ID   NTE1_ASPCL              Reviewed;        1528 AA.
AC   A1C9L6;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   16-JUN-2009, entry version 17.
DE   RecName: Full=Lysophospholipase nte1;
DE            EC=3.1.1.5;
DE   AltName: Full=Neuropathy target esterase homolog;
DE   AltName: Full=Intracellular phospholipase B;
GN   Name=nte1; ORFNames=ACLA_055880;
OS   Aspergillus clavatus.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae;
OC   mitosporic Trichocomaceae; Aspergillus.
OX   NCBI_TaxID=5057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P.,
RA   Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A.,
RA   Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A.,
RA   Galens K., Fraser-Liggett C.M., Haas B.J., Inman J.M., Kent R.,
RA   Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M.,
RA   Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R.,
RA   Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R.,
RA   Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Intracellular phospholipase B that catalyzes the double
CC       deacylation of phosphatidylcholine (PC) to glycerophosphocholine
CC       (GroPCho). Plays an important role in membrane lipid homeostasis.
CC       Responsible for the rapid PC turnover in response to inositol,
CC       elevated temperatures, or when choline is present in the growth
CC       medium (By similarity).
CC   -!- CATALYTIC ACTIVITY: 2-lysophosphatidylcholine + H(2)O =
CC       glycerophosphocholine + a carboxylate.
CC   -!- ENZYME REGULATION: Inhibited by organophosphorus esters (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein (By similarity).
CC   -!- SIMILARITY: Belongs to the NTE family.
CC   -!- SIMILARITY: Contains 2 cyclic nucleotide-binding domains.
CC   -!- SIMILARITY: Contains 1 patatin domain.
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DR   EMBL; DS027048; EAW13540.1; ALT_INIT; Genomic_DNA.
DR   BRENDA; 3.1.1.5; 18580.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004622; F:lysophospholipase activity; IEA:EC.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0046470; P:phosphatidylcholine metabolic process; IEA:InterPro.
DR   InterPro; IPR000595; cNMP_bd.
DR   InterPro; IPR018488; cNMP_bd_CS.
DR   InterPro; IPR001423; Lysophospholipase_patatin_CS.
DR   InterPro; IPR002641; Patatin.
DR   Pfam; PF00027; cNMP_binding; 2.
DR   Pfam; PF01734; Patatin; 1.
DR   SMART; SM00100; cNMP; 1.
DR   PROSITE; PS00888; CNMP_BINDING_1; FALSE_NEG.
DR   PROSITE; PS00889; CNMP_BINDING_2; FALSE_NEG.
DR   PROSITE; PS50042; CNMP_BINDING_3; 2.
DR   PROSITE; PS01237; UPF0028; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Endoplasmic reticulum; Hydrolase;
KW   Lipid degradation; Lipid metabolism; Membrane; Repeat; Transmembrane.
FT   CHAIN         1   1528       Lysophospholipase nte1.
FT                                /FTId=PRO_0000295309.
FT   TOPO_DOM      1     72       Cytoplasmic (By similarity).
FT   TRANSMEM     73     93       Potential.
FT   TOPO_DOM     94    115       Lumenal (By similarity).
FT   TRANSMEM    116    136       Potential.
FT   TOPO_DOM    137   1528       Cytoplasmic (By similarity).
FT   DOMAIN     1225   1389       Patatin.
FT   NP_BIND     686    805       cNMP 1.
FT   NP_BIND     846    966       cNMP 2.
FT   MOTIF      1256   1260       GXSXG.
FT   COMPBIAS     21     33       Poly-Ser.
FT   COMPBIAS     59     64       Poly-Pro.
FT   COMPBIAS    298    307       Poly-Ser.
FT   ACT_SITE   1258   1258       By similarity.
SQ   SEQUENCE   1528 AA;  168937 MW;  D5982DE223A05DD9 CRC64;
     MADGNLLGSS TSLTALLPTP SSASLSASLS SSSLPVSPFL APAPTTAITA SIASLSAQPP
     PPLPATPATM AGWIGWVFSF FFQFIPSVLY SVITFTTITL PTWLFTLFSM SLTFTMNFTT
     LLLILLAVVS TLGWFVRYRF LNMYSRLPPE PQRKEPQIDL FPDVQGGDSK PGLANYLDEF
     LSAIKVFGYL ERPVFHELTR TMQTRKLIAG ETLMLEEEKG FCLVVDGLVQ IFVKSTRDGK
     SGSDDELHHL GAESSDEEHH IDGKQGYQLL TEVKNGASMS SLFSILSLFT EDIQVWDSQS
     STSSSSSIAM RAARVPDSTP NSPRGGMDSP TPIFRDVPDP VSLVNENGDL PLVPPLHLEE
     SPIPPTNHAH DRRQHDHRKH HGRKHRKSVH PDIVARAMVD TTIAIIPASA FRRLTRVYPR
     ATAHIVQVIL TRLQRVTFAT AHSYLGLSNE VLGIEKQMTK FTTYDLPNNM RGTALDRLKD
     KFIKERDRLG TEEVTKGIAL HNPSAGRRRR SSSFMRKDAV LHAKMMSPKR AATVITSDNS
     YDHDSAGVSP GDLLSTIQQS RFGPRYEQPT PRLRSPLAEK ENSHFRLPAM QARNAFHRKE
     SLDEDALFRE CILDCIMKAI GLTSSTGEVL RKSSHSGEAS PKLLSYDSRR QKAVFSNNAF
     GFIDPYEGSG DGETESMMSM SVTSAGGTSP VTSLREELRN DIEIVYFPQG SVLVEQGERH
     PGLYYVIDGF LDVGMPVVDK GEDLVGVSKP ATAREPFPTL KRTTTASSIK PSATAANDPR
     RRKQSRKSLY LIKPGGIQGY VGAVASYRSY TDVVAKTDVY VGFLPRASLE RIAERYPIAL
     LTLAKRLTSL LPRLLLHIDF ALEWVQVNAG QVIYHQGDES DAIYLVLNGR LRSVLESADN
     KLTVIGEYGQ GESVGELEVM TESTRPATLH AIRDTELAKF PRSLFNSLAQ EHPGITIQVS
     KLIAQRMRDL VERPVTEKGA ERSSAGGVQT ATSTLNLRTV GILPVTAGVP VVEFGNRLLH
     ALHQIGVVNG VTSLNQSAIL NHLGRHAFSK MGKLKLAQYL ADLEEKYGMV LYIADTNVNS
     PWTQTCITQA DCILLVGLAE SSPSIGEYER FLLGMKTTAR KELVLLHSER YCPPGLTRRW
     LKNRVWINGG HHHIQMAFRL TAEPSHPETK RFGTVLKQRV QVLQAEIQKY TSRRIRQTPL
     YSAQTPFKGD FHRLARRLCG RAVGLVLGGG GARGIAHVGV IKALEEAGIP VDIIGGTSIG
     SFIGALYARD ADVVPMYGRA KKFAGRMGSM WRFALDLTYP TISYTTGHEF NRGIFKTFGD
     SQIEDFWLEF YCNTTNISKS RQEYHSSGYV WRYVRASMSL AGLIPPICDE GSMLLDGGYI
     DNLTVDHMKG LGADVIFAVD VGSIDDNTPQ GYGDSLSGFW VAFNRWNPFS SCPNPPTLSE
     IQARLAYVSS IDNLERAKIT PGCLYMRPPI DAYGTLEFGK FDEIYQVGYK FGKQFLEKLK
     NEGSLPLPEE TEEEKKLLRT MAPRRASI
//