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A1CBC9

- DCL1_ASPCL

UniProt

A1CBC9 - DCL1_ASPCL

Protein

Dicer-like protein 1

Gene

dcl1

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.By similarity

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1291 – 12911Magnesium or manganeseBy similarity
    Sitei1384 – 13841Important for activityBy similarity
    Metal bindingi1388 – 13881Magnesium or manganeseBy similarity
    Metal bindingi1391 – 13911Magnesium or manganeseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi143 – 1508ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: InterPro
    3. helicase activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. ribonuclease III activity Source: InterPro
    6. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. regulation of defense response to virus Source: UniProtKB-KW
    3. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Antiviral protein, Helicase, Hydrolase

    Keywords - Biological processi

    Antiviral defense

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dicer-like protein 1
    Including the following 2 domains:
    Endoribonuclease dcl1 (EC:3.1.26.-)
    ATP-dependent helicase dcl1 (EC:3.6.4.-)
    Gene namesi
    Name:dcl1
    ORF Names:ACLA_014840
    OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
    Taxonomic identifieri344612 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006701: Unassembled WGS sequence

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15341534Dicer-like protein 1PRO_0000306773Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5057.CADACLAP00001180.

    Structurei

    3D structure databases

    ProteinModelPortaliA1CBC9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini130 – 311182Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini456 – 613158Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini648 – 73891Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
    BLAST
    Domaini1054 – 1199146RNase III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1250 – 1402153RNase III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1436 – 150469DRBMAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi256 – 2594DEAH box

    Sequence similaritiesi

    Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
    Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 2 RNase III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1111.
    HOGENOMiHOG000048448.
    KOiK11592.
    OrthoDBiEOG76QFRP.

    Family and domain databases

    Gene3Di1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProiIPR005034. Dicer_dimerisation_dom.
    IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PfamiPF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF04851. ResIII. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEiPS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1CBC9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSDLISQAE SISNIEILRA LFITDTHTTV GDVAVPSAEP GVEHDQISPG     50
    ESDEEIEENI SDQNNSSSQK RLQNAQFEAL LTRRAEDTSN ENIDRVPLNL 100
    SDNELSIAHL VAKQDVGGGL LDPREYQIEL FERAKAQNTI AVLDTGSGKT 150
    LIAVLLLRHV LQNELNDRAN GKPHRVSFFL VDSVTLAYQQ AAVLRNNIDQ 200
    NVAHFFGAMG TDLWDRQVWE EHLQQNMVIV CTAEILNQCL LNSHVRMNQI 250
    NLLIFDEAHH TKKDHPYARI IRDSYFKASP PQRPRIFGMT ASPIDTKGDI 300
    IAAATRLETL LDSRIATTSK ITLLRQVVSR PIEKVWAYDR LESPFKTNLH 350
    KLMENRFGNV KALEGVFRFA WYASSELGRW CSDRAWLYAL ADDVLPKLEG 400
    HVNKLAESTA AATERDMAFK EITLIKEASN IVKAHTFNDP EFPGELSPKV 450
    RLLQTELSKH FSHAPETKCI IFTQKRYTAK TLHELFTILS IPHLRPGVLI 500
    GVRSGDIGGM NITFRQQFLA LVKFRKGEIN CLFATSVAEE GLDIPDCNLV 550
    VRFDLYHTLI QYVQSRGRAR HYHSTYASMV EKDNSDHEAR LREVREAEKT 600
    MQNFCETLPE DRILHGNDHD LDSLLQHEEG RRTFTVKSTG ARLTYHSAIA 650
    ILARYASSLQ YEKETTPQAT YVVQSVGNTY VCEVILPEKS PIRGLTGSPA 700
    IRKSIAKQSA AFDTCLLLRR HKLLDDFFKS IYHKRLPAMR NAKLAITSKK 750
    TNQYDMLLKP SIWRRHQGIL PSKLYGTILS LLPSEPLSRE HQPILVLTRE 800
    KLPDFPAFPI YLDEDIETKV VPRSLDTGME LSAEELRALT TFTLRIFRDV 850
    FHKVYEQESE KLPYWLAPAE PLDANGREPG PRGIIDWKTV TFVQENDEIV 900
    FSRDLAPESL VNRFMFDKWD GRSRFFTIKV MEGLRAADPP PSSVPRRRHM 950
    DNIMSYCLSL SKNSRARFLA GCHWDQPVLQ AELVRLRRNL LDKLTTEEKK 1000
    IQTECFICAE PLKISAIPPS IASTCLAFPA IITRLDSYLI SIEACDELDL 1050
    VIRSDYALEA VTKDSDNTEE HRGQQIHFQR GMGKNYERLE FLGDCFLKMA 1100
    TSIALFTQNP DDDEFDYHVN RMCLICNKNL FNTAKKRQIY RYIRSRSFSR 1150
    HVWYPDGLTL LQGKDHSKKM LSQAKHALGE KTIADVCEAL IGACLLSGGP 1200
    EHRFDMGVKA VSVFVDSPSH AVSRWKEYIG LYKPPNYQVR KAEGAETNLA 1250
    LQVEEKLGYH FRYPRLLCSA VTHPSTPSTW YFRVPCYQRL EFLGDSLLDM 1300
    VCVEDLFHRF PDRDPQWLTE HKMAMVSNKF LGALAVKLGF HKHIKAFSNP 1350
    LQAQITYYVE EIETAEAESE GAVDYWVVAK DPPKCLPDMV EAYLGAIFVD 1400
    SDFSFEVIEA FFQAQIKPYF QDMSIYDTFA NKHPTTFLHN RLANEFGCTN 1450
    YCLKAGEMPA IDGMPAGVLA AVIVHNSVVS EATASSSRYA KIRASERALV 1500
    VLDGLLPYEF RQRYNCNCQV VGNPASAPDI GTAI 1534
    Length:1,534
    Mass (Da):174,094
    Last modified:October 2, 2007 - v2
    Checksum:i200457CEAAB4115B
    GO

    Sequence cautioni

    The sequence EAW13047.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027049 Genomic DNA. Translation: EAW13047.1. Sequence problems.
    RefSeqiXP_001274473.1. XM_001274472.1.

    Genome annotation databases

    GeneIDi4706314.
    KEGGiact:ACLA_014840.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027049 Genomic DNA. Translation: EAW13047.1 . Sequence problems.
    RefSeqi XP_001274473.1. XM_001274472.1.

    3D structure databases

    ProteinModelPortali A1CBC9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5057.CADACLAP00001180.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4706314.
    KEGGi act:ACLA_014840.

    Phylogenomic databases

    eggNOGi COG1111.
    HOGENOMi HOG000048448.
    KOi K11592.
    OrthoDBi EOG76QFRP.

    Family and domain databases

    Gene3Di 1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProi IPR005034. Dicer_dimerisation_dom.
    IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    Pfami PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF04851. ResIII. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEi PS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

    Entry informationi

    Entry nameiDCL1_ASPCL
    AccessioniPrimary (citable) accession number: A1CBC9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3