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A1C9M6 (DCL2_ASPCL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dicer-like protein 2

Including the following 2 domains:

  1. Endoribonuclease dcl2
    EC=3.1.26.-
  2. ATP-dependent helicase dcl2
    EC=3.6.4.-
Gene names
Name:dcl2
ORF Names:ACLA_055980
OrganismAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]
Taxonomic identifier344612 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length1389 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.

Cofactor

Magnesium or manganese By similarity.

Sequence similarities

Belongs to the helicase family. Dicer subfamily.

Contains 1 Dicer dsRNA-binding fold domain.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 2 RNase III domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13891389Dicer-like protein 2
PRO_0000306785

Regions

Domain23 – 203181Helicase ATP-binding
Domain368 – 537170Helicase C-terminal
Domain564 – 65895Dicer dsRNA-binding fold
Domain920 – 1060141RNase III 1
Domain1099 – 1282184RNase III 2
Nucleotide binding36 – 438ATP By similarity
Motif144 – 1474DEAH box

Sites

Metal binding11381Magnesium or manganese By similarity
Metal binding12681Magnesium or manganese By similarity
Metal binding12711Magnesium or manganese By similarity
Site12641Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A1C9M6 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: EA8AAF2FA35F7D55

FASTA1,389156,806
        10         20         30         40         50         60 
MSSAVPTNSD AAAYQARNYQ LEMLEASMKE NIIVAMDTGS GKTHIAVLRI KAELDSCPPD 

        70         80         90        100        110        120 
KIIWFLAPTV ALCTQQHKVI ASNLPAVQTR TLTGLDKVEL WTEQAIWDAI LKDVRVVVST 

       130        140        150        160        170        180 
YAVLADALSH GFMRMSRLAL IIFDEAHHCM RKHAANKIMQ DFYHPTVSKF GPSAVPRIMG 

       190        200        210        220        230        240 
LTASPVVRSN REELFTIETN LDAVCKTPRA HRQELLKFSH RPELKQLLYE PPDPMGLQVS 

       250        260        270        280        290        300 
SQTLRALIEA WETLDIEDDP YVKRLRKSSL DGGALEKALM TGKTFCREQL KRFVDRSRHI 

       310        320        330        340        350        360 
FEELGEWAAD YYIYTSIQHL KTRVQTSYMT GDWDEAERAY LVSFLSKLPA ADIQITLSDA 

       370        380        390        400        410        420 
ACLRISPKLE ALIGFLDAMD DPEFSGLIFV KQRATVSVMT DLLAVHPRTR ERFRSAAYVG 

       430        440        450        460        470        480 
WSNSSGSKDF LGNLLSMHGQ LSTLDDFRSG HKNLIIATDV LEEGIDISAC SVVVCYDKPP 

       490        500        510        520        530        540 
NLKSFVQRRG RARQKQSTYA IMFPAEDSAF DLAKWQTLEQ AMIEAYQDDE RHLQSAIELE 

       550        560        570        580        590        600 
RVNEEVVERF TVESTSAVLT ADTAMAHLHH FCAVLPSQPY VDMRPVFSVE TDIANLRRGI 

       610        620        630        640        650        660 
VILPNCVHPK VRRSEGQRWW RTERAAMKEA AFQAYKSLYE FGLVNDNLLP LTNKPELKVN 

       670        680        690        700        710        720 
KLGSIPSIME ASEQYDPWVD WAYSWSSPDI HQSRVVVRLN GAKDRELCMR LAGPTVLPPL 

       730        740        750        760        770        780 
APMTLFWDSE TTFTVAFEAP ERVPHVPLSS VEDMRTSTVV YLQATSSRLL STKQDFTALF 

       790        800        810        820        830        840 
GPDLPHTDLK AWLQMYEGNE PAVEVYSRRR DPMLMGVVRD CSRYNEPLLF RRWVESGDSP 

       850        860        870        880        890        900 
TTSVVEMECD PFPRRRNLLH RQTLATKKLE NDKEDTPEPV NKMRTVAADQ CTIDRLPFSN 

       910        920        930        940        950        960 
AILGLFISVV VQQLETELIA TKLYETILRD VGFTSTQHII TAISAPSAQA LTNYQRYEFL 

       970        980        990       1000       1010       1020 
GDSILKFSVS CQLFFKHPNW HEGYLSEGRD EIVQNPRLTK AALDTGLDAF VITKMFTPRK 

      1030       1040       1050       1060       1070       1080 
WSAPLISERL ERVAGKRQVS SKVLADVVEA LIGAAYMDGG HAAAQACIRR FLPEINLHSL 

      1090       1100       1110       1120       1130       1140 
DTRTLSRSVA PEGARQTIHD RLKRHISYTF ENESLLVEAL THPSCDYDAS TQSYQRLEFL 

      1150       1160       1170       1180       1190       1200 
GDAVLDMIIV SAIFNHPVQM PQGHMTKIKH ALVNANLLAF LCMEFAIPEE RASVEQISTL 

      1210       1220       1230       1240       1250       1260 
QFEVISNEEL VELWRFMRYR GLDLNNARDA SLARHRALRD EILHSLQHDP HYPWQALSRL 

      1270       1280       1290       1300       1310       1320 
SADKFFSDIM ESILGAIFVD SGGDLAPCEA FVERIGLMSY LRRILDENIE VTHPRNVAQQ 

      1330       1340       1350       1360       1370       1380 
LAKSNIQFLA QRMPDEEGGA SYQCAVRIEE VEAFVVRGCL TAEEAEVTAA IDAIDLLMER 


VNGSASVAS 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS027048 Genomic DNA. Translation: EAW13550.1.
RefSeqXP_001274976.1. XM_001274975.1.

3D structure databases

ProteinModelPortalA1C9M6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5057.CADACLAP00005115.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADACLAT00005226; CADACLAP00005115; CADACLAG00005226.
GeneID4707091.
KEGGact:ACLA_055980.

Phylogenomic databases

eggNOGCOG1111.
HOGENOMHOG000048683.
OMAHPRNIAQ.
OrthoDBEOG76QFRP.

Family and domain databases

Gene3D1.10.1520.10. 2 hits.
3.40.50.300. 2 hits.
InterProIPR005034. Dicer_dimerisation_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000999. RNase_III_dom.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 3 hits.
PROSITEPS51327. DICER_DSRBF. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDCL2_ASPCL
AccessionPrimary (citable) accession number: A1C9M6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families