A1BHM3 (ISPE_CHLPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Cpha266_1884

Organism

Chlorobium phaeobacteroides (strain DSM 266) [Complete proteome] [HAMAP]

Taxonomic identifier

290317 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007831
Regions
	Nucleotide binding	93 – 103	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	135	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1BHM3 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 154ECBC7B4E0418D	FASTA	294	31,951
	10 20 30 40 50 60 MDHLSVKSFA KINLGLLITG KRKDGYHTLE TIFAPINWYD TIGFSDSDVI SMSCSNIDLP 70 80 90 100 110 120 VDDNNLCIRA ARALQQSASC SKGAAMNLQK VVPFGAGLGG GSSDAATVLR VLNELWKINV 130 140 150 160 170 180 SSAELHELAV KLGADVPYFL EMKGLAFARG IGDELEDLGL TLPFHVVTVF PEEHISTVWA 190 200 210 220 230 240 YKNFYQKFDR PVPDLRLLLQ RLCLDGDRSV LGAFENDFEP AVFDHYPKVR VVKESLLDAG 250 260 270 280 290 SFYASLSGSG SAVFGLFDTL ENAAGAVCAM QQKGYRVTLT PPGFSMEAQA GSRL

References

[1]

"Complete sequence of Chlorobium phaeobacteroides DSM 266." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 266.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000492 Genomic DNA. Translation: ABL65900.1.
RefSeq	YP_912324.1. NC_008639.1.
3D structure databases
ProteinModelPortal	A1BHM3.
ModBase	Search...
Protein-protein interaction databases
STRING	A1BHM3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4571226.
GenomeReviews	Gene locus Cpha266_1884 in contig CP000492_GR.
KEGG	cph:Cpha266_1884.
NMPDR	fig|290317.7.peg.1986.
PATRIC	21392142. VBIChlPha122104_2234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	VSTAEVY.
PhylomeDB	A1BHM3.
ProtClustDB	PRK14616.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CHLPD

Accession

Primary (citable) accession number: A1BHM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program