A1C8R3 (KATG_ASPCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
ORF Names:	ACLA_044200

Organism

Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) [Complete proteome]

Taxonomic identifier

344612 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. 2 H2O2 = O2 + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 760	760	Catalase-peroxidase		PRO_0000354098
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	283	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 242		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-268) By similarity
	Cross-link	242 ↔ 268		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1C8R3 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: F4ED7671BC8562C4	FASTA	760	83,843
	10 20 30 40 50 60 MAESKCPFKS QGSRSNVAGG GTRNTDWWPE QLKLNILRQH TTVTNPLGAD FDYAAAFNTL 70 80 90 100 110 120 DYDALKKDLT ALMTDSQEWW PADFGHYGGL FIRMAWHSAG TYRVFDGRGG AGQGQQRFAP 130 140 150 160 170 180 LNSWPDNVSL DKARRLLWPI KQKYGDKISW ADLMILTGNV ALESMGFKTF GFAGGRADTW 190 200 210 220 230 240 EADESAYWGR ETTWLGNDAR YEKGFSGSDK QGTVIADEAS HKTTHSRELE NPLAAAHMGL 250 260 270 280 290 300 IYVNPEGPDG NPDPVAAAHD IRVTFGRMAM NDEETVALIA GGHTFGKTHG AAPADNVGKE 310 320 330 340 350 360 PEAAGLEAQG LGWQNSHGSG KGPDTITSGL EVTWTKTPTK WSNQFLEYLF KFDWELTKSP 370 380 390 400 410 420 AGAHQWVAKN ADDIIPDAYD AFKKHKPTML TTDLSLRFDP AYEKISRRFL ENPDQFADAF 430 440 450 460 470 480 ARAWFKLTHR DMGPRARYLG PEVPGEVLLW QDPIPAVNHA LIDTVDTAAL KRDVLATGVN 490 500 510 520 530 540 PSKFISTAWA AASTFRGSDK RGGANGARIR FAPQRSWEVN NQPWLQESLS ALEGVQSRFN 550 560 570 580 590 600 ASRPDRKQVS LADLIVLAGC AAVEQAAHDA GFPVRVPFTP GRMDASQDET DVESFSHMEP 610 620 630 640 650 660 IADGFRNYAK PYVHGRAEHY LVDKAQLLNL SAPEMTVLVG GLRVLNTNYD GSAHGVFTSR 670 680 690 700 710 720 PGVLSNDFFV NLLDMNTAWQ AGHNGEIFEG ADRKSGAKKW TATRADLVFG SHAELRAVAE 730 740 750 760 VYASADGQRK FVNDFVAAWN KVMNLDRFDL QGKQFIYPRL

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed: 18404212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027046 Genomic DNA. Translation: EAW13700.1.
RefSeq	XP_001275126.1. XM_001275125.1.
3D structure databases
ProteinModelPortal	A1C8R3.
SMR	A1C8R3. Positions 21-750.
ModBase	Search...
Protein-protein interaction databases
STRING	A1C8R3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADACLAT00004125; CADACLAP00004027; CADACLAG00004125.
GeneID	4707337.
KEGG	act:ACLA_044200.
Phylogenomic databases
GeneTree	EFGT00050000003307.
OrthoDB	EOG41CB4B.
Family and domain databases
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ASPCL

Accession

Primary (citable) accession number: A1C8R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program