Reviewed, UniProtKB/Swiss-Prot A1BJY3 (MURE_CHLPD)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	Cpha266_2726

Organism

Chlorobium phaeobacteroides (strain DSM 266) [Complete proteome] [HAMAP]

Taxonomic identifier

290317 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012345
Regions
	Nucleotide binding	114 – 120	7	ATP Potential
	Region	156 – 157	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	419 – 422	4	Meso-diaminopimelate binding By similarity
	Motif	419 – 422	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	32	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	183	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	191	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	395	1	Meso-diaminopimelate By similarity
	Binding site	469	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	473	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	223	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1BJY3-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 3C1A45609E6947C1	FASTA	512	55,401
	10 20 30 40 50 60 MSLADLIRQI PVLEAAGGST TSLPVSMISS DSRDVLPGAL FIAVRGYSAD GHRFIKSAVE 70 80 90 100 110 120 RGAAAVLCEE IPPVLPDASL PYLRVEDARQ ALALAARQFY GFASDALHVI GVTGTNGKTT 130 140 150 160 170 180 TARLITAMLN ACGIRTGYIG TNLCTFGEQQ IPLDRTTPEA HILHALFRRM LDSGCSVAVM 190 200 210 220 230 240 EVSSHALVLQ RVYGITFHAA VFTNLSMEHL DFHKTMQEYA GAKRQLFEQL SPQGFAVINS 250 260 270 280 290 300 DDPRAEEMVS KINPEKRFCC TLDQTGRVAG DASRRFSAEL IDQSIASATI RLCFPDAQQT 310 320 330 340 350 360 ISTVLPGQYN VMNLLQAAAV GCGMGLGSPV VAAALDALPG VAGRMERIMD RRRKEFVFVD 370 380 390 400 410 420 YAHTPDALFK ALSTLNALKS PASRLIVVFG CGGNRDRLKR PEMGRIAVEN ADLVILTSDN 430 440 450 460 470 480 PRDEDPEMII DEIEKGIRVK NHKRITDRAQ AIRTAVALLQ PGDVLLVAGK GHEQYQETAG 490 500 510 EKSFFSDQET LRNALAEENA GEPEKGAVCK GC

References

[1]

"Complete sequence of Chlorobium phaeobacteroides DSM 266." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000492 Genomic DNA. Translation: ABL66710.1.
RefSeq	YP_913134.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1BJY3.
Genome annotation databases
GeneID	4570700.
GenomeReviews	Gene locus Cpha266_2726 in contig CP000492_GR.
KEGG	cph:Cpha266_2726.
NMPDR	fig|290317.7.peg.2847.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FPVIVDY.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_CHLPD

Accession

Primary (citable) accession number: A1BJY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)