRAN GKPHRVSFFL 

       190        200        210        220        230        240 
VDSVTLAYQQ AAVLRNNIDQ NVAHFFGAMG TDLWDRQVWE EHLQQNMVIV CTAEILNQCL 

       250        260        270        280        290        300 
LNSHVRMNQI NLLIFDEAHH TKKDHPYARI IRDSYFKASP PQRPRIFGMT ASPIDTKGDI 

       310        320        330        340        350        360 
IAAATRLETL LDSRIATTSK ITLLRQVVSR PIEKVWAYDR LESPFKTNLH KLMENRFGNV 

       370        380        390        400        410        420 
KALEGVFRFA WYASSELGRW CSDRAWLYAL ADDVLPKLEG HVNKLAESTA AATERDMAFK 

       430        440        450        460        470        480 
EITLIKEASN IVKAHTFNDP EFPGELSPKV RLLQTELSKH FSHAPETKCI IFTQKRYTAK 

       490        500        510        520        530        540 
TLHELFTILS IPHLRPGVLI GVRSGDIGGM NITFRQQFLA LVKFRKGEIN CLFATSVAEE 

       550        560        570        580        590        600 
GLDIPDCNLV VRFDLYHTLI QYVQSRGRAR HYHSTYASMV EKDNSDHEAR LREVREAEKT 

       610        620        630        640        650        660 
MQNFCETLPE DRILHGNDHD LDSLLQHEEG RRTFTVKSTG ARLTYHSAIA ILARYASSLQ 

       670        680        690        700        710        720 
YEKETTPQAT YVVQSVGNTY VCEVILPEKS PIRGLTGSPA IRKSIAKQSA AFDTCLLLRR 

       730        740        750        760        770        780 
HKLLDDFFKS IYHKRLPAMR NAKLAITSKK TNQYDMLLKP SIWRRHQGIL PSKLYGTILS 

       790        800        810        820        830        840 
LLPSEPLSRE HQPILVLTRE KLPDFPAFPI YLDEDIETKV VPRSLDTGME LSAEELRALT 

       850        860        870        880        890        900 
TFTLRIFRDV FHKVYEQESE KLPYWLAPAE PLDANGREPG PRGIIDWKTV TFVQENDEIV 

       910        920        930        940        950        960 
FSRDLAPESL VNRFMFDKWD GRSRFFTIKV MEGLRAADPP PSSVPRRRHM DNIMSYCLSL 

       970        980        990       1000       1010       1020 
SKNSRARFLA GCHWDQPVLQ AELVRLRRNL LDKLTTEEKK IQTECFICAE PLKISAIPPS 

      1030       1040       1050       1060       1070       1080 
IASTCLAFPA IITRLDSYLI SIEACDELDL VIRSDYALEA VTKDSDNTEE HRGQQIHFQR 

      1090       1100       1110       1120       1130       1140 
GMGKNYERLE FLGDCFLKMA TSIALFTQNP DDDEFDYHVN RMCLICNKNL FNTAKKRQIY 

      1150       1160       1170       1180       1190       1200 
RYIRSRSFSR HVWYPDGLTL LQGKDHSKKM LSQAKHALGE KTIADVCEAL IGACLLSGGP 

      1210       1220       1230       1240       1250       1260 
EHRFDMGVKA VSVFVDSPSH AVSRWKEYIG LYKPPNYQVR KAEGAETNLA LQVEEKLGYH 

      1270       1280       1290       1300       1310       1320 
FRYPRLLCSA VTHPSTPSTW YFRVPCYQRL EFLGDSLLDM VCVEDLFHRF PDRDPQWLTE 

      1330       1340       1350       1360       1370       1380 
HKMAMVSNKF LGALAVKLGF HKHIKAFSNP LQAQITYYVE EIETAEAESE GAVDYWVVAK 

      1390       1400       1410       1420       1430       1440 
DPPKCLPDMV EAYLGAIFVD SDFSFEVIEA FFQAQIKPYF QDMSIYDTFA NKHPTTFLHN 

      1450       1460       1470       1480       1490       1500 
RLANEFGCTN YCLKAGEMPA IDGMPAGVLA AVIVHNSVVS EATASSSRYA KIRASERALV 

      1510       1520       1530 
VLDGLLPYEF RQRYNCNCQV VGNPASAPDI GTAI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS027049 Genomic DNA. Translation: EAW13047.1. Sequence problems.
RefSeqXP_001274473.1. XM_001274472.1.

3D structure databases

ProteinModelPortalA1CBC9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5057.CADACLAP00001180.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4706314.
KEGGact:ACLA_014840.

Phylogenomic databases

eggNOGCOG1111.
HOGENOMHOG000048448.
KOK11592.
OrthoDBEOG76QFRP.

Family and domain databases

Gene3D1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProIPR005034. Dicer_dimerisation_dom.
IPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDCL1_ASPCL
AccessionPrimary (citable) accession number: A1CBC9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: April 16, 2014
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families