Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A1C9M6

- DCL2_ASPCL

UniProt

A1C9M6 - DCL2_ASPCL

Protein

Dicer-like protein 2

Gene

dcl2

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons By similarity.By similarity

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1138 – 11381Magnesium or manganeseBy similarity
    Sitei1264 – 12641Important for activityBy similarity
    Metal bindingi1268 – 12681Magnesium or manganeseBy similarity
    Metal bindingi1271 – 12711Magnesium or manganeseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi36 – 438ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. ribonuclease III activity Source: InterPro
    5. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. regulation of defense response to virus Source: UniProtKB-KW
    3. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Antiviral protein, Helicase, Hydrolase

    Keywords - Biological processi

    Antiviral defense

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dicer-like protein 2
    Including the following 2 domains:
    Endoribonuclease dcl2 (EC:3.1.26.-)
    ATP-dependent helicase dcl2 (EC:3.6.4.-)
    Gene namesi
    Name:dcl2
    ORF Names:ACLA_055980
    OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
    Taxonomic identifieri344612 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006701: Unassembled WGS sequence

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13891389Dicer-like protein 2PRO_0000306785Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5057.CADACLAP00005115.

    Structurei

    3D structure databases

    ProteinModelPortaliA1C9M6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 203181Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini368 – 537170Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini564 – 65895Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
    BLAST
    Domaini920 – 1060141RNase III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1099 – 1282184RNase III 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi144 – 1474DEAH box

    Sequence similaritiesi

    Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
    Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 2 RNase III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1111.
    HOGENOMiHOG000048683.
    OMAiCKTPRAH.
    OrthoDBiEOG76QFRP.

    Family and domain databases

    Gene3Di1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProiIPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEiPS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1C9M6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSAVPTNSD AAAYQARNYQ LEMLEASMKE NIIVAMDTGS GKTHIAVLRI     50
    KAELDSCPPD KIIWFLAPTV ALCTQQHKVI ASNLPAVQTR TLTGLDKVEL 100
    WTEQAIWDAI LKDVRVVVST YAVLADALSH GFMRMSRLAL IIFDEAHHCM 150
    RKHAANKIMQ DFYHPTVSKF GPSAVPRIMG LTASPVVRSN REELFTIETN 200
    LDAVCKTPRA HRQELLKFSH RPELKQLLYE PPDPMGLQVS SQTLRALIEA 250
    WETLDIEDDP YVKRLRKSSL DGGALEKALM TGKTFCREQL KRFVDRSRHI 300
    FEELGEWAAD YYIYTSIQHL KTRVQTSYMT GDWDEAERAY LVSFLSKLPA 350
    ADIQITLSDA ACLRISPKLE ALIGFLDAMD DPEFSGLIFV KQRATVSVMT 400
    DLLAVHPRTR ERFRSAAYVG WSNSSGSKDF LGNLLSMHGQ LSTLDDFRSG 450
    HKNLIIATDV LEEGIDISAC SVVVCYDKPP NLKSFVQRRG RARQKQSTYA 500
    IMFPAEDSAF DLAKWQTLEQ AMIEAYQDDE RHLQSAIELE RVNEEVVERF 550
    TVESTSAVLT ADTAMAHLHH FCAVLPSQPY VDMRPVFSVE TDIANLRRGI 600
    VILPNCVHPK VRRSEGQRWW RTERAAMKEA AFQAYKSLYE FGLVNDNLLP 650
    LTNKPELKVN KLGSIPSIME ASEQYDPWVD WAYSWSSPDI HQSRVVVRLN 700
    GAKDRELCMR LAGPTVLPPL APMTLFWDSE TTFTVAFEAP ERVPHVPLSS 750
    VEDMRTSTVV YLQATSSRLL STKQDFTALF GPDLPHTDLK AWLQMYEGNE 800
    PAVEVYSRRR DPMLMGVVRD CSRYNEPLLF RRWVESGDSP TTSVVEMECD 850
    PFPRRRNLLH RQTLATKKLE NDKEDTPEPV NKMRTVAADQ CTIDRLPFSN 900
    AILGLFISVV VQQLETELIA TKLYETILRD VGFTSTQHII TAISAPSAQA 950
    LTNYQRYEFL GDSILKFSVS CQLFFKHPNW HEGYLSEGRD EIVQNPRLTK 1000
    AALDTGLDAF VITKMFTPRK WSAPLISERL ERVAGKRQVS SKVLADVVEA 1050
    LIGAAYMDGG HAAAQACIRR FLPEINLHSL DTRTLSRSVA PEGARQTIHD 1100
    RLKRHISYTF ENESLLVEAL THPSCDYDAS TQSYQRLEFL GDAVLDMIIV 1150
    SAIFNHPVQM PQGHMTKIKH ALVNANLLAF LCMEFAIPEE RASVEQISTL 1200
    QFEVISNEEL VELWRFMRYR GLDLNNARDA SLARHRALRD EILHSLQHDP 1250
    HYPWQALSRL SADKFFSDIM ESILGAIFVD SGGDLAPCEA FVERIGLMSY 1300
    LRRILDENIE VTHPRNVAQQ LAKSNIQFLA QRMPDEEGGA SYQCAVRIEE 1350
    VEAFVVRGCL TAEEAEVTAA IDAIDLLMER VNGSASVAS 1389
    Length:1,389
    Mass (Da):156,806
    Last modified:January 23, 2007 - v1
    Checksum:iEA8AAF2FA35F7D55
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027048 Genomic DNA. Translation: EAW13550.1.
    RefSeqiXP_001274976.1. XM_001274975.1.

    Genome annotation databases

    EnsemblFungiiCADACLAT00005226; CADACLAP00005115; CADACLAG00005226.
    GeneIDi4707091.
    KEGGiact:ACLA_055980.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS027048 Genomic DNA. Translation: EAW13550.1 .
    RefSeqi XP_001274976.1. XM_001274975.1.

    3D structure databases

    ProteinModelPortali A1C9M6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5057.CADACLAP00005115.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADACLAT00005226 ; CADACLAP00005115 ; CADACLAG00005226 .
    GeneIDi 4707091.
    KEGGi act:ACLA_055980.

    Phylogenomic databases

    eggNOGi COG1111.
    HOGENOMi HOG000048683.
    OMAi CKTPRAH.
    OrthoDBi EOG76QFRP.

    Family and domain databases

    Gene3Di 1.10.1520.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProi IPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 3 hits.
    PROSITEi PS51327. DICER_DSRBF. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

    Entry informationi

    Entry nameiDCL2_ASPCL
    AccessioniPrimary (citable) accession number: A1C9M6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3