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A1C8U0 (MANF_ASPCL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannan endo-1,4-beta-mannosidase F

EC=3.2.1.78
Alternative name(s):
Endo-beta-1,4-mannanase F
Gene names
Name:manF
ORF Names:ACLA_044470
OrganismAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]
Taxonomic identifier344612 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans By similarity.

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Subcellular location

Secreted By similarity.

Domain

Has a modular structure: a carbohydrate-binding module (CBM) at the N-terminus, a linker rich in serines, and a C-terminal endo-1,4-mannanase catalytic module. The genes for catalytic modules and CBMs seem to have evolved separately and have been linked by gene fusion.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulose binding

Inferred from electronic annotation. Source: InterPro

mannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 436418Mannan endo-1,4-beta-mannosidase F
PRO_0000393712

Regions

Domain19 – 5436CBM1
Region79 – 11335Ser-rich linker
Region114 – 436323Catalytic

Sites

Active site2611Proton donor By similarity
Active site3701Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
A1C8U0 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 5183B090F2AF6246

FASTA43647,428
        10         20         30         40         50         60 
MRSLSSVALL SAIGAASAQA GPWGQCAGIS HTGPTTCESG WSCVYLNDWY SQCQPGAATS 

        70         80         90        100        110        120 
SSTTVSSTKQ PSSTVAAPSS TTSAHTLPTG SGSFAKTDGL KFNIDGKTKY FAGTNAYWLP 

       130        140        150        160        170        180 
FLTNNADVDA VFDHLQQTGL KILRTWGFND VNTIPGSGTV YFQLHDKATG TSTINTGANG 

       190        200        210        220        230        240 
LQRLDYVISA AEKHGIKLII PFVNNWDDYG GMNAYINAYG GSKTEWYTNE KIQSVYQAYI 

       250        260        270        280        290        300 
KAIVSRYRDS PAIFAWELGN EPRCKGCSTD VIYNWVAKTS AYIKSLDPNH MVTTGEEGMG 

       310        320        330        340        350        360 
LTVDSDGSYP YSKDEGSDFA RNLAAPDIDF GVYHLYVADW GVSDNAWGNR WIKSHAKVCE 

       370        380        390        400        410        420 
AAGKPCLFEE YGIKDDHCGD SLKWQKTSLT TTANSADLFW QYGQQLSTGA SPNDHYTIYY 

       430 
GTDDWKCAVI DHISQI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS027046 Genomic DNA. Translation: EAW13727.1.
RefSeqXP_001275153.1. XM_001275152.1.

3D structure databases

ProteinModelPortalA1C8U0.
SMRA1C8U0. Positions 20-54, 92-436.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADACLAT00004242; CADACLAP00004144; CADACLAG00004242.
GeneID4707278.
KEGGact:ACLA_044470.

Phylogenomic databases

eggNOGCOG3934.
HOGENOMHOG000169951.
OMAEKNLAIP.
OrthoDBEOG7M3J90.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000254. Cellulose-bd_dom_fun.
IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00734. CBM_1. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
ProDomPD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEPS51164. CBM1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMANF_ASPCL
AccessionPrimary (citable) accession number: A1C8U0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries