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Protein

Cytosolic Fe-S cluster assembly factor nbp35

Gene

nbp35

Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The nbp35-cfd1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of nbp35 and two labile, bridging clusters between subunits of the nbp35-cfd1 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi44 – 441Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi47 – 471Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi257 – 2571Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi260 – 2601Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 918ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor nbp35UniRule annotation
Alternative name(s):
Nucleotide-binding protein 35UniRule annotation
Gene namesi
Name:nbp35
ORF Names:ACLA_074930
OrganismiAspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1)
Taxonomic identifieri344612 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006701 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ACLA_074930.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Cytosolic Fe-S cluster assembly factor nbp35PRO_0000278887Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of 2 nbp35 and 2 cfd1 chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA1C7T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000079916.
OMAiFEMIPPM.
OrthoDBiEOG70CRJC.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35.
MF_03038. NUBP1.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1C7T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSLVEPVP AFDAPSKVAP NLVAPEPEHC PGPESEQAGK GDACAGCPNQ
60 70 80 90 100
AICASSTPKG PDPDIPIITE RLSQIRHKIL VLSGKGGVGK STFSSLLAHA
110 120 130 140 150
FASNPESTVG LCDTDICGPS IPKMMGVEAE TIHVSNAGWS PVWVTDNLGA
160 170 180 190 200
MSIQFMLPNR DDAVIWRGPK KNGLIKQFLK DVDWGELDYL IVDTPPGTSD
210 220 230 240 250
EHLSVNTLLK ESGVDGAVIV TTPQEVSLLD VRKEIDFCRK AGIRILGLVE
260 270 280 290 300
NMRGFVCPGC SNTSEIFRAT TGGGKRLAKK MGIPFLGSVP LDPRVGMACD
310 320 330 340
YGESFVDSYP DSPASMAIKQ VVRSVSQMIG EDPDKVLPPD VMAE
Length:344
Mass (Da):36,507
Last modified:January 23, 2007 - v1
Checksum:i96102AC10B13CA85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027045 Genomic DNA. Translation: EAW14455.1.
RefSeqiXP_001275881.1. XM_001275880.1.

Genome annotation databases

EnsemblFungiiCADACLAT00006711; CADACLAP00006551; CADACLAG00006711.
GeneIDi4707697.
KEGGiact:ACLA_074930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS027045 Genomic DNA. Translation: EAW14455.1.
RefSeqiXP_001275881.1. XM_001275880.1.

3D structure databases

ProteinModelPortaliA1C7T4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADACLAT00006711; CADACLAP00006551; CADACLAG00006711.
GeneIDi4707697.
KEGGiact:ACLA_074930.

Organism-specific databases

EuPathDBiFungiDB:ACLA_074930.

Phylogenomic databases

HOGENOMiHOG000079916.
OMAiFEMIPPM.
OrthoDBiEOG70CRJC.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35.
MF_03038. NUBP1.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Entry informationi

Entry nameiNBP35_ASPCL
AccessioniPrimary (citable) accession number: A1C7T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: January 23, 2007
Last modified: November 11, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.