A1BJY0 (MURD_CHLPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	Cpha266_2723

Organism

Chlorobium phaeobacteroides (strain DSM 266) [Complete proteome] [HAMAP]

Taxonomic identifier

290317 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000301421
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A1BJY0 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 5F9511410FFC9105	FASTA	464	50,600
	10 20 30 40 50 60 MDVAGKKVAV LGARRSGMAV AELLSLKGAS VFVSELGTIG AHESARLQAL RIPFEEGGHS 70 80 90 100 110 120 DNVLCTDFCV ISPGIPGRAP VVRAMLEKGI PLFSEIEVAY WFCKARIIGI TGTDGKTTTA 130 140 150 160 170 180 TLVHRIFETD GMTHRYRAFS VGNIGQPFSS RVLTMRPEDV AVIELSSYQL EGCCTFRPDV 190 200 210 220 230 240 SVITNITPDH LDRYEGELHN YAQTKYRIYA HQGKEDTLVY NDDDPLLHDH FASNRETLPC 250 260 270 280 290 300 RIVPFGIGCK PEHAGFAAAV RFCDHRIVFD AGAKSENIIE AEDFLKRSFR GRHNIANALA 310 320 330 340 350 360 AVAAARALGI GNEAIRSALQ GFSGVEHRQE FVRTLDGSDW INDSKATNIN ALSQALETVP 370 380 390 400 410 420 GRMVLIAGGR DKGSDYREIA GIVRKKVAAL VAIGEAREKL CAAYGGMVDV RSANSLEEAV 430 440 450 460 SLARALVEPG QTVLFSPGCS SFDMFEDFED RGRQFKKLTT DLRS

References

[1]

"Complete sequence of Chlorobium phaeobacteroides DSM 266." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 266.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000492 Genomic DNA. Translation: ABL66707.1.
RefSeq	YP_913131.1. NC_008639.1.
3D structure databases
ProteinModelPortal	A1BJY0.
ModBase	Search...
Protein-protein interaction databases
STRING	A1BJY0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4570697.
GenomeReviews	Gene locus Cpha266_2723 in contig CP000492_GR.
KEGG	cph:Cpha266_2723.
NMPDR	fig|290317.7.peg.2844.
PATRIC	21394229. VBIChlPha122104_3260.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG750024.
OMA	ACASWDM.
PhylomeDB	A1BJY0.
ProtClustDB	PRK02472.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CHLPD

Accession

Primary (citable) accession number: A1BJY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program