A1BEZ2 (SAHH_CHLPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosylhomocysteinase
EC=3.3.1.1

Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	Cpha266_0921

Organism

Chlorobium phaeobacteroides (strain DSM 266) [Complete proteome] [HAMAP]

Taxonomic identifier

290317 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine By similarity. HAMAP MF_00563
Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000024723
Regions
	Nucleotide binding	197 – 199	3	NAD By similarity
	Nucleotide binding	260 – 265	6	NAD By similarity
	Nucleotide binding	339 – 341	3	NAD By similarity
Sites
	Binding site	60	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity
	Binding site	231	1	NAD By similarity
	Binding site	283	1	NAD By similarity
	Binding site	318	1	NAD By similarity
	Binding site	387	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1BEZ2 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: DF203E7DBF07DD60	FASTA	471	52,434
	10 20 30 40 50 60 MTIEAEVLAY KVADMSLAEW GRKEIDIAEK EMPGLMATRK KYAGQKPLKG ARIAGSLHMT 70 80 90 100 110 120 IQTAVLIETL VELGADVRWA SCNIFSTQDH AAAAIAKAGI PVFAWKGETL DEYWWCTRQI 130 140 150 160 170 180 LEFEGGKGPH LIVDDGGDAT LMIHLGYKIE IDPSLLDKTP GNIEEKALFQ QLREVYEEDS 190 200 210 220 230 240 QRWHNVAAEM KGVSEETTTG VHRLYQMMEK EELLFPAINV NDSVTKSKFD NLYGCRESLA 250 260 270 280 290 300 DGIKRATDVM VAGKVVVVLG YGDVGKGSAR SMRAYGARVI VTEIDPICAL QAAMEGYEVN 310 320 330 340 350 360 TMDEAVTEGN IFVTTTGNKD VITLEHMKKM KGEAIICNIG HFDNEIQVEP LNNYAGATKL 370 380 390 400 410 420 NIKPQVDKYT FEDGHAIYLL AEGRLVNLGC ATGHPSFVMS NSFTNQTLAQ LELWTRDYAI 430 440 450 460 470 DVYRLPKQLD EEVARLHLEQ LGVKLTTLTN EQAEYIGVPL EGPYKPEHYR Y

References

[1]

"Complete sequence of Chlorobium phaeobacteroides DSM 266." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 266.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000492 Genomic DNA. Translation: ABL64969.1.
RefSeq	YP_911393.1. NC_008639.1.
3D structure databases
ProteinModelPortal	A1BEZ2.
SMR	A1BEZ2. Positions 10-471.
ModBase	Search...
Protein-protein interaction databases
STRING	A1BEZ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4570555.
GenomeReviews	Gene locus Cpha266_0921 in contig CP000492_GR.
KEGG	cph:Cpha266_0921.
NMPDR	fig|290317.7.peg.978.
PATRIC	21389771. VBIChlPha122104_1065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0499.
HOGENOM	HBG352029.
OMA	SAQVWVT.
PhylomeDB	A1BEZ2.
ProtClustDB	PRK05476.
Family and domain databases
HAMAP	MF_00563. AdoHcyase. [Tree]
InterPro	IPR000043. Adenosylhomocysteinase. IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
KO	K01251.
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMART	SM00996. AdoHcyase. 1 hit. SM00997. AdoHcyase_NAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00936. AhcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_CHLPD

Accession

Primary (citable) accession number: A1BEZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program