Reviewed, UniProtKB/Swiss-Prot A1C7E9 (NCB5R_ASPCL)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
    EC=1.6.2.2
Alternative name(s):
    Microsomal cytochrome b reductase

Gene names

Name:	cbr1
ORF Names:	ACLA_073550

Organism

Aspergillus clavatus [Complete proteome]

Taxonomic identifier

5057 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	NADH-cytochrome b5 reductase 1		PRO_0000330144
Regions
	Transmembrane	29 – 49	21	Potential
	Domain	60 – 165	106	FAD-binding FR-type
	Nucleotide binding	145 – 160	16	FAD By similarity
	Nucleotide binding	171 – 208	38	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1C7E9-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 214157BBE69E47B3	FASTA	309	33,772
	10 20 30 40 50 60 MSALSSENVN GVYIPSALLV FGTFLVKKEF VPYAVALTAV LAGFKLFTGD SKARKVLNPT 70 80 90 100 110 120 EFQEFVLKEK TDISHNVSIY RFALPRPTDI LGLPIGQHIS LAATIEGQPK EVVRSYTPIS 130 140 150 160 170 180 SDNEAGYFDL LVKAYPQGNI SKHLTTLKVG DVMKVRGPKG AMVYTPNMCR HIGMIAGGTG 190 200 210 220 230 240 ITPMLQVIKA IIRNRPRNGG TDITKVDLIF ANVNPEDILL KEELDKLAAE DEDFNIYYVL 250 260 270 280 290 300 NNPPQGWTGG VGFVTPEMIK ERLPAPASDV KVLLCGPPPM ISAMKKATES LGFTKARPVS KLEDQVFCF

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed: 18404212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS027045 Genomic DNA. Translation: EAW14320.1.
RefSeq	XP_001275746.1.
3D structure databases
SMR	A1C7E9. Positions 52-296.
ModBase	Search...
Genome annotation databases
GeneID	4707891.
Phylogenomic databases
OrthoDB	EOG9KM0KS.
PhylomeDB	A1C7E9.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_ASPCL

Accession

Primary (citable) accession number: A1C7E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)