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A1BFY3

- LIPA_CHLPD

UniProt

A1BFY3 - LIPA_CHLPD

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Protein
Lipoyl synthase
Gene
lipA, Cpha266_1277
Organism
Chlorobium phaeobacteroides (strain DSM 266)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi43 – 431Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi49 – 491Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi64 – 641Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi68 – 681Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCPHA290317:GHX4-1305-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Cpha266_1277
OrganismiChlorobium phaeobacteroides (strain DSM 266)
Taxonomic identifieri290317 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
ProteomesiUP000008701: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Lipoyl synthaseUniRule annotation
PRO_1000012208Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290317.Cpha266_1277.

Structurei

3D structure databases

ProteinModelPortaliA1BFY3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiRKVDCDI.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A1BFY3-1 [UniParc]FASTAAdd to Basket

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MSNVLARKPD WLKLRLSAHG EFAATRQLLE QRNLNTVCRS AMCPNLQECW    50
SRGTATFLLL GNICTRTCRF CAVGTASIPP MPDSLEPENI AEAVEIMNLN 100
HVVLTSVNRD DLADGGARHW QKTMQAVRQR NPKVTLECLI PDFQAQTEAL 150
DIVLAEAPEV LNHNIETVPS IYHIVRPEAN YSSSLNIIRR AKEHFNLTTK 200
SGLMVGMGET FDEVVQSLHD LVQHGCDMVT IGQYLQPSAS HIPVNRYVTP 250
EEFDRYKTVA ESLGLRNVRS GPFVRSSYLA ETLSPDH 287
Length:287
Mass (Da):32,109
Last modified:January 23, 2007 - v1
Checksum:iB26560A3FCADADC5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000492 Genomic DNA. Translation: ABL65310.1.
RefSeqiYP_911734.1. NC_008639.1.

Genome annotation databases

EnsemblBacteriaiABL65310; ABL65310; Cpha266_1277.
GeneIDi4570487.
KEGGicph:Cpha266_1277.
PATRICi21390615. VBIChlPha122104_1477.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000492 Genomic DNA. Translation: ABL65310.1 .
RefSeqi YP_911734.1. NC_008639.1.

3D structure databases

ProteinModelPortali A1BFY3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290317.Cpha266_1277.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABL65310 ; ABL65310 ; Cpha266_1277 .
GeneIDi 4570487.
KEGGi cph:Cpha266_1277.
PATRICi 21390615. VBIChlPha122104_1477.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi RKVDCDI.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci CPHA290317:GHX4-1305-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Chlorobium phaeobacteroides DSM 266."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 266.

Entry informationi

Entry nameiLIPA_CHLPD
AccessioniPrimary (citable) accession number: A1BFY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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