A1BES8 (ILVD_CHLPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Cpha266_0854

Organism

Chlorobium phaeobacteroides (strain DSM 266) [Complete proteome] [HAMAP]

Taxonomic identifier

290317 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000974
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A1BES8 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: C0EB3707B04D0370	FASTA	565	59,598
	10 20 30 40 50 60 MRSDTIKKGF DKAPHRSLLK ATGVITSSDD YQKPFIGICN SFNELIPGHA HLQELGRIAK 70 80 90 100 110 120 NEVRKAGGIP FEFNTIGVCD GIAMGHIGMR YSLASRELIA DSVETVVEAH RLDGIVCIPN 130 140 150 160 170 180 CDKITPGMMM AALRVNIPVI FVSGGPMKAG HTPDGKTVDL ISVFEAVGRH STAEITDGEL 190 200 210 220 230 240 QTIEENACPG CGSCSGMFTA NSMNCLSEAL GLALPGNGTI LASDPRRNEL VKEASRKIID 250 260 270 280 290 300 LVRSNTRPRD ILSRKALLNA FALDFAMGGS TNTILHTLAI ANEAELDFDF SELNALSAKT 310 320 330 340 350 360 PYICKVSPAT MAVHIEDVDR AGGVSAILLE LSKIDGLLDL SAPTVSGKTL GENIAGAEIK 370 380 390 400 410 420 DEKVIRTIDN PYSATGGLAV LYGNLAPQGA VVKTGAVSPS MMRHTGPAKV FDCQDDAIKG 430 440 450 460 470 480 IMEDIIKPGD VVVIRYEGPK GGPGMPEMLS PTSAIMGRGL GDSVALITDG RFSGGSRGAC 490 500 510 520 530 540 IGHVSPEAAE NGPIAALKNG DMITIDIPAR TISVDLSTEA INERIALLPV FEPKIKKGYL 550 560 ARYAQLVTSA CTGAILKTSP YCEPK

References

[1]

"Complete sequence of Chlorobium phaeobacteroides DSM 266." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 266.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000492 Genomic DNA. Translation: ABL64905.1.
RefSeq	YP_911329.1. NC_008639.1.
3D structure databases
ProteinModelPortal	A1BES8.
ModBase	Search...
Protein-protein interaction databases
STRING	A1BES8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4570448.
GenomeReviews	Gene locus Cpha266_0854 in contig CP000492_GR.
KEGG	cph:Cpha266_0854.
NMPDR	fig|290317.7.peg.909.
PATRIC	21389623. VBIChlPha122104_0992.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	A1BES8.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CHLPD

Accession

Primary (citable) accession number: A1BES8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program