Reviewed, UniProtKB/Swiss-Prot A1C6D6 (ATG15_ASPCL)

Names and origin

Protein names

Recommended name:
    Putative lipase atg15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	atg15
ORF Names:	ACLA_069880

Organism

Aspergillus clavatus [Complete proteome]

Taxonomic identifier

5057 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	Putative lipase atg15		PRO_0000317958
Regions
	Topological domain	1 – 20	20	Cytoplasmic By similarity
	Transmembrane	21 – 40	20	Signal-anchor for type II membrane protein
	Topological domain	41 – 630	590	Lumenal By similarity
	Compositional bias	470 – 587	118	Thr-rich
Sites
	Active site	320	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Glycosylation	304	1	N-linked (GlcNAc...) Potential
	Glycosylation	466	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A1C6D6-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: D43F76BCDDE74A4D	FASTA	630	68,898
	10 20 30 40 50 60 MKSSQRRIKR HAMRDMSIST LLLSVVLLPS VVSANDHVYF NPPSPGSPFL GPQIPLTGPP 70 80 90 100 110 120 SLTNEHEFTL RHIYERGTND QPDLHRRLDI KPHTRLWAVS DDGLEKELVT FDTPLVASSS 130 140 150 160 170 180 PLTIQRLADR RPSVIEGYLT AARYNGEAVA LSSSDWVMDT LAGPDVTKKE TVLTFAKMTA 190 200 210 220 230 240 NDYIEEPGTE DWNYIHGRFN YSSSFGWQSD GLRGHIYADT KNNTIVISLK GTSPALFDGA 250 260 270 280 290 300 GTTTNDKIND NLFFSCCCGQ GGSYLWRQVC DCQQSAFTAN LTCIAEAMND ENKYYRAAID 310 320 330 340 350 360 LYTNVTDMYP DANVWMTGHS LGGAMSSLLG LTFGLPVVTF EAVPEALPAA RLGLPSPPGH 370 380 390 400 410 420 DPRLPQTRKY TGTYHFGHTA DPVYMGTCNG VGSICTWGGY AMESACHTGQ MCVYDTVEDK 430 440 450 460 470 480 GWRVALSTHR IKAVISDVLE VYDNVPPCAA EEECYDCELW KFFRSNGSET TTTSRTSTTT 490 500 510 520 530 540 TPTTTRTLTC ETPGWWGCLD ESTTTTATTA TSTTTTTSTC KTPGWFGCKD STTTVDATAA 550 560 570 580 590 600 PTVTTTIATP TTFPISSTTC KDPGWFGCRD PSSTTASVTA PPFSTSTSSD HVRADHSIGD 610 620 630 GAAHPLTRMY LERLRQVEFA WGSDIEHYEI

References

[1]

"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus." Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J., Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H., Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M. , Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J., Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C., White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H., Wortman J.R., Jiang B., Denning D.W., Nierman W.C. PLoS Genet. 4:E1000046-E1000046(2008) [PubMed: 18404212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS027045 Genomic DNA. Translation: EAW13957.1.
RefSeq	XP_001275383.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4707621.
Enzyme and pathway databases
BRENDA	3.1.1.3. 18580.
Family and domain databases
InterPro	IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_ASPCL

Accession

Primary (citable) accession number: A1C6D6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)